Lf sa8001

Chemicals of the LW1564 series were synthesized and dissolved in 20 mM DMSO stock solution. Sodium pyruvate, sodium malate, sodium succinate, L-ascorbic acid, N,N,N,N-tetramethyl-p-phenylenediamine, rotenone, antimycin A, KCN and Nile red were purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). HypoFluor MAR was purchased from GORYO Chemical, Inc. (Sapporo, Japan), and calcein AM was purchased from AnaSpec, Inc. (San Jose, CA, USA). Primary antibodies against the following proteins were used: HIF-1α (#610958, BD Biosciences, San Jose, CA, USA), CyclinD1 (#554180, BD Biosciences), β-tubulin (ab-15568, Abcam, Cambridge, Cambridgeshire, UK), β-actin (sc-47778, Santa Cruz, CA, USA), acetyl-CoA carboxylase-α (ACCα) (sc-30212, Santa Cruz), sterol regulatory element-binding protein (SREBP)-1 (sc-366, Santa Cruz), AMPKα (sc-25792, Santa Cruz), P-AMPKα (#2531, Cell Signaling, MA, USA), P-ACC (#3661, Cell Signaling), mTOR (#2983, Cell Signaling), P-mTOR (#2971, Cell Signaling), eukaryotic translation initiation factor 4E binding protein-1 (4EBP1) (#9644, Cell Signaling) and P-4EBP1 (#9459, Cell Signaling). A horseradish peroxidase-conjugated anti-mouse (LF-SA8001, AbFrontier, Seoul, Korea) or anti-rabbit secondary antibody (LF-SA8002, AbFrontier) was used.