Glucose 6phosphate dehydrogenase g6pdh

R-(+)-and S-(-)-CAR were obtained from Toronto Research Chemicals (Toronto, Canada). Propranolol hydrochloride, used as an internal standard for highperformance liquid chromatography (HPLC) analysis, and Hanks' Balanced Salt Solution were obtained from Sigma Aldrich (St. Louis, MO, USA). NADP + , glucose-6-phosphate (G6P), and glucose-6phosphate dehydrogenase (G6PDH) were purchased from Oriental Yeast (Tokyo, Japan). All other chemicals and solvents were of HPLC grade or the highest purity available.

Recombinant rat CYP enzymes (CYP1A2, CYP2C11, CYP2C12, CYP2D1, CYP2D2, CYP3A1, and CYP3A2) expressed in baculovirus-infected insect cells, insect cell control microsomes, and pooled 168-donor Sprague Dawley male rat liver microsomes (RLM) were purchased from BD Gentest (Wobum, MA, USA) and BD Biosciences Discovery Labware (Billerica, MA, USA), respectively. R-(+)and S-(-)-CAR were obtained from Toronto Research Chemicals (Toronto, Canada). Propranolol hydrochloride, as an internal standard for high-performance liquid chromatography (HPLC) analysis, furafylline, α-naphthoflavone, and quinine were obtained from Sigma Aldrich (St. Louis, MO, USA). Ketoconazole was purchased from BD Gentest. NADP + , glucose-6-phosphate (G6P), and glucose-6-phosphate dehydrogenase (G6PDH) were purchased from Oriental Yeast (Tokyo, Japan). All other chemicals used were of the highest purity available.