Rabbit anti myc ab9106

HeLa Cells were plated onto glass coverslips and transfected with TDP-4FL using Fugene HD (Promega) for 24 Hours. Cells were fixed with 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA, USA), permeabilized with 0.1% Triton-X (Thermo Fisher Scientific), and blocked with 2% FBS 1× DPBS. Primary antibodies were diluted in 2% FBS in 1× DPBS. Cells were stained with 300nM of DAPI, and coverslips were mounted onto glass slides using ProLong Glass Antifade Mountant (Thermo Fisher Scientific). Confocal images were obtained using a Leica TCS SPE laser scanning confocal microscope. Primary antibodies used include mouse anti-Myc (9E10), rabbit anti-Myc (ab9106, Abcam), mouse anti-VCP (NB120–11433, Novus Biologicals), and mouse anti-ubiquitin (ST1200, Millipore, Burlington, MA, USA) with Alexa Flour 488 or 568 (Invitrogen) as secondary antibodies.