Total RNA of cells was extracted using the miRNeasy Mini Kit (QIAGEN) according to the manufacturer’s instructions. After confirming the purity and quantity of each sample using an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) and an RNA 6000 Nano kit (Agilent Technologies), respectively, the samples were labeled using a miRCURY Hy3 Power Labeling kit (Exiqon A/S, Vedbaek, Denmark) and hybridized to a human miRNA Oligo Chip (v.21; Toray Industries, Inc., Tokyo, Japan). Chips were scanned using a 3D-Gene Scanner 3000 (Toray Industries). The 3D-Gene extraction software version 1.2 (Toray Industries) was used to calculate the raw signal intensity of the images. The raw data were analyzed using GeneSpring GX 10.0 software (Agilent Technologies) to assess the differences in miRNA expression between the samples. Global normalization was performed on raw data obtained above the background level. Differentially expressed miRNAs were determined using Welch’s t-test.
Oligo chip v 21
Manufactured by Toray
Sourced in Japan
The Oligo Chip (v.21) is a lab equipment product developed by Toray. It is designed for the analysis and identification of oligonucleotide sequences. The product's core function is to provide a platform for the hybridization and detection of specific oligonucleotide targets.
Automatically generated - may contain errors
Lab products found in correlation
3d gene extraction software version 1, by Toray (2 mentions)
Genespring gx 10, by Agilent Technologies (2 mentions)
3d gene scanner 3000, by Toray (2 mentions)
Agilent 2100 bioanalyzer, by Agilent Technologies (2 mentions)
Mirneasy mini kit, by Qiagen (2 mentions)
Rna 6000 nano kit, by Agilent Technologies (2 mentions)
2 protocols using oligo chip v 21
1
Profiling Human miRNA Expression
2
Aspirin Modulates miRNA Expression
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cells were treated with 2.5 mmol/l aspirin for 48 h, and total RNA was extracted using the miRNeasy Mini kit (Qiagen GmbH) according to the manufacturer's instructions. After confirming the purity and quantity of each sample using an Agilent 2100 Bioanalyzer and an RNA 6000 Nano kit (both from Agilent Technologies), respectively, the samples were labeled using a miRCURY Hy3 Power Labeling kit (Exiqon A/S) and hybridized to a human miRNA Oligo Chip (v.21; Toray Industries, Inc.). Chips were scanned using the 3D-Gene Scanner 3000 (Toray Industries, Inc.). The 3D-Gene extraction software version 1.2 (Toray Industries, Inc.) was used to calculate the raw signal intensity of the images. The raw data were analyzed using the GeneSpring GX 10.0 software (Agilent Technologies, Inc.) to assess the differences in miRNA expression between the aspirin-treated and control samples. Global normalization was performed on raw data obtained above the background level. Differentially expressed miRNAs were determined using Welch's t-test.
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