Ab139418 propidium iodide flow cytometry kit

Manufactured by BD

The Ab139418 propidium iodide flow cytometry kit is a laboratory reagent used for DNA content analysis and cell cycle studies. The kit contains propidium iodide, a fluorescent dye that binds to DNA, allowing for the detection and quantification of DNA content in cells using flow cytometry technology.

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Lab products found in correlation

Epics xl mcl flow cytometer, by Beckman Coulter (2 mentions) Epics xlmcl flow cytometer equipment, by Beckman Coulter (1 mentions)

Spelling variants of this product

Flow cytometry (2933 citations) Propidium iodide (1522 citations) Propidium Iodide flow cytometry kit (2 citations) Propidium iodide flow cytometry (2 citations)

4 protocols using ab139418 propidium iodide flow cytometry kit

Apoptosis Detection by Flow Cytometry

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All adherent
and detached cells were collected, harvested, and then washed two
times with PBS. An Annexin V, FITC (fluorescein isothiocyanate) conjugate/propidium
iodide (PI) apoptosis detection kit was used according to the manufacturer’s
procedure (ab139418 propidium iodide flow cytometry kit/BD). After
adding the Annexin V-FITC conjugate into the labeled tube for 5 min,
a PI stain was also added and the tube was incubated for 10 min in
the dark at 37 °C. Samples were investigated with scan flow cytometry
(FAC), and the data were evaluated using the CellQuest analysis software
program (Becton–Dickinson, USA).^{32 (link),33 (link)}

Fazary A.E., Alfaifi M.Y., Elbehairi S.E., Amer M.E., Nasr M.S., Abuamara T.M., Badr D.A., Ju Y.H, & Mohamed A.F. (2021). Bioactivity Studies of Hesperidin and XAV939. ACS Omega, 6(30), 20042-20052.

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Cell Cycle Analysis of Compound 15 in HepG2 Cells

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Flow cytometry analysis was carried out using an ab139418 Propidium Iodide flow cytometry kit/BD to detect the effect of the synthesized compound 15 on cell cycle distribution. First, HepG2 cell were seeded in 6-well plates at a density of 2 × 10⁵/well and incubated for 24 h. HepG2 cells were then treated with 9.61 µM of compound 15 for 24 h. The treated cells were then fixed in 70% ethanol for 12 h at 4 °C, rinsed with cold PBS, incubated with 100 μL RNase A for 0.5 h at 37 °C, and stained with Propidium Iodide (400 μL) in the dark at RT for an extra 0.5 h. The stained cells were determined utilizing Epics XLMCL™ flow cytometer equipment (Beckman Coulter, Apeldoorn, Netherlands), and the results were collected and analyzed using Flowing software (version 2.5.1, Turku Centre for Biotechnology, Turku, Finland).

Alanazi A.S., Mirgany T.O., Alsfouk A.A., Alsaif N.A, & Alanazi M.M. (2023). Antiproliferative Activity, Multikinase Inhibition, Apoptosis- Inducing Effects and Molecular Docking of Novel Isatin–Purine Hybrids. Medicina, 59(3), 610.

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Cell Cycle Analysis of HepG-2 Cells

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Cell cycle flow cytometry was performed using propidium iodide (PI) staining (ab139418_Propidium Iodide Flow Cytometry Kit/BD)⁶⁶ (link). IC₅₀ of both 4 and 4NPs was introduced to HepG-2 cells and then incubated for 48 h. The cells were then fixed in 70% ethanol for 12 h at 4 °C. After that, the cells were washed with cold PBS, incubated with 100 μL RNase A at 37 °C for 30 min, and stained with 400 μL PI for another 30 min at room temperature in the dark. Flowing software was used to analyse the data after measuring the stained cells with an Epics XL-MCL™ Flow Cytometer (Beckman Coulter).

El-Kalyoubi S., El-Sebaey S.A., El-Sayed A.A., Abdelhamid M.S., Agili F, & Elfeky S.M. (2023). Novel pyrimidine Schiff bases and their selenium-containing nanoparticles as dual inhibitors of CDK1 and tubulin polymerase: design, synthesis, anti-proliferative evaluation, and molecular modelling. Journal of Enzyme Inhibition and Medicinal Chemistry, 38(1), 2232125.

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Cell Cycle Analysis of Compound 5d in HepG2 Cells

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Flow cytometry analysis was conducted to assess the effect of compound 5d on the cell cycle distribution in HepG2 cells. The Flow Cytometry Kit for cell cycle analysis (ab139418_Propidium Iodide Flow Cytometry Kit/BD) was used in this test. First, the HepG2 cells were seeded in six-well plates at a density of 2 × 10⁵ cells/well and incubated for 24 h. In the second step, 10% FBS was added, and the cells were incubated at 37 °C and 5% CO₂. The medium was replaced with (DMSO 1% v/v) containing 7.1 µM of compound 5d and then incubated for 24 h. After that, the cells were fixed with 70% ethanol at 4 °C for 12 h and washed with cold PBS, and then rinsed with PBS. In the last step, 100 μL of RNase A was added to each well and incubated at 37 °C for 30 min and stained with 400 μL PI in the dark at room temperature for an additional 30 min. The stained cells were measured using an Epics XL-MCL™ Flow Cytometer (Beckman Coulter), and the data were analyzed using Flowing software (version 2.5.1, Turku Centre for Biotechnology, Turku, Finland).

Altharawi A., Alanazi M.M., Alossaimi M.A., Alanazi A.S., Alqahtani S.M., Geesi M.H, & Riadi Y. (2023). Novel 2-Sulfanylquinazolin-4(3H)-one Derivatives as Multi-Kinase Inhibitors and Apoptosis Inducers: A Synthesis, Biological Evaluation, and Molecular Docking Study. Molecules, 28(14), 5548.

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