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Anti jak3

Manufactured by Cell Signaling Technology
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Anti-JAK3 is a primary antibody that specifically recognizes the Janus Kinase 3 (JAK3) protein. JAK3 is a non-receptor tyrosine kinase that plays a critical role in the JAK-STAT signaling pathway, which is involved in various cellular processes, including immune function and cell growth. The Anti-JAK3 antibody can be used to detect and study the expression and localization of JAK3 in biological samples.

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Lab products found in correlation

Anti jak1, by Cell Signaling Technology (3 mentions) Anti stat3, by Cell Signaling Technology (3 mentions) Anti stat1, by Cell Signaling Technology (2 mentions) Anti socs3, by Cell Signaling Technology (2 mentions) Anti socs1, by Cell Signaling Technology (2 mentions) Anti jak2, by Cell Signaling Technology (2 mentions) Anti gapdh, by Cell Signaling Technology (2 mentions) Anti p stat1 y701, by Cell Signaling Technology (1 mentions) Anti mecp2, by Cell Signaling Technology (1 mentions) Anti socs5, by Cell Signaling Technology (1 mentions) Anti pstat3 y705, by Cell Signaling Technology (1 mentions) Odyssey system, by LI COR (1 mentions) Anti β actin, by Merck Group (1 mentions) Anti phospho p38 mapk, by Cell Signaling Technology (1 mentions) Anti irf6, by Cell Signaling Technology (1 mentions) Anti blnk, by Cell Signaling Technology (1 mentions) Anti α tubulin, by Cell Signaling Technology (1 mentions) Anti p38 mapk, by Cell Signaling Technology (1 mentions) Anti phospho jnk, by Cell Signaling Technology (1 mentions) Anti jnk, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

Anti-phospho-JAK3 (Tyr980/981), (2 citations) Anti-Phospho-JAK3 (2 citations)

6 protocols using anti jak3

1

Western Blotting and Flow Cytometry Protein Detection

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The following primary antibodies were used for detecting proteins by Western blotting analysis or by intracellular staining and flow cytometry: anti-STAT1, anti-STAT3, anti-pSTAT1 (Y701), anti-pSTAT3 (Y705), anti-SOCS1, anti-SOCS3, anti-SOCS5, anti-JAK1, anti-JAK2, anti-JAK3, anti-MeCP2 (all from Cell Signaling Technology), and anti-β-actin (Sigma). The primary anti-SOCS5 antibody used to analyze LLO118 TCR transgenic T cells was purchased from Santa Cruz Biotechnology. Alexa Fluor 680–conjugated anti-rabbit antibody and Alexa Fluor 800–conjugated anti-goat antibody (Invitrogen) were used as secondary antibodies, and fluorescence intensity was measured on an Odyssey system (Licor).
Jiang S., Li C., McRae G., Lykken E., Sevilla J., Liu S.Q., Wan Y, & Li Q.J. (2014). MeCP2 Reinforces STAT3 Signaling and the Generation of Effector CD4+ T Cells by Promoting miR-124–Mediated Suppression of SOCS5. Science signaling, 7(316), ra25.
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2

Antibody Panel for Cellular Signaling

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Anti-IRF6 (cat# 6948 S), anti-BLNK (cat# 36438), anti-p38MAPK (cat# 9212 S), anti-phospho-p38MAPK (cat# 4511 T), anti-caspase-3 (cat# 9962 S), anti-JNK (cat# 9255 S), anti-phospho-JNK (cat# 9252 S), anti-Jak3 (cat# 5481) and anti-α-tubulin (cat# 3873) were from Cell Signalling Technology, Danvers, MA, USA.
Liu X., Chipurupalli S., Jiang P., Tavasoli M., Yoo B.H., McPhee M., Mazinani S., Francia G., Kerbel R.S, & Rosen K.V. (2022). ErbB2/Her2-dependent downregulation of a cell death-promoting protein BLNK in breast cancer cells is required for 3D breast tumor growth. Cell Death & Disease, 13(8), 687.
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3

Immunoblotting Analysis of Phosphorylated Signaling Proteins

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Affinity-purified F(ab′)2 fragments of polyclonal goat anti-mouse IgM (anti-Ig), and HRP-conjugated goat anti-rabbit secondary antibodies, were obtained from Jackson ImmunoResearch Laboratories. Anti-phospho-PKCδ (pTyr311), anti-PKCδ, anti-phospho-Lyn (pTyr507), anti-Lyn, anti-phospho-Fyn (pTyr530), anti-Fyn, anti-phospho-Blk (pTyr389), anti-Blk, anti-phospho-Syk (pTyr352), anti-Syk, anti-phospho-Src (pTyr416), anti-Src anti-JAK1, anti-JAK3, anti-GAPDH, and anti-actin Abs for immunoblotting were obtained from Cell Signaling Technology. Anti-phospho-Lyn (pTyr396) was obtained from Abcam. Protein A/G ultralink resin was obtained from Thermo Scientific. LY294002, was obtained from Calbiochem. PP2 and PP3 were obtained from abcam. SU6656 was obtained from Santa Cruz Biotechnology. Recombinant murine IL-4 and goat anti-IL-4Rα antibody was obtained from R&D Biosystems. HRP-conjugated rabbit anti-goat secondary antibody, was obtained from southern biotech.
Khan N., Hu Y., Lowell C.A, & Rothstein T.L. (2021). Signal integration by translocation and phosphorylation of PKCδ in the B cell alternate pathway. Journal of immunology (Baltimore, Md. : 1950), 207(9), 2288-2296.
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4

