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2 protocols using lpo assay kit

1

Oxidative Stress Biomarker Quantification

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LPO assay kit (A106-1-2), glutathione assay kit (A006-2-1), Fe2+/Fe3+ iron assay kit (A039-2-1), and the fluorescent probe CM-H2DCFDA (E004-1-1) were purchased from Jiancheng Bioengineering Institute (Nanjing, China). H2O2 was measured with a Hydrogen Peroxide kit (Beyotime, Shanghai, China). The level of glutathione peroxidase 4 (Anti-GPX4, Abcam, UK) was quantified by immunofluorescence staining.
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2

Oxidative Stress Analysis Protocol

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Pyrrole, 4-carbomethoxybenzaldehyde, propionic acid, MnCl2·4H2O, tetrahydrofuran (THF), ZrOCl2·8H2O, benzoic acid, DMF and 4'6-diamidino-2-phenylindole (DAPI) were purchased from Sinopharm Chemical Reagent Co. Ltd (Beijing, China). RPMI 1640 and fetal bovine serum (FBS) were purchased from Gibco RBL/Life Technologies (Grand Island, NY, USA). SOSG was obtained from Thermo Fisher Scientific Inc. (Waltham, MA, USA). 2'7'-DCFH-DA was purchased from Sigma-Aldrich Company (St Louis, MO, USA). GSH assay kit, GPX assay kit and LPO assay kit were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). All other regents were of analytical grade and used without any further purification.
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