Human bcl 2 platinum elisa kit

Bcl-2 cellular protein concentration was quantified using the Human Bcl-2 Platinum ELISA Kit (Affymetrix eBioscience, Vienna, Austria) based on the manufacturer’s instruction. The kit is able to detect the human Bcl-2 by solid-phase sandwich ELISA which is designed to measure the amount of target bound between a matched antibody pair. The cells were seeded in T75 cm² flasks (Nunc) and treated with different concentration of MS13 (EC₅₀ and 2x EC₅₀) at 24, 48 and 72 h. Each experiment included a set of control cells (media with DMSO). The concentration of protein lysate was also determined by using Pierce BCA Protein Assay Kit (Thermo Scientific, United States). The Bcl-2 concentrations of the samples were obtained by comparing the absorbance obtained against the standards at 450 nm by using a microplate spectrophotometer (BioTek™ EON™ Microplate Spectrophotometers, Fisher Scientific, United States). Data was presented in the fold-change of absorbance from treated cells against absorbance from untreated cells (control) based on the equation below: $\text{Fold}-\text{change}=\frac{\text{Absorbance}\text{reading}\left(450\text{nm}\right)\text{of}\text{treated}\text{cells}}{\text{Absorbance}\text{reading}\left(450\text{nm}\right)\text{of}\text{untreated}\text{cells}}$

Bcl-2 cellular protein concentration was quantified using the Human Bcl-2 Platinum ELISA kit (Affymetrix eBioscience, Vienna, Austria) following the manufacturer’s instruction. Briefly, SW480 and SW620 cells were cultured and treated for 24, 48, and 72 h. The color intensity was measured at 450 nm by using a microplate spectrophotometer (BioTek™ EON™ Microplate Spectrophotometers, Fisher Scientific, USA). Bcl-2 concentrations from the samples were obtained by comparing the absorbance obtained against the standards. Data was presented in fold-change of absorbance from treated cells against absorbance from untreated cells: $\begin{array}{cc}\hfill Fold-change& =\left(Abs. reading \left(400nm\right) of treated cells\right)\hfill \\ & ⁄\left(Abs. reading \left(400nm\right) of untreated cells\right)\hfill \end{array}$