293-AR or LNCaP cells were treated with drugs for 24–72hrs and then lysed in TBS with 0.1% Tween with protease inhibitors. Lysates were applied for immunoblot assays or immunoprecipitation with anti-AR antibody (Santa Cruz Biotechnology, cat no. 7305). Western blot was used to detect AR, casein kinase 1 alpha (EnCor, CPCA-CK1a), β-catenin or phosphor-β-catenin (Cell Signaling, cat no.9562 and 9564), or GAPDH (Santa Cruz, cat no. 47724) as a loading control.
Phosphor β catenin
Manufactured by Cell Signaling Technology
Phosphor-β-catenin is a laboratory reagent used to detect and quantify the phosphorylated form of the β-catenin protein. β-catenin is a key component of the Wnt signaling pathway and its phosphorylation status is an important indicator of cellular signaling events.
Automatically generated - may contain errors
2 protocols using phosphor β catenin
1
AR and β-catenin Regulation Assay
2
Quantitative Analysis of Ocular Neovascularization
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Whole eyes from the above-mentioned mice were perfusion fixed with 4% paraformaldehyde (PFA) and extracted for cryosection into 10 μm sections. Sections were stained with Alexa Fluor 488 conjugated isolectin (GS-IB 4) (Catalog # I21411, ThermoFisher Scientific) for neovascularization and primary antibodies against F4/80 (Invitrogen) for macrophage infiltration, as well as other proteins of interest including HTRA1, VEGF, phosphor-β-catenin (Cell Signaling Tech), which were detected with a fluorescent-conjugated secondary antibody of different wavelength to examine the location and quantity of these proteins. Nuclei were stained with 4',6-diamidino-2-phenylindole (DAPI). We visualized stain co-localization via the surface plot for pixel density generated by ImageJ.
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