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5 protocols using quercetrin

1

Phytochemical Profiling of Indian Medicinal Plants

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Bark of C. arborea, young leaves of P. guajava and fruit peel of P. granatum were collected in the Belagavi district of Karnataka, India. The collected plants and their parts were identified and authenticated by a certified plant taxonomist at ICMR-NITM, Belagavi. The voucher specimens were deposited in ICMR-NITM under accession numbers 1590 (C. arborea), 1690 (P. granatum), 1589 (P. guajava). Extraction of these powdered plant parts was carried out using 70% ethanol (v/v) in water by cold maceration technique (Ćujić et al., 2016 (link)). Six herbal compounds, including three phenolic acids, Ellagic acid (EA), Chlorogenic acid (CHL), Gallic acid (GA), and three flavonoids; Rutin (RTN), Phloridzin (PHD), and Quercetrin (QTRN), selected using in silico studies were procured from Sigma Aldrich. Quantification of these six compounds in hydro-alcoholic extract was performed using High-Performance Thin-Layer Chromatography (HPTLC).
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2

Comprehensive Phytochemical Analysis Protocol

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Acetone (99.78%), methanol (99.90%), sodium carbonate (NaCO3 99.5%), sodium nitrate (NaNO3 99.0%), aluminum chloride (AlCl3 97.0%), sodium hydroxide (NaOH 97.0%), potassium ferricyanide (K3(Fe(CN)6) 99.0%), iron trichloride (FeCl3 98.0%), iron II chloride (FeCl2 98.0%) and trichloroacetic acid (TCA 98.0%) were from Biochem Chemopharma (Georgia, USA). Folin–Ciocalteu reagent was from Biochem Chemopharma (Montreal, Quebec) and 1,1-Diphenyl-2-picrylhydrazyl (DPPH 95%) was from Sigma-Aldrich (Sternheim, Germany). Gallic (99.5%), p-coumaric (98%), ferulic (99%) and caffeic acids (98%), rutin (94%), luteolin (98%), isoquercetrin (97%), quercetrin (95%) and quercetin (98%) were from Sigma-Aldrich Co (Saint-Louis, MO, USA).
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3

Dexamethasone and Flavonoids Analysis

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Dexamethasone [(Fortecortine® 8 mg – Mono ampoule) manufactured by Sigma – Tec Pharmaeutical industries – Egypt – S. A. E. under Licence of: Merck, Darmstadt, Germany]. Standard flavonoids (Luteo.6-Arabinose8-Glucose, Luteo.6-Glucose8-Arabinose, Apig.6-Arabinose8-galactose, Apig.6-rhamnose8-Glucose, Apig.6-Glucose8-rhamnose, Naringin, Rutin, Hespirdin, Rosmarinic acid, Apig.7-o-neohespiroside, Apigenin-7-Glucose, Quercetrin, Quercitin, Naringenin, Hespirtin, Kampferol, Apigenin) and saccharides (Glucuronic acid, Sucrose, Xylose, Rhaminose, Mannose, Arabinose, Manitol, Stachylose, Inulin, Fructose, Glucose) were purchased from Sigma Aldrich. Kit No. ES180S-100 purchased from Calbiotech. U.S. for E2 determination, kit No. MBS702121 purchased from My Biosource. U. S. A. for PTH determination, a commercial assay kit provided from Spinreact, Spain for Ca, P determination. Kit purchased from BioSystems Company, Spain for determination activity of alkaline phosophatase. Kit purchased from BIO Diagnostic Company, Egypt for determination activity of ACP and oxidative stress markers.
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Extraction and Characterization of Bioactive Compounds from Schisandra chinensis

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Materials and reagent S. chinensis (Lour.) Baill pieces were dried and powdered. Viscozyme L was obtained from Novozymes Co. (from Aspergillus aculeatus, 100 fungal β-glucanase units/mL, Bagsvaerd, Denmark), the Silica gel (40 μm), Octadesylsilane (50 μm), rutin, isoquercetrin, quercetrin, and quercetin were obtained from Sigma Co. (St. Louis, MO, USA). The other reagents were obtained from Duksan Co. (Seoul, Korea). HPLC/UVD (SPD-10A, Shimadzu, Co., Kyoto, Japan), Moderate pressure liquid chromatography (MPLC, Yamazen 540, Japan), HPLC/MS (Agilent Technologies, Palo Alto, CA, USA), NMR (Varian Inc., Palo Alto, CA, USA) were used in this study.
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5

Identification of Secondary Metabolites

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JAQUELINI LUBER et al.
The retention times and UV spectra of the peaks on the chromatogram were compared with those of authentic standards of different secondary metabolites classes [gallic acid, protocatechuic acid, ellagic acid, (+)-catechin, quercetrin, quercetin, myricetin and apigenin, all from Sigma Aldrich ® (St. Louis, MO, USA)] and also with literature data.
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