Static contact angle measurements were performed on N2/CyA-ASN and Fx/N2CyA-ASNg formulations before and after the gelling process (Fx/N2CyA-ASNg/ATF) using an optical contact angle-measuring instrument (OCA 15, DataPhysics Instrument GmbH, Filderstadt, Germany). The system consisted of a high-resolution CCD video camera and a six-fold power zoom lens with integrated fine focusing; the images were recorded and analyzed by SCA 20 software. The sessile drop method was applied as follows: a volume of 5.0 μL of each formulation was dropped onto a glass slide at a rate of 50 μL min−1. When the spreading droplet achieved an equilibrium state, the image was taken and analyzed by the Laplace–Young fit method. Six measurements were made for each formulation [37 (link)].
Oca 15
Manufactured by Dataphysics
Sourced in Germany, United States
The OCA 15 is a high-precision contact angle measurement instrument designed for characterizing the wetting properties of solid surfaces. It utilizes an optical system to capture and analyze images of liquid droplets on a sample surface, providing accurate measurements of static, dynamic, and advancing/receding contact angles.
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Lab products found in correlation
Sca20, by Dataphysics (6 mentions)
Leo 1530, by Zeiss (2 mentions)
Nanodrop one, by Thermo Fisher Scientific (2 mentions)
Lsm 700, by Zeiss (2 mentions)
Sigma 300, by Zeiss (2 mentions)
E600 upright fluorescence microscope, by Nikon (1 mentions)
Jsm 5610, by JEOL (1 mentions)
Spectrum 100 ftir spectrometer, by PerkinElmer (1 mentions)
Dimension 3100 afm, by Veeco (1 mentions)
Multimode spm, by Veeco (1 mentions)
Nicolet6700 contiuμm, by Thermo Fisher Scientific (1 mentions)
Labram hr evolution, by Horiba (1 mentions)
Sp 300 electrochemical workstation, by Bio-Logic (1 mentions)
Su8000, by Hitachi (1 mentions)
Icap rq, by Thermo Fisher Scientific (1 mentions)
Vk x1000, by Keyence (1 mentions)
X max50, by Oxford Instruments (1 mentions)
Vector 33, by Bruker (1 mentions)
Jsm 7001f, by JEOL (1 mentions)
Monoatomic cluster ion gun, by Thermo Fisher Scientific (1 mentions)
59 protocols using oca 15
1
Static Contact Angle Measurements of Novel Formulations
2
Surface Wettability Characterization
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The water contact angle was determined through goniometry using a static water drop method. Contact angles were measured with distilled water using the contact angle measuring system (OCA15; Dataphysics, Germany). A drop of water with a volume of 4 ± 0.2 μL was deposited on the surface.
3
Contact Angle Measurements of Hydrophobic Surfaces
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Contact angle measurements were performed using the sessile drop method in a contact angle meter OCA15+ (DataPhysics Instruments) with highperformance image processing system. Drops of distilled water, with a volume of 2 µL and 10 µL were added by a motor driven syringe, at room temperature.
Five measurements were taken in different regions of 3 cut discs for each composition and an averaged value was calculated.
According to Wenzel model [28, 29] roughness increases the surface area of a solid, enhancing its hydrophobicity. Moreover, air can remain trapped below the drop, leading to a super hydrophobic behavior, in which the drop sits partially on air creating a non-filling regime where the liquid does not penetrate into surface grooves (Cassie-Baxter model) [28] . Depending on the applied model (filling or non-filling regime) Young contact angle (θY) values can be calculated using the Wenzel equation [29] described by
where Rw symbolizes the surface area ratio, also referred to as Wenzel factor and calculated by
and θm is the measured (also called apparent) contact angle, or using the Cassie-Baxter equation [29] described by
where φS is the fraction of solid in contact with the liquid.
Five measurements were taken in different regions of 3 cut discs for each composition and an averaged value was calculated.
According to Wenzel model [28, 29] roughness increases the surface area of a solid, enhancing its hydrophobicity. Moreover, air can remain trapped below the drop, leading to a super hydrophobic behavior, in which the drop sits partially on air creating a non-filling regime where the liquid does not penetrate into surface grooves (Cassie-Baxter model) [28] . Depending on the applied model (filling or non-filling regime) Young contact angle (θY) values can be calculated using the Wenzel equation [29] described by
where Rw symbolizes the surface area ratio, also referred to as Wenzel factor and calculated by
and θm is the measured (also called apparent) contact angle, or using the Cassie-Baxter equation [29] described by
where φS is the fraction of solid in contact with the liquid.
4
Water Contact Angle Measurement
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Contact angle measuring system from Data Physics, model OCA 15, equipped with a video CCD-camera and SCA 20 software was used with sessile drop method, as described at53 (link). Drops of type I water (4 µL) were deposited onto the samples surfaces and images were taken every 2 s over 300 s. Droplet profiles were fitted using Young-Laplace formula, and the WCA of each sample was calculated by extrapolating the time dependent curve to zero. Results are the average of two measurements on three independent samples.
