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Np28 ficoll

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NP28-Ficoll is a laboratory product used for the separation and purification of cells and cellular components. It is a density gradient medium composed of sucrose-epichlorohydrin copolymers. NP28-Ficoll can be used to isolate specific cell types or subcellular fractions based on their density differences.

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Lab products found in correlation

Recombinant baff, by R&D Systems (1 mentions) Automacs, by Miltenyi Biotec (1 mentions)

3 protocols using np28 ficoll

1

NP-Ficoll Immunization and Antibody Quantification

Cited 1 time
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Mice were immunized i.p. as indicated with 5 μg NP (4-Hydroxy-3-nitrophenylacetic) hapten conjugated to Amino-Ethyl-Carboxy-Methyl-FICOLL (NP28-Ficoll; Biosearch Technologies). Serum was obtained at day 0 before immunization and 7 days later and NP-specific (allotype-specific) IgM ELISAs and ELISpots performed as described previously (32 (link)).
Hu J., Oda S.K., Shotts K., Donovan E.E., Strauch P., Pujanauski L.M., Victorino F., Al-Shami A., Fujiwara Y., Tigyi G., Oravecz T., Pelanda R, & Torres R.M. (2014). Lysophosphatidic Acid (LPA) Receptor 5 Inhibits B Cell Antigen Receptor Signaling and Antibody Response. Journal of immunology (Baltimore, Md. : 1950), 193(1), 85-95.
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2

Investigating B cell activation and differentiation

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Single cell suspensions were prepared from the spleens of 8 week old C57BL/6-IghB1-8/B1-8 mice after red blood cell lysis and B cells purified using negative selection with anti-CD43 magnetic microbeads and an autoMACS (Miltenyi Biotec). B cell purity as determined by flow cytometric analysis was at all times >95%. B cells were resuspended at 2 × 106 cells per ml and stimulated with 10 ng/ml of NP28-Ficoll (Biosearch Technologies) in the presence of 10 ng/ml recombinant BAFF (R&D Systems) to promote B cell survival and with or without 20 μM LPA or 50 μM sterile-filtered OTP. After 24 hours, cells were collected, stained, and analyzed for the expression of Igλ, CD86 and CD69 by flow cytometry.
Hu J., Oda S.K., Shotts K., Donovan E.E., Strauch P., Pujanauski L.M., Victorino F., Al-Shami A., Fujiwara Y., Tigyi G., Oravecz T., Pelanda R, & Torres R.M. (2014). Lysophosphatidic Acid (LPA) Receptor 5 Inhibits B Cell Antigen Receptor Signaling and Antibody Response. Journal of immunology (Baltimore, Md. : 1950), 193(1), 85-95.
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3

NP-Specific Antibody Response Protocol

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Mice were immunized intraperitoneally (i.p.) with 40 μg of NP-ovalbumin (NP-OVA, Biosearch Technology) precipitated with Complete Freund’s Adjuvant (Difco) or Alum. For eliciting TI antigen responses, mice were i.p. immunized with 20 μg of NP28-Ficoll (Biosearch Technologies) or with 10 μg NP-LPS. Mice were bled on day 9 (for T-dependent antigen) or day 7 (for T-independent antigen) post-immunization. NP-specific IgG and IgM titers were determined by ELISA after serial dilutions of the serum.
Wang N., Halibozek P.J., Yigit B., Zhao H., O’Keeffe M.S., Sage P., Sharpe A, & Terhorst C. (2015). Negative Regulation of Humoral Immunity Due to Interplay between the SLAMF1, SLAMF5, and SLAMF6 Receptors. Frontiers in Immunology, 6, 158.
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