Tumor sections were evaluated for PD-L1 expression by immunohistochemistry (IHC). Harvested tumors were fixed in 10% neutral buffered formalin, embedded in paraffin, and 4 μm thick sections were obtained on slides. After deparaffinizing with xylene and alcohol gradients, antigen retrieval was done using 10 mM citrate buffer, pH 6.0 (#S1699, Dako target retrieval solution). Tumor sections were then treated with 3% H2O2 for 10 minutes, blocked with 5% goat serum for 1 h, and then incubated with a primary anti-human PD-L1 antibody (#13684, Cell Signaling) at 1:500 dilution at 4°C overnight. Subsequently, using Dako CSAII Biotin-free Tyramide Signal Amplification System kit, slides were incubated with secondary antibody, amplification reagent, and with anti-fluorecein-HRP. Finally, staining was carried out by adding DAB chromogen. Sections were counterstained with hematoxylin, followed by dehydration with alcohol gradients, xylene washes and mounted with a cover slip.
Primary anti human pd l1 antibody
Manufactured by Cell Signaling Technology
The primary anti-human PD-L1 antibody is a laboratory reagent used to detect the presence and expression of the PD-L1 protein in human samples. It is a tool for researchers to study the role of PD-L1 in various biological processes and disease states.
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4 protocols using primary anti human pd l1 antibody
1
Immunohistochemical Assessment of PD-L1 Expression in Tumors
2
Immunohistochemical Evaluation of PD-L1
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Tumor sections were evaluated for PD-L1 expression by IHC as reported previously (11 (link)). Briefly, 4–5-μm tumor sections were treated with 3% H2O2 for 10 minutes, blocked with 5% goat serum for 1 hour, and then incubated with a primary anti-human PD-L1 antibody (#13684, Cell Signaling Technology) at 1:500 dilution at 4°C overnight. Subsequently, using Dako CSAII Biotin-free Tyramide Signal Amplification System Kit, slides were incubated with secondary antibody and amplification reagent. Final staining was carried out by adding 3,3'-Diaminobenzidine.
3
PD-L1 Expression Evaluation by IHC
4
Immunohistochemical Analysis of PD-L1 Expression
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Tumor sections
were evaluated
for PD-L1expression by immunohistochemistry (IHC). Harvested tumors
were fixed in 10% neutral buffered formalin and embedded in paraffin,
and 4 μm thick sections were obtained on slides. After deparaffinizing
with xylene and alcohol gradients, antigen retrieval was done using
10 mM citrate buffer, pH 6.0 (#S1699, Dako target retrieval solution).
Tumor sections were then treated with 3% H2O2 for 10 min, blocked with 5% goat serum for 1 h, and then incubated
with a primary antihuman PD-L1 antibody (#13684, Cell Signaling) at
1:500 dilution at 4 °C overnight. Subsequently, using Dako CSAII
Biotinfree Tyramide Signal Amplification System kit, slides were incubated
with secondary antibody, amplification reagent and with antifluorecein-HRP.
Finally, staining was carried out by adding DAB chromogen. Sections
were counterstained with hematoxylin, followed by dehydration with
alcohol gradients, xylene washes, and mounted with a coverslip.
were evaluated
for PD-L1expression by immunohistochemistry (IHC). Harvested tumors
were fixed in 10% neutral buffered formalin and embedded in paraffin,
and 4 μm thick sections were obtained on slides. After deparaffinizing
with xylene and alcohol gradients, antigen retrieval was done using
10 mM citrate buffer, pH 6.0 (#S1699, Dako target retrieval solution).
Tumor sections were then treated with 3% H2O2 for 10 min, blocked with 5% goat serum for 1 h, and then incubated
with a primary antihuman PD-L1 antibody (#13684, Cell Signaling) at
1:500 dilution at 4 °C overnight. Subsequently, using Dako CSAII
Biotinfree Tyramide Signal Amplification System kit, slides were incubated
with secondary antibody, amplification reagent and with antifluorecein-HRP.
Finally, staining was carried out by adding DAB chromogen. Sections
were counterstained with hematoxylin, followed by dehydration with
alcohol gradients, xylene washes, and mounted with a coverslip.
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