Quercetin

Manufactured by Sisco Research Laboratories

Sourced in India

Quercetin is a naturally occurring plant-based compound. It functions as an antioxidant, which helps neutralize harmful free radicals in the body.

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7 protocols using quercetin

Quercetin-Based Compound Characterization

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The compound Quercetin (QUA) [2-(3,4-dihydroxyphenyl)-3,5,7-trihydroxychromen-4-one], with a purity of 98%, extra pure grade yellow color, and a CAS number of 6151-25-3, was acquired from Sisco Research Laboratories Pvt. Ltd. (Mumbai, India). Copper sulfate pentahydrate (CuSO₄·5H₂O) and Tween 80 were obtained from Merck (Mumbai, India). The reference/standard drugs Ondansetron (OND), Hyoscine butyl bromide (HYS), and Domperidone (DOM) were obtained from Incepta Pharma. Ltd. (Dhaka, Bangladesh), Opsonin Pharma. Ltd. (Barishal, Bangladesh), and Beximco Pharma. Ltd. (Dhaka, Bangladesh), respectively, all located in Bangladesh.

Chowdhury R., Bhuia M.S., Rakib A.I., Hasan R., Coutinho H.D., Araújo I.M., de Menezes I.R, & Islam M.T. (2023). Assessment of Quercetin Antiemetic Properties: In Vivo and In Silico Investigations on Receptor Binding Affinity and Synergistic Effects. Plants, 12(24), 4189.

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Quantifying Flavonoid Content in Extracts

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The total flavonoid content was determined using the Dowd method as adapted by Arvouet-Grand [26 (link)] and reported by others [16 (link),27 ,28 (link)]. Briefly, 2 mL of 2% aluminium trichloride (AlCl₃) (Changzhou Baixing, Tianjin, China) in methanol was mixed with equal volume of the extract and incubated at room temperature for 10 min before reading the absorbance at 415 nm against a blank sample consisting of 2 mL of extract and 2 mL of methanol without AlCl₃. The total flavonoid content was determined using a standard curve with quercetin (Sisco Research Laboratories, Mumbai, India) as the standard. The mean of three readings was expressed as milligrams of quercetin equivalent per gram of the weight of extract (mg QE/gDW).

Saka S.O., Salisu Y.Y., Sahabi H.M., Sanusi K.O., Ibrahim K.G., Abubakar M.B., Isa S.A., Liman M.G., Shehu S., Malami I., Chan K.W., Azmi N.H, & Imam M.U. (2023). Nutrigenomic Effects of White Rice and Brown Rice on the Pathogenesis of Metabolic Disorders in a Fruit Fly Model. Molecules, 28(2), 532.

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Evaluation of Bioactive Compounds in Samara White Wine

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The wine samples were purchased from the local market. The brand was -Samara White Wine (Alcohol content: 11% v/v) manufactured by Sula Vineyards Pvt. Ltd., Nashik, Maharashtra, India. The natural components-Bromelain, Quercetin, Ascorbic acid and Histamine (standard) were purchased from Sisco Research Laboratories Pvt Ltd with other consumables.

Kalhaa R.K., Damgude G., & Dabade A. (2021). Reduction of food histamine in white wine by Bromelain, Quercetin and Ascorbic acid. Food Science and Applied Biotechnology, 4(2), 130-130.

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Antioxidant Evaluation of Natural Compounds

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2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′- azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS), 6-hydroxy-2,5,7,8-tetramethyl chroman-2-carboxylic acid (Trolox), Folin–Ciocalteu reagent, gallic acid, sodium carbonate, aluminum chloride, potassium acetate, quercetin, acetic acid, hexane, metaphosphoric acid, and 2,6-dichlorophenol indophenol sodium salt (all chemicals of AR grade) were procured from Sisco Research Laboratories Pvt. Ltd. (SRL), Mumbai, India. Throughout the experiment, deionized water that had been filtered through a 0.22 μm filter was used.

Saini R., Kaur S., Aggarwal P, & Dhiman A. (2023). The influence of conventional and novel blanching methods on potato granules, phytochemicals, and thermal properties of colored varieties. Frontiers in Nutrition, 10, 1178797.

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Antioxidant Capacity Determination Protocol

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Folin-Ciocalteu reagent and quercetin were obtained from Qualigens, Mumbai, India. Ascorbic acid, gallic acid, quercetin, L-Ascorbic acid, potassium thiocyanate, ethylene diamine tetra acetic acid (EDTA), 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2-deoxyribose, thiobarbituric acid (TBA), sodium nitroprusside, N-(1-Naphthyl) ethylenediamine dihydrochloride, potassium hexacyanoferrate (K₃Fe (CN)₆), trichloroacetic acid (TCA), ferric chloride were procured from SRL Chemicals, India. All the other reagents and solvents were of analytical grade.

Eshwarappa R.S., Iyer R.S., Subbaramaiah S.R., Richard S.A, & Dhananjaya B.L. (2014). Antioxidant activity of Syzygium cumini leaf gall extracts. BioImpacts : BI, 4(2), 101-107.

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Antioxidant Capacity Evaluation Protocol

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Folin-Ciocalteu (FC) reagent and quercetin were obtained from Qualigens, Mumbai, India. Ascorbic acid, Gallic acid, quercetin, L-Ascorbic acid, potassium thiocyanate, ethylene diamine tetra acetic acid (EDTA), 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2-deoxyribose, thiobarbituric acid (TBA), sodium nitroprusside, N-(1-Naphthyl) ethylenediamine dihydrochloride, potassium hexacyanoferrate (K₃Fe (CN)₆), trichloroacetic acid (TCA) and ferric chloride were procured from SRL Chemicals, India. All the other reagents and solvents were of analytical grade.

Eshwarappa R.S., Iyer S., Subaramaihha S.R., Richard S.A, & Dhananjaya B.L. (2015). Antioxidant activities of ficus glomerata (moraceae) leaf gall extracts. Pharmacognosy Research, 7(1), 114-120.

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Antioxidant Activity Assay Protocol

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Folin-Ciocalteu reagent and quercetin were obtained from Qualigens, Mumbai, India. Ascorbic acid, Gallic acid, quercetin, L-Ascorbic acid, potassium thiocyanate, ethylene diamine tetra acetic acid (EDTA), 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2-deoxyribose, thiobarbituric acid (TBA), sodium nitroprusside, N-(1-Naphthyl) ethylenediamine dihydrochloride, potassium hexacyanoferrate (K 3 Fe (CN) 6 ), trichloroacetic acid (TCA), ferric chloride were procured from SRL Chemicals, India. All the other reagents and solvents were of analytical grade.

Eshwarappa R.S., Lakshmikantha R.Y., Subaramaihha S.R., Subbaiah S.G., Surendranath A.R, & Dhananjaya B.L. (2015). Antioxidant activity of insect gall extracts of Pistacia integerrima. Acta scientiarum polonorum. Technologia alimentaria, 14(4).

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