The BEAS-2B cell line was purchased from the Chinese Academy of Sciences Cell Bank (CASCB, China), and cultured in BEGM media (Lonza, CC-3170). Lung cancer cell lines, including H358, H1650, A549, and H1299, were purchased from CASCB with STR documents, and were cultured in RPMI-1640 medium (Corning) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin.
H1650
Manufactured by Corning
Sourced in China
The H1650 is a laboratory equipment product manufactured by Corning. It is designed to perform specific functions in a laboratory setting. The product's core function is to provide a controlled environment for various applications, but a detailed description of its intended use is not available.
Automatically generated - may contain errors
Lab products found in correlation
Rpmi 1640 medium, by Corning (7 mentions)
H1975, by Corning (4 mentions)
H1975, by Cobioer Biosciences (3 mentions)
H1650, by Cobioer Biosciences (3 mentions)
H1299, by Corning (3 mentions)
Dmem medium, by Corning (3 mentions)
Cc 3170, by Lonza (2 mentions)
Beas 2b cell line, by National Collection of Authenticated Cell Cultures (2 mentions)
Hcc827, by Corning (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
H1299, by American Type Culture Collection (2 mentions)
Bronchial epithelial cell growth medium, by Lonza (1 mentions)
H1299, by Cobioer Biosciences (1 mentions)
Hek293t, by Corning (1 mentions)
Bronchial epithelial growth medium (begm), by Lonza (1 mentions)
Hek293t, by American Type Culture Collection (1 mentions)
Hcc827, by Cobioer Biosciences (1 mentions)
Lhc medium, by Thermo Fisher Scientific (1 mentions)
Beas 2b, by Thermo Fisher Scientific (1 mentions)
H1650, by American Type Culture Collection (1 mentions)
7 protocols using h1650
1
Cell Culture Protocols for Lung Cancer
2
Cultured Cell Line Characterization
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The BEAS-2B cell line was purchased from the Chinese Academy of Sciences Cell Bank (CASCB, China) and cultured in Bronchial Epithelial Cell Growth Medium (Lonza, CC-3170). The lung cancer cell lines, including HCC827, H1650, A549, and H1975, were purchased from the CASCB (China) with STR documents and were cultured in RPMI-1640 medium (Corning) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin.
3
Genetic Manipulation of PAQR4 in Lung Cells
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Independent shRNAs targeting PAQR4 were constructed using a pLKO.1 vector. The two independent PAQR4 targeting sequences are: shRNA#1, 5'-GCAGGCTCCGTGCTCTATCAC-3'; shRNA#2, 5'-CGTCTTGCTCTGAGAGTTCAA-3'. The pNFE2L2 (NRF2)-ENTER (Gene ID: NM_006164) and pKEAP1-ENTER (Gene ID: NM_203500) plasmids were purchased from Vigene Biosciences Inc., and were sub-cloned into pCDNA3.1. Nrf2 was N-terminal tagged with 6×Myc, and Keap1 was N-terminal tagged with 3×HA. The full length cDNA of PAQR4 (Gene ID: NM_152341.5) was synthesized by Shanghai Generay Biotech, and sub-cloned into pCDH-MSCV-E2F-eGFP lentiviral vector or pCDNA3.1 vector with a 3×Flag at the C-terminus. The lentiviruses were generated according to the manufacturer's protocol, stable cell lines were generated by lenti-viral infection. The BEAS-2B cell line was a gift from Dr. Hongbin Ji at SIBS, CAS, China. HEK-293T was obtained from ATCC. A549, H1299, H1975, H1650 were purchased from Cobioer, China with STR document, BEAS-2B, A549, H1650, H1975, H358, GLC-82 and SPC-A1 cells were cultured in RPMI1640 medium (Corning) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin. H1299 and HEK-293T cells were cultured in DMEM medium (Corning).
4
Cell Culture Protocols for Lung Cancer
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The BEAS-2B cell line was purchased from cell bank of Kunming Institute of Zoology, and cultured in BEGM media (Lonza, CC-3170). Lung cancer cell lines, including A549, H1650 and H1975 were purchased from Cobioer, China with STR document, A549, H1650 and H1975 cells were all cultured in RPMI1640 medium (Corning) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin.
5
Cell Culture Protocols for Lung Cancer Research
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The human bronchial epithelial (BEAS2B) cell line and LUAD cell lines were purchased from the cell bank of Kunming Institute of Zoology and cultured in bronchial epithelial cell growth media (BEGM) (Lonza, Shanghai, CC-3170). HEK-293T was obtained from the American Type Culture Collection (ATCC). Lung cancer cell lines, including H1650, HCC827, and H1975 were purchased from Cobioer (Shanghai, China) with STR document; H1650, HCC827, and H1975 cells were all cultured in Roswell Park Memorial Institute (RPMI) 1640 medium (Corning, Shanghai) supplemented with 10% fetal bovine serum (Cat. No. 10099141C, Gibco, New York, USA) and 1% penicillin/streptomycin.
6
Cell Line Characterization and Validation
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The human bronchial epithelial cell line 16HBE and the human NSCLC cell lines A549, H1650, PC‐9, 95‐D, and SPCA‐1 were all obtained from the Cell Bank, China Academy of Sciences (Shanghai, China) in 2015. The H1299 and H23 cell lines were obtained from the American Type Culture Collection (ATCC, Manassas, USA) in 2014. 16HBE, A549, PC‐9, 95‐D, and SPCA‐1 cells were cultured in DMEM medium (Corning Cellgro, Manassas, USA) supplemented with 10% (v/v) fetal bovine serum (FBS, Gibco, Gaithersburg, USA). H1299, H23, and H1650 cells were cultured in RPMI 1640 medium (Corning Cellgro) supplemented with 10% (v/v) FBS (Gibco). Culture conditions were 37°C in a 5% CO2 atmosphere. Multiple frozen master stocks were prepared within the first month from receipt for each cell line. The H1299 cell line was authenticated using short tandem repeat analysis on March 25, 2016 by Shanghai Biowing Applied Biotechnology Co. LTD, Shanghai, China, and further verified by morphology before use with images taken. Experiments were performed on H1299 cells resuscitated from master stocks and passaged for no more than 6 months. Absence of mycoplasma contamination was routinely confirmed by PCR analyses.14
7
Cell Line Cultivation and Maintenance
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The human lung epithelial cell line BEAS-2B and the human NSCLC cell lines A549 were obtained from the Cell Bank, China Academy of Sciences (Shanghai, China). The H23, H1299 and H1650 cell lines were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). A549 cells were all cultured in DMEM medium (Corning Cellgro, Manassas, VA, USA) supplemented with 10% fetal bovine serum (FBS, Gibco, Gaithersburg, MD, USA). H23, H1299 and H1650 cells were cultured in RPMI 1640 medium (Corning Cellgro) supplemented with 10% FBS (Gibco). BEAS-2B cells were cultured in LHC medium (Gibco) supplemented with 10% FBS (Gibco). Culture conditions for all cells were at 37 °C in a humidified 5% CO2 atmosphere.
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