As 1111

We fixed the cells by incubating in 4% PFA solution (AS1018, Aspen, Wuhan) for 30 min. Then, after blocking the fixed cells with 10% BSA (10735078001, Roche) at 37 °C for 30 min, they were incubated at 4 °C overnight with the following primary antibodies: anti-SERPINH1 (ab109117, Abcam); anti-E-cadherin (20874-1-AP, San Ying, Wuhan); and anti-N-cadherin (Abcam, Ab18203). Then, the cells were incubated with CY3- or FITC-conjugated goat anti–mouse or goat anti–rabbit IgG (AS-1111, Aspen, Wuhan) at 37 °C for 1 h. The cells were imaged and analyzed under a fluorescence microscope.

The cells were fixed with 4% paraformaldehyde (AS1018, Aspen, Wuhan) for 20 min. Then, the fixed cells were blocked with 10% bovine serum albumin (AS1018, Aspen, Wuhan) for 30 min, followed by incubation at 4°C overnight with the following primary antibodies: anti-E-cadherin (20874-1-AP, San Ying, Wuhan), anti-N-cadherin (Abcam, Ab18203), and anti-β-catenin (20874-1-AP, San Ying, Wuhan). After incubating the cells with CY3- or FITC-conjugated goat anti–mouse or goat anti–rabbit IgG (AS-1111, Aspen, Wuhan) secondary antibody at 37°C for 40 min, they were captured using fluorescence microscopy.