Soy phosphatidylcholine

Manufactured by Avanti Polar Lipids

Sourced in United States

Soy phosphatidylcholine is a naturally occurring lipid extracted from soybeans. It is a key component of cell membranes and plays a vital role in various biological processes. This lab equipment product provides a reliable source of this essential compound for research and scientific applications.

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Lab products found in correlation

Cholesterol, by Merck Group (3 mentions) Hydrogenated soy phosphatidylcholine, by Avanti Polar Lipids (2 mentions) 96 well plate, by Merck Group (1 mentions) Distearoylphosphatidylcholine, by Avanti Polar Lipids (1 mentions) Dimyristoylphosphatidylcholine, by Avanti Polar Lipids (1 mentions) Paclitaxel, by Cayman Chemical (1 mentions) Polysorbate 80, by Merck Group (1 mentions) Perillyl alcohol, by Merck Group (1 mentions) Tributyrin, by Merck Group (1 mentions) Phosphate buffered saline tablet, by Merck Group (1 mentions) Mouse monoclonal antibody against β actin, by Santa Cruz Biotechnology (1 mentions) 1 1 dioctadecyl 3 3 3 3 tetramethylindocarbocyanine perchlorate, by Merck Group (1 mentions) Sybr select master mix, by Thermo Fisher Scientific (1 mentions) Mouse anti il 1β, by Santa Cruz Biotechnology (1 mentions) Mouse anti tnf α, by Santa Cruz Biotechnology (1 mentions) Foetal calf serum, by Euroclone (1 mentions) M mlv reverse transcriptase, by Thermo Fisher Scientific (1 mentions) Mouse anti β actin, by Santa Cruz Biotechnology (1 mentions) Trizol, by Thermo Fisher Scientific (1 mentions) Hplc grade solvent, by Merck Group (1 mentions)

Close spelling variants of this product

Phosphatidylcholine (82 citations) Hydrogenated soy phosphatidylcholine (7 citations) Soy phosphatidylcholine (PC) (7 citations) L-α-phosphatidylcholine (Soy) (2 citations) Hydrogenated soy L-α-phosphatidylcholine (HSPC), (2 citations) Hydrogenated soy phosphatidylcholine (HSPC) (2 citations) Soy phosphatidylcholine (SPC) (2 citations)

7 protocols using soy phosphatidylcholine

Liposomal Bupivacaine Preparation Procedure

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The liposomes were formed using a dehydration-rehydration protocol, as
previously described by Maguire et al. [24 (link)]. Briefly, hydrogenated soy phosphatidylcholine (Avanti Polar
Lipids, Alabaster, AL) and cholesterol were combined in a 7:3 M ratio in a round
bottom flask to create a lipid and dried via rotovap under vacuum. The lipid
film was re-suspended with distilled water and incubated while spinning for 2 h
at 55 °C. The resulting solution was snap frozen and lyophilized
overnight. The lipid was then suspended in 70 mg/mL bupivacaine-HCl and extruded
through polycarbonate membranes 21 times to produce small 200-μm
unilamellar liposomes. The solution was eluted through a Sephadex G-50 size
exclusion column with 0.9% saline to purify the liposomes.

Davis M.S., Marrero-Berrios I., Perez I., Maguire T., Radhakrishnan P., Manchikalapati D., SchianodiCola J., Kamath H., Schloss R.S, & Yarmush J. (2018). Alginate-liposomal construct for bupivacaine delivery and MSC function regulation. Drug delivery and translational research, 8(1), 226-238.

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Tacrolimus Formulation and Pharmacokinetics

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Tacrolimus was purchased from Tecoland Corporation (Irvine, CA, USA). Soy phosphatidylcholine (SPC), distearoyl-phosphatidylcholine (DSPC), hydrogenated Soy phosphatidylcholine (HSPC), dimyristoylphosphatidylcholine (DMPC), and dimyristoylphosphatidylglycerol sodium (DMPG) were purchased from Avanti Polar Lipids (Alabaster, AL, USA); cholesterol and Krebs-Hensleit buffer (pH 7.4) were purchased from Sigma Aldrich (St. Louis, MO, USA); and 96-well plates were purchased from Millipore (Millipore, Billerica, USA). Cannulated Sprague-Dawley (SD) rats were purchased from Harlan Laboratories (Indianapolis, IN, USA). Blank rat plasma was purchased from Valley Biomedical (Winchester, VA, USA). Hard gelatin capsules (size 1) were purchased from Capsugel Inc (Morristown, NJ, USA), and all reagents used were of analytical grade.