5
Water Contact Angle Measurement
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WCA was characterized with a contact angle goniometer (OCA15, Dataphysics, Germany) at 25 °C using distilled water as a reference liquid. A total of 1.00 μL of reference liquid was pumped onto the surface through a stainless steel needle at a rate of 1.0 μL/s. The results are mean values calculated from five independent measurements on different points of the films.
6
Water Contact Angle Measurement
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Contact angles on modified epoxy glass slides were recorded using a Dataphysics OCA 15 plus Contact Angle measuring device equipped with SCA 20 software (Filderstadt, Germany) based on sessile drop method. A total of five samples were tested for each configuration using 8 µL distilled de‐ionized (DDI) water droplet at the ejection speed of 1 µL s−1.
7
Particle Characterization: A Comprehensive Approach
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Pristine and modified particles were characterized by various techniques. The particle size was determined via image analysis of transmission electron microscopy (TEM) micrographs. The density of a single particle was determined by measuring the density of particle suspensions at different particle concentrations using a densitometer (DM40, Mettler Toledo). The hydrophobicity was quantified the contact angle measurements of a water droplet on a spin coated particle layer (OCA 15, DataPhysics). Atomic force microscopy (AFM) studies were carried out in order to ensure similar roughness of the particle layer. The charge of the particles was determined by ζ -potential measurements. Further information of the methods and the instrumentation are given in the supporting information.
8
Characterization of OTFT Electrical Properties
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The electrical
characteristics of
OTFTs were measured using a Keithley 4200 semiconductor analyzer,
and the capacitance of soluble polymer films was characterized on
an Agilent 4294A. Polarized optical micrographs of C8-BTBT-based films
were obtained using a CPV-900 reflectance polarizing microscope. The
contact angle of the surface was measured with a video-based automatic
contact angle measuring instrument (OCA15, Data Physics). X-ray diffraction
(XRD) patterns (2q scans) were obtained using a Bruker Advanced D8
X-ray diffractometer using Cu Kα (λ = 0.154 nm) radiation.
characteristics of
OTFTs were measured using a Keithley 4200 semiconductor analyzer,
and the capacitance of soluble polymer films was characterized on
an Agilent 4294A. Polarized optical micrographs of C8-BTBT-based films
were obtained using a CPV-900 reflectance polarizing microscope. The
contact angle of the surface was measured with a video-based automatic
contact angle measuring instrument (OCA15, Data Physics). X-ray diffraction
(XRD) patterns (2q scans) were obtained using a Bruker Advanced D8
X-ray diffractometer using Cu Kα (λ = 0.154 nm) radiation.
9
Collagen IV Functionalization of PLA Films
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The physico-chemical characterization of the PLA films during the different steps of the functionalization process has already been reported elsewhere [39] (link). Contact angle (CA) measurements were carried out with an optical contact angle device (OCA15, Dataphysics, Germany) before and after col IV functionalization of the PLA films.
Immunofluorescence was used to determine the presence of col IV on the surface of the PLA films. In brief, the samples were blocked for 20 min at room temperature with PBS-Glycine-bovine serum albumin.
After washing with PBS-Glycine (2 × 5 min), the samples were incubated with the primary antibody (Table 2) for 45 min at 37 °C and washed with PBS-Glycine (2 × 5 min). The incubation of the secondary antibody (Table 2) was performed in the dark over 45 min at 37 °C. The samples were visualized with a Nikon E600 upright fluorescence microscope (Tokyo, Japan). Different controls were performed by incubation of either only primary or secondary antibodies.
For col IV quantification, a Micro BCA™ Protein Assay Kit was used, following the manufacturer's protocol. The experiment was conducted in triplicate with six replicas per experiment.
Immunofluorescence was used to determine the presence of col IV on the surface of the PLA films. In brief, the samples were blocked for 20 min at room temperature with PBS-Glycine-bovine serum albumin.
After washing with PBS-Glycine (2 × 5 min), the samples were incubated with the primary antibody (Table 2) for 45 min at 37 °C and washed with PBS-Glycine (2 × 5 min). The incubation of the secondary antibody (Table 2) was performed in the dark over 45 min at 37 °C. The samples were visualized with a Nikon E600 upright fluorescence microscope (Tokyo, Japan). Different controls were performed by incubation of either only primary or secondary antibodies.
For col IV quantification, a Micro BCA™ Protein Assay Kit was used, following the manufacturer's protocol. The experiment was conducted in triplicate with six replicas per experiment.
10
Scaffold Wettability Characterization
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The wettability of scaffolds was determined by sessile drop technique using an optical contact angle device (OCA15, Dataphysics, Germany). Sessile milli-Q water drop was deposited onto sample surface with the syringe, and the drop contour was fitted by the Young-Laplace method. At least 3 different static contact angle measurements on each sample were performed.
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