Gold 3 chloride trihydrate acs reagent

Manufactured by Merck Group

Sourced in United States

Gold (III) chloride trihydrate (ACS reagent) is a chemical compound with the formula AuCl3⋅3H2O. It is a crystalline solid that is used as a precursor in various chemical reactions and applications. The product meets the standards set by the American Chemical Society (ACS) for reagent-grade chemicals.

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Gold(III) chloride trihydrate (296 citations) ACS reagent (167 citations) Gold chloride trihydrate (57 citations) Gold(III) chloride (17 citations) Gold chloride (14 citations)

3 protocols using gold 3 chloride trihydrate acs reagent

Rapid Immunoassay Diagnostic Kit

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Gold (III) chloride trihydrate (ACS reagent), hydroquinone, streptococcal protein G, sodium caseinate, anti-human IgM (µ chain specific) antibody produced in goat, and bovine serum albumin (BSA)were purchased from Sigma–Aldrich (St. Louis, MO, USA). Tween20 and other chemicals were of analytical grade and were obtained from VWR International (Milan, Italy). HIV-antigens gp36 and gp41 were purchased from Arista Biologicals Inc. (Allentown, PA, USA). Casein-biotin for conjugation to GNPs was obtained from In3diagnostics (Torino, Italy). Nitrocellulose membranes with cellulose adsorbent pad (CNPC-SS12-L3-P25) and sample pads (FR-1) were purchased from MDI membrane technologies (Ambala, India), while conjugate pads (GF) were obtained from Merck Millipore (Billerica, MA, USA).

Cavalera S., Di Nardo F., Forte L., Marinoni F., Chiarello M., Baggiani C, & Anfossi L. (2020). Switching from Multiplex to Multimodal Colorimetric Lateral Flow Immunosensor. Sensors (Basel, Switzerland), 20(22), 6609.

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SARS-CoV-2 Antibody Detection ELISA

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Gold (III) chloride trihydrate (ACS reagent), staphylococcal protein A (SpA), casein sodium salt from milk, avidin, sucrose, polyethylene glycol 10,000 (PEG), bovine serum albumin (BSA) were obtained from Sigma–Aldrich (St. Louis, MO, USA). Tween20 and other chemicals were purchased from VWR International (Milan, Italy). Nitro-cellulose membranes with cellulose adsorbent pad and blood separator sample pads were purchased by MDI membrane technologies (Ambala, India) and glass fibre conjugate pads were obtained from Merck Millipore (Billerica, MA, USA). The ELISA kit was an indirect ELISA for the detection of anti SARS-CoV2 antibodies (ERADIKIT™ COVID19-IgG from In3diagnostic srl, Turin, Italy) [24 ]. The commercial ELISA kit was registered as IVD-CE according to European Directive 98/79/CE for the detection of IgG in serum samples. The performances declared by the manufacturer are: sensitivity of 96% if the ELISA test is performed on samples collected after 20 days after the first positive swab; analytical and diagnostic specificity of 100%; repeatability and reproducibility: Coefficient of Variation <5%. Statistical calculations were carried out with SigmaPlot 11.0 software.

Cavalera S., Colitti B., Rosati S., Ferrara G., Bertolotti L., Nogarol C., Guiotto C., Cagnazzo C., Denina M., Fagioli F., Di Nardo F., Chiarello M., Baggiani C, & Anfossi L. (2020). A multi-target lateral flow immunoassay enabling the specific and sensitive detection of total antibodies to SARS COV-2. Talanta, 223, 121737.

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Synthesis and Characterization of Gold Nanoparticles

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Tris(2,3-epoxypropyl)isocyanurate (TEPIC), melamine (99%), tetraethylene glycol dimethyl ether (TEGDME, 99%), gold(iii) chloride trihydrate ACS reagent(≥49.0%, HAuCl₄·3H₂O), 4-nitrophenol (99%, 4-Nph) and sodium borohydride (≥98.0%, NaBH₄) were purchased from Sigma Aldrich. Acetone (ACS, ISO, Reag. PhEur) was obtained from Merck Chemical Company. All chemicals were used as received without any further purification. The water used in all experiments was deionized water with a resistivity of 18 MW cm⁻¹, which was prepared by an ultrapure water system (Aqua Solutions).

Dursun S., Yavuz E, & Çetinkaya Z. (2019). In situ reduction of chloroauric acid (HAuCl4) for generation of catalytic Au nanoparticle embedded triazine based covalent organic polymer networks. RSC Advances, 9(66), 38538-38546.

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