Q tracker 800 cell labeling kit

Human adipose-derived MSCs were purchased from Invitrogen (Carlsbad, CA, USA). MSCs were cultured in Mesen PRO RS Basal Medium supplemented with Mesen PRO RS growth supplement (Invitrogen) and incubated at 37℃ in a 5% CO₂ atmosphere. Cells were used after 2-5 passages. MSCs were labeled using the QDs (Q-Tracker 800) Cell Labeling Kit (Invitrogen). MSCs (1×10⁶) were suspended with 200 µL of complete growth medium and labeled with 10 nM of QDs labeling solution. After 60 minutes, labeled cells with QDs were washed twice with complete growth medium.

For injected pioWJMSC tracking in the lung, we performed fluorescence optical imaging analysis, as described previously24 . In brief, pioWJMSCs were labeled using the quantum dots (QD) (Q-Tracker 800) Cell Labeling Kit (Invitrogen, Carlsbad, CA, USA). PioWJMSCs (1×10⁶) were suspended in 200 µL of complete growth medium and labeled with 10 nM of QDs labeling solution. After 60 minutes, QD-labeled cells were washed twice with complete growth medium. We suspended 3×10⁵QDs 800-labeled pioWJMSCs in 100 µL saline and injected into the mice through the tail vain. The mice were sacrificed after 1, 4, 24, and 72 hours and image of the lung was taken using the IVIS Spectrum Pre-clinical In vivo Imaging System (PerkinElmer, Waltham, MA, USA).