One week prior to experiments, rats were acclimated to metabolism cages (Nalgene Supply, Rochester, NY) and to harnesses (Instech Laboratories, Plymouth Meeting, PA) for six hr/day. The rat’s femoral vein catheter was used for drug administration and the jugular vein catheter was used for blood collection. On the first day of the experiment, the infusion harnesses were connected to a tether that protected two tubing extensions. The drug administration extension (0.023-in inner diameter and 0.038-in outer diameter; Intramedic® Polyethylene Tubing, Becton Dickinson, Parsippany, NJ) was attached to the rat’s femoral vein catheter. The blood withdrawal extension was attached to the rat’s jugular vein catheter. The rat was then placed in the metabolic cage and the tether was attached to a swivel. This allowed free movement in the cage during drug administration and blood sampling. The METH solution (0.2 mg/ml) was dispensed from a 500 μl dead volume syringe (Hamilton Company, Reno, NV) via a polyethylene extension attached to a programmable Harvard infusion pump (Harvard Apparatus, Holliston, MA).
Harvard infusion pump
Manufactured by Harvard Apparatus
Sourced in United States
The Harvard infusion pump is a versatile laboratory instrument designed for precise fluid delivery. It is capable of accurately controlling the flow rate and volume of liquid infusions. The pump utilizes an adjustable syringe mechanism to deliver fluids at a range of flow rates, making it suitable for a variety of applications in life science research and clinical settings.
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Lab products found in correlation
3 protocols using harvard infusion pump
1
Rat Acclimation and Drug Administration Protocol
2
Microdialysis of Glutamate and GABA in Rat SNc
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Conventional microdialysis experiments were carried out in SNc to determine basal dialysate levels of glutamate and GABA. Adult rats were anesthetized with urethane 1.5 g/kg i.p. and placed in a stereotaxic apparatus, the skull was exposed, and a hole was drilled. Then, a concentric microdialysis probe (CMA 11 Microdialysis, Holliston, MA) with an in vitro recovery rate higher than 10% was lowered diagonally into the SNc using a 40° angle from the horizontal axis at the following coordinates: −4.9 AP, +7.6 ML relative to bregma, and −8.0 DV from the dura (Paxinos and Watson, 2009 ). Body temperature was maintained by a thermostatically controlled electric heating pad. The probe was perfused for 40 minutes with KRP buffer at a rate of 2 µL/min using a Harvard infusion pump (Harvard Apparatus). After a stabilization period using KRP, 3 consecutive samples were collected every 5 minutes for the determination of GABA and glutamate basal dialysate. Perfusion samples were collected in 2 µL perchloric acid (0.2 N) and maintained on ice (4°C) until quantification.
3
Neonicotinoid Residue Detection via UHPLC-MS/MS
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A Dionex Ultimate 3000 RS UHPLC + focused system coupled with a TSQ Altis triple quadrupole mass spectrometer (MS/MS, Thermo Fisher Scientific, Austin, TX, USA) was used to detect residues of the seven neonicotinoids. Trace Finder software (version 4.1) was used for analysis, data acquisition, and reporting. An Accucore RP-MS C18 column (150 × 2.1 mm, 2.6 μm film thickness, Thermo Fisher Scientific) was used for separation at 40 °C. Mobile phase A was water, and mobile phase B was methanol; the flow rate was 0.30 mL/min. The gradient program of the mobile phase was 0–2 min 10% B, 2–6 min from 10% B to 90% B, 6–8 min 90% B, 8–8.1 min from 90% B to 10% B, and 8.1–16 min 10% B. The injection volume was 2 μL. To optimize the MS/MS parameters of the tested analytes (Table S1 ), a Harvard infusion pump (Harvard Apparatus, South Natick, MA, USA) was used. The precursor ions [M + H]+ were identified in the multiple reaction monitoring (MRM) mode using an electrospray ionization interface in the positive ion mode (H-ESI+). The MS conditions were as follows: the ion source temperature was set at 325 °C, the ion spray voltage was set at 3800 V, and the sheath and auxiliary gasses were 40 and 10 arb, respectively. Trace Finder (version 4.2) software was used for data acquisition.
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