The L4–L6 spinal cord segments were collected and made into single cell suspension. The mononuclear cells were isolated by density gradient centrifugation using Percoll and stained with anti-CD11b. The cells were then stained with the following fluorochrome-labeled antibodies: anti-CD68-PE (Invitrogen) and anti-CD206-Alexa Fluor 647 (Abcam). The cells were then fixed and analyzed with an Accuri C6 flow cytometer (Becton Dickinson, Franklin Lakes, NJ, USA).
Anti cd68 pe
Manufactured by Thermo Fisher Scientific
Anti-CD68-PE is a fluorochrome-conjugated antibody that binds to the CD68 antigen, which is expressed on the surface of monocytes, macrophages, and certain dendritic cells. It is used in flow cytometry applications to identify and quantify these cell populations.
Automatically generated - may contain errors
Lab products found in correlation
Accuri c6 flow cytometer, by BD (1 mentions)
Anti cd11b pe cy7, by BioLegend (1 mentions)
Anti cd45 apc cy7, by BioLegend (1 mentions)
Anti cd11c apc, by BioLegend (1 mentions)
Anti cd16 cd32 antibody, by BioLegend (1 mentions)
Anti cd16 percp cy5.5, by BioLegend (1 mentions)
Anti cd15 fitc, by BioLegend (1 mentions)
2 protocols using anti cd68 pe
1
Isolation and Characterization of Spinal Cord Myeloid Cells
2
Sputum Immune Cell Profiling by Flow Cytometry
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Sputum cells were blocked with anti-CD16/CD32 antibodies (BioLegend, USA) for 10 min and then stained with Live_Dead (Zomnie Aqua, BioLegend) for 20 min at 4 °C. Human induced sputum cells were then stained with the following antibodies: anti-CD45 (APC/Cy7; BioLegend), anti-CD11c (APC; BioLegend), anti-CD68 (PE; Invitrogen, CN), anti-CD86 (PE/Dazzle594; BioLegend), anti-CD16 (Percp/Cy5.5; BioLegend), anti-CD11b (PE/Cy7; BioLegend), and anti-CD15 (FITC; BioLegend). Flow cytometry was performed with a Cytek Dxp Athena flow cytometer, and the data were analyzed by using FlowJo (version 10) software.
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