Ovarian carcinoma A2780 and paclitaxel-resistant A2780 (A2780/Taxol) ovarian carcinoma cell lines were purchased from Nanjing KeyGen Biotech Co., Ltd. (Nanjing, China). Cells were maintained in DMEM (A2780) or RPMI-1640 with 800 ng/ml paclitaxel (A2780/Taxol) supplemented with 10% FBS, 100 U/ml penicillin and 100 µg/ml streptomycin. The cells were cultured in a humidified atmosphere containing 5% CO2 at 37°C.
Paclitaxel
Manufactured by Keygen Biotech
Sourced in China
Paclitaxel is a chemotherapy medication used to treat various types of cancer. It is a complex organic compound extracted from the bark of the Pacific yew tree. Paclitaxel functions by disrupting the normal division of cells, which is an essential process for the growth and spread of cancer cells.
Automatically generated - may contain errors
2 protocols using paclitaxel
1
Culturing Ovarian Cancer Cell Lines
2
Dual-Drug Nano-Carrier for Ovarian Cancer
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paclitaxel (PTX, purity ≥98%) and tetrandrine (TET, purity ≥98%) were purchased from Melonepharma Co., Ltd. (Dalian, China). MAL-PEG-DSPE (1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[maleimide(polyethylene glycol)-2000]) was purchased from Avanti Polar Lipids, Inc. (Alabaster, USA). PLGA (D,L-lactide-co-glycolide, MW 5000, lactide: glycolide (50:50)), dicyclohexylcarbodiimide (DCC), 4-dimethylaminopyridine (DMAP), 3,3′-dithiodipropionic acid (DTPA), egg lecithin (EPC), cholesterol (CHOL), MTT and Hoechst 33342 were obtained from Sigma-Aldrich (St. Louis, MO). iRGD peptide (sequence: CRGDK/RGPD/EC, purity >95%) with a residual thiol group was custom-synthesized by GL Biochem Co. Ltd. (Shanghai, China). All other chemicals were of analytical grade and were used as received.
The sensitive human ovarian carcinoma A2780 and paclitaxel-resistant A2780/PTX cell lines were obtained from KeyGen Biotech Co., Ltd. (Nanjing, China) and were maintained in DMEM (Life Technologies Co.) containing 10% fetal bovine serum (FBS, Gibco), 2 mM L-glutamine, penicillin-streptomycin solution (40 U/mL each, Gibco, Life Technologies Co.) at 37 °C in a humidified CO2 (5%) incubator.
The sensitive human ovarian carcinoma A2780 and paclitaxel-resistant A2780/PTX cell lines were obtained from KeyGen Biotech Co., Ltd. (Nanjing, China) and were maintained in DMEM (Life Technologies Co.) containing 10% fetal bovine serum (FBS, Gibco), 2 mM L-glutamine, penicillin-streptomycin solution (40 U/mL each, Gibco, Life Technologies Co.) at 37 °C in a humidified CO2 (5%) incubator.
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