Alanine aminotransferase alt kit

Manufactured by Nanjing Jiancheng

Sourced in China

The Alanine aminotransferase (ALT) kit is a laboratory reagent used to measure the activity of the enzyme alanine aminotransferase in biological samples. ALT is an enzyme found primarily in the liver and is commonly used as a biomarker for liver function.

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Alanine aminotransferase (57 citations) Alanine aminotransferase (ALT) (15 citations) ALT Kit (9 citations) Alanine aminotransferase (ALT) assay kit (8 citations)

5 protocols using alanine aminotransferase alt kit

Hepatoprotective Effects of H. candicans

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H candicans was purchased from Ganlu Traditional Medicine (Tibet, China) and was identified by the Shaanxi Chinese Medicine Institute. Bifendate (C₂₀H₁₈O₁₀, DDB) (Cat#H44023176) was purchased from Baiyunshan Xingqun Pharmaceutical Co., Ltd. (Guangzhou, China). Aspartate aminotransferase (AST) kit (Cat# C010-2-1), alanine aminotransferase (ALT) kit (Cat# C009-2-1), superoxide dismutase (SOD) kit (Cat# A001-3-2), and malondialdehyde (MDA) kit (Cat# A003-1-2) were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). ELISA kits (Rat tumor necrosis factor-alpha (TNFα), Cat# ERA56RB; Rat interleukin 6 (IL-6), Cat# ERA31RBX5) were purchased from Thermo Fisher, USA.

Li J., Song D., Zhang B., Guo J., Li W., Zhang X, & Zhao Q. (2021). Hepatoprotective Effects of Heracleum candicans Against Carbon Tetrachloride-Induced Acute Liver Injury in Rats. Dose-Response, 19(3), 15593258211029510.

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Hepatic Damage Analysis in Mice

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At the end of in vivo experiment, serum samples of each mouse were collected and used to test hepatic damage by aspartate aminotransferase (AST) and alanine aminotransferase (ALT) kit (Nanjing Jiancheng Biological Engineering Research Institute, C010-2-1, C009-2-1, China) according to the manufactures’ instructions. The heart, liver, spleen, lung and kidney samples of each group were collected and fixed by 4% paraformaldehyde solution, and sent to the Wuhan Service Biotechnology company in China for H&E staining assay.

Chen C., Sui X., Ning H., Sun Y., Du J., Chen X., Zhou X., Chen G., Shen W., Pang L., Zhou X., Shi R., Li W., Wang H., Zhao W., Zhai W., Qi Y., Wu Y, & Gao Y. (2022). Identification of natural product 3, 5-diiodotyrosine as APOBEC3B inhibitor to prevent somatic mutation accumulation and cancer progression. Journal for Immunotherapy of Cancer, 10(11), e005503.

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DNA Protective Effects in Liver Injury

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Sodium deoxyribonucleic acid salt (derived from salmon sperm) was purchased from Sigma. The mass concentration of DNA in the sodium deoxyribonucleic acid salt of 0.02 mg/mL was determined by NanoDrop to be 1.25 mg DNA/mg sodium deoxyribonucleic acid sodium salt. Sodium carboxymethyl cellulose (0.5%) was used to prepare suspensions containing DNA with final concentrations of 10 mg/kg (low dose), 50 mg/kg (medium dose), and 300 mg/kg (high dose), respectively. Olive oil was purchased from Hangzhou Guanlai Trading Co. Ltd.; CCl₄ was purchased from Sinopharm Chemical Reagent Co. Ltd, diluted with olive oil. Aspartate aminotransferase (AST) kit, alanine aminotransferase (ALT) kit, superoxide dismutase (SOD) kit, malondialdehyde (MDA) kit, glutathione peroxidase (GSH‐Px) kit, and glutathione (GSH) kit were all purchased from Nanjing Jiancheng Biological Engineering Co. Ltd, while ELISA kits TNF‐α and IL‐6 were purchased from Thermo Fisher Scientific, USA. TUNEL (terminal deoxynucleotidyl transferase (TdT)‐mediated dUTP nick end labeling) apoptosis kit and bicinchoninic acid (BCA) protein concentration determination kit were purchased from Beijing Solarbio Science & Technology Co. Ltd. β‐Actin antibody was purchased from Affinity and other antibodies were purchased from ProteinTech Group.

Huang X., Chu X., Tian Y., Xue Y., Zhang L., Li J., Hou H., Dong P, & Wang J. (2022). Preventive effect of salmon sperm DNA on acute carbon tetrachloride‐induced liver injury in mice through Nrf2/ARE and mitochondrial apoptosis pathway. Food Science & Nutrition, 11(2), 733-742.

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Antioxidant Biomarkers in ICR Mice

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The animal experiment was approved by the Experimental Animal Care Ethics Committee of Northwest A&F University. A total of 320 ICR mice (male, 9 weeks old) with an average weight of 33 ± 2 g, SPF grade, were purchased from Vital River Laboratory Animal Technology Co., Ltd. (Beijing, China) (Approval Number: SCXK (Beijing) 2012-0001). Before the experiment, all experimental animals were bred adaptively in the animal room for one week. The conditions in the animal room were controlled as follows: the humidity was set to 55% ± 5% and the temperature was set to 25 ± 2 ℃, a 12-h circadian rhythm was ensured, and the experimental animals could drink water and eat freely. After all the mice had adapted to the environment, the experiment was started.
The aspartate aminotransferase (AST) kit, the alanine aminotransferase (ALT) kit, the total bilirubin (TBIL) kit, the alkaline phosphatase (ALP) kit, the total triglyceride (TC) kit, the total cholesterol (TG) kit, the total antioxidant capacity (T-AOC) kit, the catalase (CAT) kit, the glutathione (GSH) kit, the superoxide dismutase (SOD) kit, and the malonaldehyde (MDA) kit were purchased from Nanjing Jiancheng Bioengineering Institute (China).

Sun X., Wang J., Ge Q., Li C., Ma T., Fang Y, & Zhan J. (2022). Interactive Effects of Copper and Functional Substances in Wine on Alcoholic Hepatic Injury in Mice. Foods, 11(16), 2383.

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Palmitic Acid Induced Hepatic Injury

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Human L02 hepatic cell lines (Wanleibio, Shenyang, China), RPMI-1640 medium and fetal bovine serum (Gibco, USA), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT)(Sigma, USA), dimethyl sulfoxide (DMSO) (Sigma); Oil Red O (Sigma), fluo-3/AM kit (Beyotime Biotech, Shanghai, China), palmitic acid (Sigma), alanine aminotransferase (ALT) kit (Nanjing Jiancheng Bioengineering Institute, China), and Salidroside (purity >98%, Aladdin Biotech, Shanghai, China). The antibodies included anti-TRPM2 (Proteintech, Wuhan, China), anti-phospho-CaMKII (Proteintech), anti-LC3B (Wanleibio), anti-p62 (Wanleibio) and goat anti-rabbit secondary antibody (Wanleibio); Trizol reagent (Invitrogen, CA, USA); Reverse Transcription Kit (BioTeke, Beijing, China); Sybr Green (Solarbio, Beijing, China).

Feng Q., Liu C., Gao W., Geng X.L, & Dai N. (2019). Salidroside-Mitigated Inflammatory Injury of Hepatocytes with Non-Alcoholic Fatty Liver Disease via Inhibition TRPM2 Ion Channel Activation. Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy, 12, 2755-2763.

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