Nekkanti V., Rueda J., Wang Z, & Betageri G.V. (2016). Design, Characterization, and In Vivo Pharmacokinetics of Tacrolimus Proliposomes. AAPS PharmSciTech, 17(5).

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Valsartan Lipid Nanoparticle Formulation

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Valsartan was purchased from Tecoland Corporation, (Irvine, CA, USA). Soy phosphatidylcholine (SPC), distearoyl phosphatidylcholine (DSPC), hydrogenated Soy phosphatidylcholine (HSPC), dimyristoylphosphatidylcholine (DMPC), and dimyristoyl phosphatidylglycerol sodium (DMPG) were purchased from Avanti Polar Lipids (Alabaster, AL, USA); cholesterol was purchased from Sigma-Aldrich, (St. Louis, MO, USA). Capmul MCM was purchased from Abitech Corporation (Janesville, WI, USA). Labrafil M 2125 was purchased from Alfa Chemicals (Binfield, Berkshire, UK). Tween 80 was purchased from EMD (Billerica, Massachussetts, USA). Avicel PH102 was purchased from FMC BioPolymers (Philadelphia, PA USA). Cell culture media were purchased from ATC Collection (Manassas, VA, USA); Transwell® plates (6-well) were purchased from Corning Life Sciences (Tewksbury, MA, USA). Cannulated Sprague-Dawley (SD) rats were purchased from Harlan Laboratories (Indianapolis, IN, USA); blank rat plasma was obtained from Biomedical (Winchester, VA, USA). Hard gelatin capsules (size 1) were purchased from Capsugel Inc., Morristown, NJ, USA, and all other reagents used are of analytical grade.

Nekkanti V., Wang Z, & Betageri G.V. (2016). Pharmacokinetic Evaluation of Improved Oral Bioavailability of Valsartan: Proliposomes Versus Self-Nanoemulsifying Drug Delivery System. AAPS PharmSciTech, 17(4).

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Paclitaxel-Loaded Lipid Nanocarriers

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Tricaprylin was kindly supplied by Croda Health Care (Edison, NJ, USA). Soy phosphatidylcholine (PC) and 2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (DPPE) were obtained from Avanti Polar Lipids (Alabaster, AL, USA), and propylene glycol and glycerol were purchased from Synth (São Paulo, SP, Brazil). Paclitaxel and elacridar were obtained from Cayman Chemical Company (Ann Arbor, MI, USA). Tributyrin (Tri), perillyl alcohol (PA), tetrazolium dye 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), and polysorbate 80 were purchased from Sigma (St Louis, MO, USA). Other specific reagents are described along with the respective methodology. Ultrapure water was employed unless stated.

Salata G.C, & Lopes L.B. (2022). Phosphatidylcholine-Based Nanoemulsions for Paclitaxel and a P-Glycoprotein Inhibitor Delivery and Breast Cancer Intraductal Treatment. Pharmaceuticals, 15(9), 1110.

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Preparation and Characterization of Nanoparticles

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Rap (purity >99%) was kindly provided by Chong Kun Dang Pharm. Co. (Yongin, Korea). Soy phosphatidylcholine (SPC; purity >99%) and distearoylphosphatidylethanolamine-polyethylene glycol₂₀₀₀-folate (DSPE-PEG₂₀₀₀-Fol; DP_2KF) were purchased from Avanti^® Polar Lipids (Alabaster, AL, USA). 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI), phosphate buffered saline (PBS) tablets, and cholesterol were purchased from Sigma-Aldrich (St. Louis, MO, USA). Folic acid was purchased from Duksan Pure Chemical Co., Ltd. (Seoul, Korea). Acetonitrile, chloroform, dimethylsulfoxide, and other solvents purchased from commercial sources were of analytical or cell culture grade. The following antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA): rabbit polyclonal antibodies against phosphorylated AMPKα (Thr172), phosphorylated mTOR (Ser2448), phosphorylated 4EBP-1, phosphorylated p70S6 (Ser371), phosphorylated ULK1 (Ser555 and Ser757), and cleaved PARP. Mouse monoclonal antibody against β-actin was purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Rabbit polyclonal antibodies against LC3B, p62, and beclin-1 were purchased from Abcam (Cambridge, MA, USA).

