ErbB3 aptamer sequence is 5'-CAGCGAAAGUUGCGUAUGGGUCACAUC GCAGGCACAUGUCAUCUGGGCG-3', FAM labled no-target siRNA (FAMsRNA) sequence is: sense strand 5'-UUCUCCGAACGUGUCACGUdTdT, antisense strand 3'-dTdT AAGAGGCUUGCACAGUGCA, siEGFP sequence is: sense strand 5'-GC UGACCCUGAAGUUCAUCdTdT, antisense strand 3'-dTdT CGACUGGGACUUCA AGUAG, sisurvivin sequence is: sense strand 5'-GGACCACCGCAUCUCUACA dTdT, antisense strand 3'-dTdTCCUGGUGGCGUAGAGAUGU-5'. siCDK1 sequence is: sense strand 5'-ACUUCGUCAUCCAAAUAUAdTdT, antisense strand 3'dTdTUGAAGCAGUAGGUUUAUAU. Dissolved protamine (Sigma-Aldrich, USA) into 0.025µ g/µl with sterile PBS, ErbB 3 aptamer into 0.01 nmol/µl, siRNA (FAMsRNA, siEGFP and sisurvivin, synthesized by Ribobio company, China) into 0.01 nmol/µl, then mixed aptamer and siRNA with the same volume, put the mixture into protamine solution (the volume of protamine was the same as aptamer or siRNA) and mixed them with slow shake, the APR nanoparticles were prepared 30 minutes before using.
Cell culture MCF-7 breast cancer cells and AGS gastric cancer cells (ATCC, USA) were cultured in DMEM medium (Invitrogen, USA), supplemented with 10% bovine serum (Hyclone, USA), the temperature was adjusted at 37℃ with 5% CO2 concentration.
Cell culture MCF-7 breast cancer cells and AGS gastric cancer cells (ATCC, USA) were cultured in DMEM medium (Invitrogen, USA), supplemented with 10% bovine serum (Hyclone, USA), the temperature was adjusted at 37℃ with 5% CO2 concentration.