Vero cells were seeded in a confocal dish (Solarbio) and transfected with EGFP-PARP12 and DsRed-NS1 or NS3 plasmids. After 24 hours, cells were fixed with 4% paraformaldehyde for 15 min and permeabilized in 0.2% Triton X-100 for 15 min at room temperature. The cells were washed three times with PBS. Nuclei were stained with 4′,6-diamidino-2-phenylindole (Thermo Fisher Scientific). Cells were imaged on an LSM 700 (Carl Zeiss) confocal microscope, and the images were analyzed with Imaris and ImageJ software.
Confocal dish
Manufactured by Solarbio
The Confocal dish is a specialized laboratory equipment designed for imaging and analysis of biological samples. It provides a controlled environment for cell culture and observation under a microscope. The dish features a transparent glass or plastic bottom that allows for high-resolution imaging of the specimen.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using confocal dish
1
Visualization of PARP12 and Viral Proteins
2
Visualization of PARP11 and PARP12 in Vero Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Vero cells were seeded in a confocal dish (Solarbio) and transfected with EGFP-PARP12 and DsRed-PARP11 plasmids. After 24 h, cells were fixed with 0.4% paraformaldehyde for 15 min and permeabilized in 0.2% Triton-X100 for 15 min at room temperature. The cells were washed three times with PBS supplemented with 0.05% Tween-20. Nuclei were stained with 4′6-diamidino-2-phenylindole (Thermo Fisher Scientific). Cells were imaged on a LSM700 (Carl Zeiss) confocal microscope, and the images were analyzed with ImageJ software.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!