Yoon H.Y., Chang I.H., Goo Y.T., Kim C.H., Kang T.H., Kim S.Y., Lee S.J., Song S.H., Whang Y.M, & Choi Y.W. (2019). Intravesical delivery of rapamycin via folate-modified liposomes dispersed in thermo-reversible hydrogel. International Journal of Nanomedicine, 14, 6249-6268.

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Comprehensive Inflammatory Pathway Analysis

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All used reagents were from Sigma-Aldrich Co., Saint Louis, MO, USA. Soy phosphatidylcholine was from Avanti Polar Lipids, Inc. (Alabaster, Alabama, USA). For Western blot (WB) analysis, the following antibodies were used: mouse anti-superoxide dismutase 2 (SOD2) (1:400, sc-1331340), mouse anti-TNFα (1:400, sc-52746), mouse anti-IL-1β (1:400, sc-52012), mouse anti-β-actin (1:4000, sc-47778) and rabbit anti-mouse IgG H&L (HRP) (1:10000, ab6728), which were from Santa Cruz Biotechnology, Dallas, TX, USA and Abcam, Cambridge, UK. The foetal calf serum (FCS) was from Euroclone, Pero, Milan, Italy, EU, the 100 kDa cutoff Amicon centrifugal filter columns were from Millipore (Billerica, MA, USA), Trizol was from Ambion, (ThermoFisher Scientific, Waltham, MA, USA), primers were from Biolegio BV (Nijmegen, Netherland) and M-MLV Reverse Transcriptase and SyBr Select Master Mix were from Invitrogen (ThermoFisher Scientific, Waltham, MA, USA). The HPLC-grade solvents were from Merck (Kenilworth, NJ, USA).

Deleanu M., Toma L., Sanda G.M., Barbălată T., Niculescu L.Ş., Sima A.V., Deleanu C., Săcărescu L., Suciu A., Alexandru G., Crişan I., Popescu M, & Stancu C.S. (2023). Formulation of Phytosomes with Extracts of Ginger Rhizomes and Rosehips with Improved Bioavailability, Antioxidant and Anti-Inflammatory Effects In Vivo. Pharmaceutics, 15(4), 1066.

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Liposomal Doxorubicin Formulation and Characterization

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Egg phosphatidylcholine (EPC), soy phosphatidylcholine (SPC), hydrogenated soy phosphatidylcholine (HSPC), 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 1,2-disteoroyl-sn-glycero-3-phosphatidylcholine (DSPC), and 1,2-distearoyl-snglycerophosphoethanolamine poly(ethylene glycol)₂₀₀₀ (DSPE-PEG-2000) were obtained from Avanti Polar Lipids, Inc. (Alabaster, AL). Cholesterol and barbituric acid were purchased from Sigma-Aldrich (St. Louis, MO). Liposomes were formed by the lipid film hydration method as described [28 (link), 31 (link)]. In brief, 25 mg of lipid mixture dissolved in chloroform was dried, and the resultant thin film was hydrated using 1 ml BA at 20 mg/ml and pH 7.3 to form multilamellar vesicles. The mixture was then annealed at 55–65 °C according to the type of PCs, sonicated, and subsequently extruded through stacked polycarbonate filters. DOX at 2 mg/ml was remotely loaded via sonication after extrusion. Liposomes were then filtered through Sephadex G-50 gel columns (GE Healthcare Life Sciences, Pittsburg, PA) to remove unloaded compounds, and stored at 4°C prior to use. The size (z-average) and heterogeneity in size (polydispersity index, PDI) were measured in PBS at room temperature by dynamic light scattering using a Nanosizer ZS90 (Malvern Instruments, Southborough, MA).

Chan K.W., Yu T., Qiao Y., Liu Q., Yang M., Patel H., Liu G., Kinzler K.W., Vogelstein B., Bulte J.W., van Zijl P.C., Hanes J., Zhou S, & McMahon M.T. (2014). A diaCEST MRI approach for monitoring liposomal accumulation in tumors. Journal of controlled release : official journal of the Controlled Release Society, 180, 51-59.

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