Cell cycle distribution of SMYD3 knockdown and scramble PCa cells was determined by flow cytometry. Briefly, 5 × 105 harvested cells were fixed overnight at 4°C;C with 70% cold ethanol. After washing with cold PBS, cells were ressuspended in staining Propidium Iodide Solution (Cytognos S.L, Salamanca, Spain) and incubated for 30 minutes at room temperature. All cells were then measured on a Cytomics FC500 flow cytometer (Beckman Coulter, Fullerton, CA, USA) and analyzed using Modfit LT (Verity Software House, Inc, Topshan, Maine, USA). Three biological replicates were performed for sh-scramble and sh-SMYD3.
Propidium iodide solution
Manufactured by Cytognos
Sourced in Spain
Propidium Iodide Solution is a fluorescent dye used in flow cytometry and other laboratory techniques. It binds to DNA and intercalates between the base pairs, allowing the quantification of cellular DNA content.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using propidium iodide solution
1
Cell Cycle Analysis of SMYD3 Knockdown
2
Cell Cycle Analysis of Prostate Cancer Cells
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Cell cycle distribution of LNCaP and PC3 cells was determined by flow cytometry. Briefly, 72 h after transfections, 5 × 105 harvested cells were fixed overnight at 4 °C with 70% cold ethanol. After cold PBS washing, cells were re-suspended in staining Propidium Iodide Solution (Cytognos S.L, Salamanca, Spain) and incubated for 30 min at room temperature. All cells were then measured on a Cytomics FC500 flow cytometer (Beckman Coulter, Fullerton, CA, USA) and analyzed using Modfit LT (Verity Software House, Inc., Topshan, Maine, USA).
3
Flow Cytometric Cell Cycle Analysis of PCa Cells
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Cell cycle distribution of PCa cells was determined by flow cytometry. Briefly: 5×105 harvested cells were fixed overnight at 4ºC with 70% cold ethanol. After washing with cold PBS, cells were ressuspended in staining Propidium Iodide Solution (Cytognos S.L, Salamanca, Spain) and incubated for 30 minutes at room temperature. All cells were then measured on a Cytomics FC500 flow cytometer (Beckman Coulter, Fullerton, CA, USA) and analyzed using Modfit LT (Verity Software House, Inc, Topshan, Maine, USA).
4
Flow Cytometry Analysis of PC3 Cell Cycle
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Cell cycle distribution of PC3 cells was determined by flow cytometry. Briefly, 72 h after transfection (150,000 cells/well at day 0, in 6-well plates), 5 × 105 harvested cells were fixed overnight at 4 °C with 70% cold ethanol. After washing with cold PBS, cells were re-suspended in Propidium Iodide Solution (Cytognos S.L, Salamanca, Spain) and incubated for 30 min at room temperature. All cells were then measured on a Cytomics FC500 flow cytometer (Beckman Coulter, Fullerton, CA, USA) and analysed using Modfit LT (Verity Software House, Inc., Topshan, ME, USA).
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