Cell culture reagents were obtained from Invitrogen (Darmstadt, Germany). The anti-pERK-1/2 (E-4) and the anti-Rap-1a (sc-1482) antiserum was from Santa Cruz (Heidelberg, Germany). The pCREB-Ser-133 antibody was purchased from cell signaling (Leiden, the Netherlands). The anti-tubulin (clone 6-11B-1) and the peroxidase-conjugated anti-mouse or anti-rabbit antibody, both raised in goat, from Bio-Rad (München, Germany). The firefly luciferase substrate was from Promega (Mannheim, Germany). rp-Br-cAMPs and γ-MSH were from SigmaAldrich (Deisenhofen, Germany). ESI-09, ESI-05 and HJC-0197 were from Biolog (Bremen, Germany), A-812511 from abcam (Cambridge, UK) and PD-184,352, HS-024 or NPY from Tocris (Bristol, UK). α-MSH was from Biotrend (Cologne, Germany) and KT-5720 from Enzo life science (Lörrach, Germany).
Pcreb ser 133 antibody
Manufactured by Cell Signaling Technology
Sourced in Netherlands
The PCREB-Ser-133 antibody is a primary antibody that specifically recognizes the phosphorylated form of the CREB (cAMP Response Element Binding) protein at serine 133. This antibody is used to detect the activated, phosphorylated state of CREB, which is an important transcription factor involved in various cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
Firefly luciferase substrate, by Promega (1 mentions)
Kt5720, by Enzo Life Sciences (1 mentions)
Pd184352, by Bio-Techne (1 mentions)
Esi 09, by Biolog (1 mentions)
γ msh, by Merck Group (1 mentions)
Esi 05, by Biolog (1 mentions)
Hjc0197, by Biolog (1 mentions)
Hs 024, by Bio-Techne (1 mentions)
Cell culture reagents, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 488 goat anti mouse igg, by Abcam (1 mentions)
Fv3000, by Olympus (1 mentions)
2 protocols using pcreb ser 133 antibody
1
Evaluation of Cellular Signaling Modulators
2
Immunolabeling of Phosphorylated CREB and NF-κB
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cells were seed in confocal dishes and treated with/without LPS and 4a before fixed with 4% paraformaldehyde. The membrane was permeabilized using 0.5% Triton X-100. And then the membrane was blocked in 10% goat serum. Antibodies against p-CREBSer133 antibody (Cell Signaling Technology, 9198S), p65 NF-κB antibody (Cell Signaling Technology, 8242T), p-p65 NF-κBSer536 antibody (Cell Signaling Technology, 3033S) were applied on the cells. After washing the dishes three times, the cells were then incubated with the Alexa Fluor 488 goat anti-mouse IgG (green, Abcam), Alexa Fluor 655 anti-rabbit IgG (red, Abcam) for 30 min. Immunolabeling was examined by laser scanning confocal microscope (Olympus, FV3000).
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