Leica emcpc cryo chamber

The samples were prepared in the same way as described in the Laurdan-stained liposomes preparation without adding Laurdan.
A 4-μL aliquot of sample was deposited on an EM grid coated with a perforated carbon film. After draining the excess liquid with a filter paper, grids were plunge-frozen into liquid ethane cooled by liquid nitrogen using a Leica EMCPC cryo-chamber (Leica Microsystems, Wetzlar, Germany). For cryo-TEM observation, grids were mounted onto a Gatan 626 cryoholder and transferred to a Tecnai F20 microscope (Thermo Fisher Scientific) operated at 200 kV. Images were recorded with an Eagle 2k CCD camera (FEI, Hillsboro, OR, USA).

A 4 μL aliquot was placed onto an EM grid coated with a perforated carbon film. Excess liquid was removed with filter paper, and grids were plunge-frozen into liquid ethane cooled by liquid nitrogen using a Leica EMCPC cryo-chamber (Leica Microsystems, Milton Keynes, UK). For Cryo-EM observation, grids were mounted onto a Gatan 626 cryoholder (Gatan Inc., Pleasanton, CA, USA) and transferred to a Tecnai F20 microscope (ThermoFisher, Waltham, MA, USA) operated at 200 kV. Images were captured with an Eagle 2k CCD camera (FEI, Hillsboro, OR, USA).