Fmoc l ala oh

2-chlorotrityl chloride resin (100–200 mesh; 1% DVB; 1.01 mmol/g), Fmoc-L-Phe-OH, Fmoc-Gly-OH and Fmoc-L-Ala-OH were purchased from Shanghai GL biochem (China). DMEM, α-MEM medium and Phosphate Buffered Saline (PBS, 0.0067 M) were provided by Thermo Fisher Scientific Corporation (USA). Fetal bovine serum (FBS, Gibco, South America origin) was bought from Life Technologies Corporation (USA). All the other compounds were obtained by Chengdu Best-reagent Corporation (China) and used without further purification. ¹H NMR spectra were collected on a Bruker AVII-400 (400 MHz, USA) using DMSO‑d₆ as a solvent. Liquid chromatography-mass spectrometry (LC-MS) analysis was performed on a Finnigan TSQ Quantum Ultra LC-MS system with an electrospray ionization (ESI) source.

All chemicals were purchased from commercial vendors and used without further purification. Fmoc-L-Gly-OH, Fmoc-L-Leu-OH, Fmoc-L-Ile-OH, Fmoc-L-Val-OH, Fmoc-L-Lys(Boc)-OH, Fmoc-L-Lys(Alloc)-OH, Fmoc-L-Ala-OH, Fmoc-L-Cys(Trt)-OH, Fmoc-L-Gln(Trt)-OH, Fmoc-L-Asn(Trt)-OH, Fmoc-L-Glu(OtBu)-OH, Fmoc-L-Asp(OtBu)-OH, Fmoc-L-Arg(Pbf)-OH, Fmoc-L-Phe-OH, Fmoc-L-Trp(Boc)-OH, Fmoc-His(Boc)-OH, Fmoc-L-Ser(tBu)-OH, Fmoc-L-Thr(tBu)-OH, Fmoc-L-Tyr(tBu)-OH, Fmoc-L-Pro-OH, Boc-L-Asp(OtBu)-OH, and Fmoc-L-Met-OH were purchased from GL Biochem. Fmoc-Rink amide resin was purchased from Tjhecheng. Bromoacetic acid, [2-[2-(Fmoc-amino)ethoxy]ethoxy]acetic acid, N,N′-diisopropylcarbodiimide (DIC), ethyl cyanoglyoxylate-2-oxime (Oxyma), N,N-dimethylformamide (DMF), dichloromethane (DCM), diethyl ether, trifluoroacetic acid, triisoproprylsilane, 4-methyl pepiridine and HPLC-grade acetonitrile were obtained from Energy Chemical.