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Polymer refine kit for dab

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The Leica Polymer Refine Kit for DAB is a laboratory equipment product designed for immunohistochemical staining. It provides the necessary components to enhance the visualization of specific target antigens in tissue samples using the DAB (3,3'-Diaminobenzidine) chromogen.

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Lab products found in correlation

Edta based solution, by Leica (2 mentions) Dako anti mouse hrp, by Agilent Technologies (2 mentions) Bond 3, by Leica (2 mentions)

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Leica refine DAB kit (2 citations) Refine DAB (2 citations) Leica Refine Kit (1 citations)

2 protocols using polymer refine kit for dab

1

Immunohistochemical Evaluation of BRAF V600E and Beta-Catenin

Check if the same lab product or an alternative is used in the 5 most similar protocols
We performed immunohistochemical studies on five-micrometer-thick whole tissue sections of formalin-fixed, paraffin-embedded tissue in a Bond 3 automated immunostainer (Leica Microsystems, Bannockburn, IL, USA) using a primary antibody against BRAF V600E (clone: VE1, 1:100, Spring Bioscience, Pleasanton, CA) and a primary antibody against beta-catenin. For BRAF V600E, we deparaffinized the sections on the Leica Bond using Bond Dewax solution and performed antigen retrieval with an EDTA-based solution (Leica) at pH 9 and Leica Polymer Refine Kit for DAB (Diaminobenzidine) staining. Appropriate positive and negative controls were included. Positive staining was characterized by diffuse and moderate cytoplasmic staining of the tumor cells. We considered isolated nuclear staining, weak staining of occasional cells, or faint diffuse staining as negative staining. For beta-catenin (BD pharmigen, cat# 610154, mouse-monoclonal, clone: 14), antigen retrieval was performed in a pressure cooker in citrate buffer (pH=6.0, 1:1000 dilution) with a 45 minute incubation followed by Dako anti-mouse-HRP for 30 minutes at room temperature. Cases with nuclear staining (which ranged from low level to high level) were scored as positive and membranous staining were scored as negative.
Brastianos P.K., Taylor-Weiner A., Manley P.E., Jones R.T., Dias-Santagata D., Thorner A.R., Rodriguez F.J., Bernardo L.A., Schubert L., Sunkavalli A., Shillingford N., Calicchio M.L., Lidov H.G., Taha H., Martinez-Lage M., Santi M., Storm P.B., Lee J.Y., Palmer J.N., Adappa N.D., Scott R.M., Dunn I.F., Laws ER J.r., Stewart C., Ligon K.L., Hoang M.P., Van Hummelen P., Hahn W.C., Louis D.N., Resnick A.C., Kieran M.W., Getz G, & Santagata S. (2014). Exome sequencing identifies BRAF mutations in papillary craniopharyngiomas. Nature genetics, 46(2), 161-165.
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2

Immunohistochemical Evaluation of BRAF V600E and Beta-Catenin

Check if the same lab product or an alternative is used in the 5 most similar protocols
We performed immunohistochemical studies on five-micrometer-thick whole tissue sections of formalin-fixed, paraffin-embedded tissue in a Bond 3 automated immunostainer (Leica Microsystems, Bannockburn, IL, USA) using a primary antibody against BRAF V600E (clone: VE1, 1:100, Spring Bioscience, Pleasanton, CA) and a primary antibody against beta-catenin. For BRAF V600E, we deparaffinized the sections on the Leica Bond using Bond Dewax solution and performed antigen retrieval with an EDTA-based solution (Leica) at pH 9 and Leica Polymer Refine Kit for DAB (Diaminobenzidine) staining. Appropriate positive and negative controls were included. Positive staining was characterized by diffuse and moderate cytoplasmic staining of the tumor cells. We considered isolated nuclear staining, weak staining of occasional cells, or faint diffuse staining as negative staining. For beta-catenin (BD pharmigen, cat# 610154, mouse-monoclonal, clone: 14), antigen retrieval was performed in a pressure cooker in citrate buffer (pH=6.0, 1:1000 dilution) with a 45 minute incubation followed by Dako anti-mouse-HRP for 30 minutes at room temperature. Cases with nuclear staining (which ranged from low level to high level) were scored as positive and membranous staining were scored as negative.
Brastianos P.K., Taylor-Weiner A., Manley P.E., Jones R.T., Dias-Santagata D., Thorner A.R., Rodriguez F.J., Bernardo L.A., Schubert L., Sunkavalli A., Shillingford N., Calicchio M.L., Lidov H.G., Taha H., Martinez-Lage M., Santi M., Storm P.B., Lee J.Y., Palmer J.N., Adappa N.D., Scott R.M., Dunn I.F., Laws ER J.r., Stewart C., Ligon K.L., Hoang M.P., Van Hummelen P., Hahn W.C., Louis D.N., Resnick A.C., Kieran M.W., Getz G, & Santagata S. (2014). Exome sequencing identifies BRAF mutations in papillary craniopharyngiomas. Nature genetics, 46(2), 161-165.
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