Franz Diffusion Cell Apparatus (Perme-Gear, Hellertown, PA, USA) was used for investigation of in vitro drug release study. Artificial membrane (Tuffryn membrane) (diameter 2.5 mm and pore size 0.45 µm) was employed on the diffusion cells and 2.5 cm2 area of formulated transdermal patch was fixed over artificial membrane. The receptor compartments were filled with phosphate buffer (pH 5.5). The receptor compartments were maintained at 32 ± 0.5 °C temperature with continues stirring of magnetic stirrer beads were fixed over 100 rpm. Then, 2 mL aliquots were obtained at specified time intervals, i.e., 0.5, 1, 1.5, 2, 4, 8, 12, 16, and 24 h, respectively. The sink condition of receptor compartments was carried out with the addition of fresh buffer (pH 5.5). The collected samples were evaluated using UV visible spectrophotometer at 303 nm wave length [18 (link)].
Franz diffusion cell apparatus
Manufactured by PermeGear
Sourced in United States
The Franz Diffusion Cell Apparatus is a laboratory equipment used to measure the diffusion rate of materials across a membrane or barrier. It consists of two chambers separated by a sample holder that can accommodate a membrane or barrier material. The apparatus allows for the controlled introduction and monitoring of substances on both sides of the membrane, enabling the evaluation of diffusion properties.
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Lab products found in correlation
6 protocols using franz diffusion cell apparatus
1
In Vitro Drug Release Profiling of Transdermal Patches
2
In Vitro Release of Curcumin Nanoparticles and Patches
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The in vitro release of nanoparticles and patches was performed using Franz Diffusion Cell Apparatus (Perme-Gear, Hellertown, PA, USA) and synthetic membrane (Tuffryn membrane; diameter 2.5 mm and pore size 0.45 µm). The membrane was fixed between donor and receptor compartment. The receptor compartment was filled with phosphate buffer (pH 5.5) and 1.5% polysorbate 80 in-simulation to skin pH. The temperature of the receptor compartment was maintained at 32 ± 0.5 °C and stirred at 100 rpm. The donor compartment was charged with Cur-Sol (curcumin solution), Cur-Cs-Np (curcumin chitosan nanoparticles), Cur-P (Curcumin patch), and Cur-Cs-Np-P (curcumin chitosan nanoparticle patch), each containing 5 mg of drug. Then, 2 mL sample was collected at specified time intervals, i.e., 0.5, 1, 1.5, 2, 4, 8, 12, 16, and 24 h, respectively, and replaced with equal volume of fresh buffer (pH 5.5) to maintain the sink condition. The collected samples were analyzed using UV visible spectrophotometer at 425 nm wave length [31 (link)].
3
Methotrexate Transdermal Patch In Vitro Release
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An in vitro drug release study for the methotrexate-loaded transdermal patches was completed using a Franz Diffusion Cell Apparatus (PermeGear, 1815 Leithsville Rd, Hellertown, PA, 18055, USA, +1 484-851-3688). For the in vitro drug release study of the methotrexate-loaded transdermal patches, a Tuffryn membrane was used. The Tuffryn membrane was placed on the diffusion cells’ and a 1cm2 area of transdermal patch was fixed over the Tuffryn membrane. Receptor compartments were filled with phosphate buffer (pH 5.5). The temperature of the receptor compartments was maintained at 32 ± 0.5 °C with constant stirring at 100 rpm using the beads of a magnetic stirrer. At specific time intervals of 0.5, 1, 1.5, 2, 4, 8, 12, 16, and 24 h, 2 mL samples were collected in test tubes and replaced with fresh phosphate buffer (pH 5.5) at equal volume for maintaining sink conditions. The collected samples were analyzed spectrophotometrically at 303 nm wavelength [26 (link)].
4
In Vitro Release Study of Nanoemulsion Gel
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The in vitro release behavior of the prepared nanoemulsion gel formulations was evaluated using a Franz diffusion cell apparatus (Perme-Gear, Hellertown, PA, USA). The partitioning of the donor and receptor compartment was carried out using a Tuffryn membrane (artificial membrane) with a pore size 0.45 µm. Fresh phosphate buffer (pH 5.5) was used in the receptor compartment as a simulation of skin pH. The temperature was maintained at 32 ± 2 °C as a simulation to skin temperature. From the receptor compartment, 1 mL sample was collected, and 1 mL fresh buffer solution (pH 5.5) was added to the receptor compartment in order to maintain a sink condition. The collected samples were analyzed at λmax 303 using a UV visible spectrophotometer. Triplicate results were observed as mean ± SD.
The data obtained from the in vitro release study were fitted in a power law equation:
where Mt/M∞ represents the release of the drug, k represents the power constant, and n represents the exponent of diffusion. The n value showed drug transport behavior [31 (link)].
The data obtained from the in vitro release study were fitted in a power law equation:
where Mt/M∞ represents the release of the drug, k represents the power constant, and n represents the exponent of diffusion. The n value showed drug transport behavior [31 (link)].
5
In Vitro Drug Release Study of Microspheres
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In vitro DSP release study was performed using a Franz diffusion cell apparatus (25 mm: 20 mL volume, PermeGear, Hellertown, PA, USA) in which the receiver compartment was filled up with SNF and thermostated at 34 °C. In preliminary experiments, DSP was proven to be stable in SNF, at both 25 °C and 34 °C for 24 h. Sink conditions were provided during the whole experiment. The receiving medium was magnetically stirred during the whole experiment. The amount of (microspheres) powder sample containing 1.5 mg of DSP was weighed on the polyamide membrane (0.45 μm pore size) inserted between the donor and the receiver compartment. At scheduled time intervals, the samples (0.5 mL) were withdrawn from the receiver compartment and replaced with fresh medium in the total duration of 3 h. DSP content in the taken samples was determined by HPLC method as described in the Section 2.20 . Each experiment was performed in triplicate.
6
Drug Release from FNS-NSs via Franz Diffusion
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Drug release from FNS-NSs was assessed through Franz Diffusion Cell apparatus (PermeGear, USA) using an artificial membrane, ie polytetrafluoroethylene (PTFE) (0.45 µm pore size; Sartorius AG, Goettingen, Germany) at 32 °C. A specific quantity of each formulation was introduced in the donor compartment. Similarly, the receptor chamber was filled with a specific amount of acetate buffer as a drug release medium. Additionally, throughout the test, the sink condition was maintained along with magnetic stirring at 150 rpm. To evaluate the drug release behavior, aliquots (500 µL) were withdrawn at specific time intervals (0, 0.5, 1, 2, 4, 8, 12, 16 and 24 h) and then compensated with 0.5 mL of fresh buffer medium kept at 32 °C. The samples were analyzed to check the FNS released pattern using the UV/visible spectrophotometer at 210 nm. The analysis was performed in triplicate to minimize any error and the results were averaged.
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