Lentiviruses containing miR-20a-5p mimics (miR-20a-5p), miR-20a-5p sponge (anti-miR-20a-5p), and negative control of miRNA (miR-NC) with green fluorescent protein (GFP) were purchased from Hanbio. Cell transfection with miR-20a-5p, anti-miR-20a-5p, and NC at a multiplicity of infection (MOI) of 50 was implemented with 5 mg/ml polybrene (Invitrogen). The hDPSCs were exposed to viral supernatant for 24 h, followed by treated with 1 μg/ml puromycin (Sigma). Then, the cells were washed with phosphate-buffered saline (PBS) and cultured in a general medium. The proportion of GFP-positive cells was observed with a microscope (IX71; Olympus, Tokyo, Japan) to calculate transfection efficiency.
The RNA oligoribonucleotides including the small interfering RNAs (siRNAs) targeting bone morphogenetic protein and activin membrane-bound inhibitor (BAMBI) (si-BAMBI) and NC were designed and synthesized by GenePharma. The hDPSCs (2×105 per well) were seeded on 24-well plates and cultured with DMEM, and they were transfected using Lipofectamine 3000 Reagent (Invitrogen) after 70% confluency. The primers of these RNA oligoribonucleotides were listed in TableS1 .
The RNA oligoribonucleotides including the small interfering RNAs (siRNAs) targeting bone morphogenetic protein and activin membrane-bound inhibitor (BAMBI) (si-BAMBI) and NC were designed and synthesized by GenePharma. The hDPSCs (2×105 per well) were seeded on 24-well plates and cultured with DMEM, and they were transfected using Lipofectamine 3000 Reagent (Invitrogen) after 70% confluency. The primers of these RNA oligoribonucleotides were listed in Table