Etoac

All the chemicals, reagents and the solvents used in the assay protocols were of analytical grade. Ascorbic acid, sodium phosphate, phosphate buffer saline, ammonium molybdate, sodium citrate, DPPH, DMSO, Folin-Ciocalteu reagent, sodium acetate trihydrate, sodium hydroxide, and sodium citrate were obtained from Sigma Aldrich, USA. Other chemicals such as gallic acid, TRIS-HCl, sodium carbonate, sodium chloride, potassium ferricyanide, ferric chloride, tripyridyl-s-triazine (TPTZ), potassium dihydrogen phosphate, disodium hydrogen phosphate, and metaphosphoric acid were purchased from Sigma Aldrich. n-Hexane, EtOAc, MeOH, C₂H₅OH, H₂SO₄ of analytical grade and other chemicals were procured from Daejung Chemicals and Metals (Seoul, South Korea). Column chromatography was conducted by using silica gel (70–230 mesh) and LiChroprep RP-18 (40–63 μm; ODS silica gel) from Merck. Thin layer chromatography was achieved on glass backed precoated silica gel 60 F₂₅₄ plates (Merck). Resolution of the spots in TLC plates was checked by dipping the plate in a solution of 5% vanillin-H₂SO₄ in C₂H₅OH. Standards were procured from Sigma-Aldrich, USA. Instrumentation details such as optical rotation, FT-IR, ESI/FT mass and NMR spectra were given in the literature (Chung et al., 2014b (link)).