All solvents used in this study—acetonitrile, dichloromethane, ethyl acetate, methanol, n-hexane—were of analytical grade and purchased from Merck (Darmstadt, Germany). The deuterated solvents—water-D2, methanol-D4, and 3-(trimethylsilyl)-1-propanesulfonic acid sodium salt (TSP)—were purchased from Armar AG (Döttingen, Switzerland) and ultrapure water was produced using the Milli-Q Simplicity 185 system (Millipore Corp., Billerica, MA, USA). Formic acid (FA), MS-grade, was purchased from Sigma-Aldrich (St. Louis, MO, USA). The LiChroprep RP-18, Silica gel 60 (60–200 mesh) was purchased from Merck-Sigma-Aldrich (Steinheim, Germany).
Milli q simplicity 185 system
Manufactured by Merck Group
Sourced in United States
The Milli-Q Simplicity 185 system is a water purification system designed to produce ultrapure water. It utilizes a multi-stage purification process to remove contaminants and produce water with a high degree of purity and low levels of organic and inorganic impurities.
Automatically generated - may contain errors
4 protocols using milli q simplicity 185 system
1
Analytical-Grade Solvent Preparation
2
Analytical Grade Solvents Protocol
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All solvents (methanol and acetonitrile) used for this work were of analytical grade and purchased from Merck (Darmstadt, Germany). Ultrapure water was obtained from the Milli-Q® Simplicity 185 system (Millipore Corp., Billerica MA). MS-grade formic acid (FA) and KCl were also used.
3
Amino Acid Analysis by HPLC
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HPLC-grade acetonitrile, phenyl isothiocyanate (PITC), orto-phosphoric acid, sodium acetate, DL-Methionine sulfone were purchased from Sigma-Aldrich (Steinheim, Germany). Ultrapure water used for the preparation of all reagents, eluents, and buffers was obtained from a Milli-Q-simplicity 185 system (Millipore, Bedford, MA, USA). All solutions and reagents were filtered through 0.45 µm MS® Nylon membrane filters. The amino acid standards alanine (Ala), aspartic acid (Asp), arginine (Arg), glutamic acid (Glu), glycine (Gly), hydroxyproline (Hyp); histidine (His), isoleucine (Ile), leucine (Leu), lysine (Lys), methionine (Met), phenylalanine (Phe), proline (Pro), serine (Ser),, tyrosine (Tyr), threonine (Thr), tryptophan (Trp) and valine (Val) were from Sigma-Aldrich (Steinheim, Germany).
4
Peanut Allergen Isolation and Quantification
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All buffer reagents were supplied by Sigma-Aldrich (The Netherlands). All solutions were prepared using deionised water purified with a Milli-Q Simplicity 185 system (Millipore).
Natural Ara h 1, extracted from light roasted peanut flour (purity < 95%), and monoclonal anti-Ara h 1 antibody 2C12 were purchased from Indoor Biotechnologies Limited (UK).
Carboxyl acid-coated superparamagnetic particles of 2.8 μm diameter (Dynabeads TM M-270 Carboxylic Acid) were acquired from Thermo Fisher Scientific (Norway).
Natural Ara h 1, extracted from light roasted peanut flour (purity < 95%), and monoclonal anti-Ara h 1 antibody 2C12 were purchased from Indoor Biotechnologies Limited (UK).
Carboxyl acid-coated superparamagnetic particles of 2.8 μm diameter (Dynabeads TM M-270 Carboxylic Acid) were acquired from Thermo Fisher Scientific (Norway).
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