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Sodium dodecyl sulfate (sds)

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Sodium dodecyl sulfate (SDS) is an anionic surfactant commonly used in laboratory settings. It functions as a detergent, solubilizing and denaturing proteins by disrupting non-covalent interactions within and between them.

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Lab products found in correlation

Perchloric acid, by Thermo Fisher Scientific (2 mentions) Medetomidine, by Henry Schein (2 mentions) Sodium deoxycholate, by Merck Group (2 mentions) Methanol, by Thermo Fisher Scientific (2 mentions) Trichostatin a, by Merck Group (2 mentions) Halt protease and phosphatase inhibitor cocktail, by Thermo Fisher Scientific (2 mentions) 4 2 hydroxyethyl piperazine 1 ethanesulfonic acid hepes, by Merck Group (2 mentions) Asp n, by Roche (2 mentions) Thiamet g, by Bio-Techne (2 mentions) Phosphatase inhibitor cocktails 1 or 3, by Merck Group (2 mentions) Dimethylamino phenyl n hydroxy 4 6 dimethyl 7 oxo 2 4 heptadienamide, by Merck Group (2 mentions) Niacinamide pyridine 3 carboxylic acid amide, by Merck Group (2 mentions) Proteomic grade trypsin, by Promega (2 mentions) Triton x 100, by Merck Group (2 mentions) Complete mini, by Roche (2 mentions)

2 protocols using sodium dodecyl sulfate (sds)

1

Protein Extraction and Modification Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Phosphatase inhibitor cocktails 1 or 3, and 2 (100x, Sigma) and protease inhibitor tablets (cOmplete, EDTA-free and cOmplete-mini, EDTA-free; Roche) were used interchangeably with Halt protease and phosphatase inhibitor cocktails (Thermo Scientific); all were used at 1x according to the manufacturers’ instructions. Thiamet G (4390, Tocris) was used in a range of concentrations, as specified. Trichostatin A ([R-(E,E)]-7-[4-(dimethylamino)phenyl]-N-hydroxy-4,6-dimethyl-7-oxo-2,4-heptadienamide, Sigma) was used at 3 μM and niacinamide (pyridine-3-carboxylic acid amide, Sigma) at 10 mM. Other reagents were ketamine HCl (Bionichepharma), medetomidine (Dexdomitor, Henry Schein), perchloric acid (Fisher), methanol (Fisher), 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES, Sigma), sodium chloride (5M, Cellgrow), sodium dodecyl sulfate (from 10% solution, Teknova), TritonX-100 (Sigma), and sodium deoxycholate (Sigma). All reagents for peptide digestions were from Sigma, except proteomic-grade trypsin (Promega) and AspN (Roche).
Morris M., Knudsen G.M., Maeda S., Trinidad J.C., Ioanoviciu A., Burlingame A.L, & Mucke L. (2015). Tau post-translational modifications in wildtype and human amyloid precursor protein transgenic mice. Nature neuroscience, 18(8), 1183-1189.
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2

Protein Extraction and Modification Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Phosphatase inhibitor cocktails 1 or 3, and 2 (100x, Sigma) and protease inhibitor tablets (cOmplete, EDTA-free and cOmplete-mini, EDTA-free; Roche) were used interchangeably with Halt protease and phosphatase inhibitor cocktails (Thermo Scientific); all were used at 1x according to the manufacturers’ instructions. Thiamet G (4390, Tocris) was used in a range of concentrations, as specified. Trichostatin A ([R-(E,E)]-7-[4-(dimethylamino)phenyl]-N-hydroxy-4,6-dimethyl-7-oxo-2,4-heptadienamide, Sigma) was used at 3 μM and niacinamide (pyridine-3-carboxylic acid amide, Sigma) at 10 mM. Other reagents were ketamine HCl (Bionichepharma), medetomidine (Dexdomitor, Henry Schein), perchloric acid (Fisher), methanol (Fisher), 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES, Sigma), sodium chloride (5M, Cellgrow), sodium dodecyl sulfate (from 10% solution, Teknova), TritonX-100 (Sigma), and sodium deoxycholate (Sigma). All reagents for peptide digestions were from Sigma, except proteomic-grade trypsin (Promega) and AspN (Roche).
Morris M., Knudsen G.M., Maeda S., Trinidad J.C., Ioanoviciu A., Burlingame A.L, & Mucke L. (2015). Tau post-translational modifications in wildtype and human amyloid precursor protein transgenic mice. Nature neuroscience, 18(8), 1183-1189.
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