Immunoblotting for PDGF-stimulated Signaling

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Antibodies used were anti-Abi1 (1:1000, Sigma #A5106-200UL, L/N 076M4842V), anti-GAPDH (1: 1000, Santa Cruz Biotechnology #sc-32233, K0315), anti-JAK2 (Santa Cruz Biotechnology #sc-294, G1414 and Thermo# AHO1352, QC216934), anti-phospho-Jak2 (Y1007/Y1008) (Cell Signaling, #3771S/10), anti-phospho-Jak1 (Y1034/Y1035) (Cell Signaling, # 74129S/2), anti-Jak1 (Cell Signaling, #50996), anti-phospho-Jak3 (Y980/Y981) (Cell Signaling, # 5031S/7), anti-Jak3 (Cell Signaling, #5481), anti-phospho-Tyk2 ((Y1054/Y1055) (Cell Signaling, # 68790S/1), anti-Tyk2 (SC-5271), anti-phospho-STAT1 (Y701) (Cell Signaling, #9167S/25), anti-STAT1 (Cell Signaling, #9176S), anti-phospho-STAT2 (Y960) (Cell Signaling, # 88410S/4), anti-STAT2 (Cell Signaling, #4597S), anti-phospho-Stat3 (Y705) (Cell Signaling, #9145/1), anti-Stat3 (Invitrogen, #MA1-13042/PJ208446), anti-phospho-PDGFRβ (Tyr751) (Cell Signaling, #3161/7) and anti-PDGFRβ (Proteintech, #13449-1-AP/# 00070807). The antibodies were validated by examining the molecular weight of target proteins. In addition, anti-Abi1 was validated by using Abi1 KD cells. Finally, vendors have provided datasheet to show that antibodies were validated by positive controls. For protein phosphorylation experiments, cells were treated with 10 ng/mL PDGF for 10 min followed by immunoblot analysis (see below).
Wang R., Wang Y., Liao G., Chen B., Panettieri RA J.r., Penn R.B, & Tang D.D. (2022). Abi1 mediates airway smooth muscle cell proliferation and airway remodeling via Jak2/STAT3 signaling. iScience, 25(2), 103833.
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5

Immunoblotting Analysis of Signaling Pathways

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The cells were lysed with NP-40 lysis buffer and centrifuged at 15,000 rpm for 15 min at 4 °C. The supernatants were collected, and total protein content was measured using the Bradford assay (Bio-Rad Laboratories). Lysates were separated with Bolt 4 to 12 % Bis-Tris polyacrylamide gels (Thermo Fisher Scientific), transferred to membrane filters, and subjected to immunoblotting using anti-GP130 (1:2000, Cell Signaling, #3732), anti-LIFR (1:1000, Santa Cruz Biotechnology, A-10), anti-EGFR (1:1000, Cell Signaling, #2232), anti-p-EGFR-Tyr1068 (1:1000, Cell Signaling, #2234), anti-AKT (1:1000, Cell Signaling, #4691), anti-p-AKT-Ser473 (1:1000, Cell Signaling, #4060), anti-ERK1/2 (1:1000, Cell Signaling, #9102), anti-p-ERK1/2-Thr202/Tyr204 (1:1000, Cell Signaling, #9101), anti-MEK1/2 (1:1000, Cell Signaling, #8727), anti-p-MEK1/2-Ser217/221 (1:1000, Cell Signaling, #9154), anti-Stat3 (1:1000, Cell Signaling, #4904), anti-p-Stat3-Tyr705 (1:1000, Cell Signaling, #9145), anti-Jak1 (1:1000, Cell Signaling, #3344), anti-p-Jak1-Tyr1034/1035 (1:1000, Cell Signaling, #74129), anti-Jak2 (1:1000, Cell Signaling, #3230), anti-p-Jak2-Tyr1008 (1:1000, Cell Signaling, #8082), anti-Jak3 (1:1000, Cell Signaling, #8827), anti-p-Jak3-Tyr980/981 (1:1000, Cell Signaling, #5031), and anti-beta-tubulin mouse antibody (1:5000, Sigma).
Hara T., Chanoch-Myers R., Mathewson N.D., Myskiw C., Atta L., Bussema L., Eichhorn S.W., Greenwald A.C., Kinker G.S., Rodman C., Castro L.N., Wakimoto H., Rozenblatt-Rosen O., Zhuang X., Fan J., Hunter T., Verma I.M., Wucherpfennig K.W., Regev A., Suvà M.L, & Tirosh I. (2021). Interactions between cancer cells and immune cells drive transitions to mesenchymal-like states in glioblastoma. Cancer cell, 39(6), 779-792.e11.
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6

Immunoblotting analysis of JAK-STAT signaling

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ZT55 was synthesized by the Chinese Academy of Medical Sciences and Peking Union Medical College (CAMS & PUMC, Beijing, China). Anti-phospho-JAK1 (Y1022/1023), anti-JAK1, anti-phospho-JAK2 (Y1007/1008), anti-JAK2, anti-phospho-JAK3 (Tyr980/981), anti-JAK3, anti-phospho-STAT5 (Tyr694), anti-STAT5, anti-phospho-STAT3 (Tyr705), anti-STAT3, anti-Bcl-2, anti-Bax, anti-SOCS1, anti-SOCS3 and anti-GAPDH antibodies were purchased from Cell Signaling Technology (CST, Danvers, MA, USA). Recombinant human JAK1, JAK2, and JAK3 were purchased from Thermo Fisher Scientific (Waltham, Massachusetts, USA).
Hu M., Xu C., Yang C., Zuo H., Chen C., Zhang D., Shi G., Wang W., Shi J, & Zhang T. (2019). Discovery and evaluation of ZT55, a novel highly-selective tyrosine kinase inhibitor of JAK2V617F against myeloproliferative neoplasms. Journal of Experimental & Clinical Cancer Research : CR, 38, 49.
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