GC-MS analyses of neem seed extracts were performed on a Clarus 600 GC (Perkin Elmer, USA) System, fitted with a non-polar capillary column, coupled to a Clarus 600 C MS. Helium was used as the carrier gas at a constant flow rate of 1.0 mL/min [51 (link)]. The injection and ion source temperatures were 270 and 230ºC, respectively. The ionizing energy was set at 70 eV. Oven temperature was programmed from 60ºC (hold for 2 min) to 260ºC at a rate of 3 ºC/min. The crude samples were diluted with methanol (1:100, v/v) and filtered, particle-free dilute seed extracts were taken in a syringe and injected into the injector with a split ratio of 20:1. The mass spectra were computer matched with those of standards available in mass spectrum libraries (National Institute of Standards and Technology (NIST) Mass Spectral Library).
Clarus 600 gc
Manufactured by PerkinElmer
Sourced in United States
The Clarus 600 GC is a gas chromatograph designed for analytical applications. It is capable of separating and identifying a wide range of chemical compounds. The core function of the Clarus 600 GC is to provide accurate and reliable analysis of complex samples.
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2 protocols using clarus 600 gc
1
GC-MS Analysis of Neem Seed Extracts
2
Polychlorinated Phenolics Analysis by GC-MS
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The polychlorinated phenolics and derivative products were analysed using a gas chromatography (GC) system comprising of a clarus 600 GC, clarus 600T mass spectrometer (MS), attached to a turbomatrix 40 trap headspace sampler (PerkinElmer, South Africa division). The chemical separation component was the Elite 5MS GC system capillary column (30 m, 250 µm) from PerkinElmer. Helium (He) carrier gas of 99.999% purity and applied at a flow rate of 1 mL/min. MS interface comprised of an Electron Ioniser (EI) and a high performance mass analyser.
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