Har. californiae and Har. hispanica cells were grown at 37 °C in CA medium prepared with 18% SW buffered with 10 mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) (pH 7.0). Cells from early-exponential phase cells were concentrated 10–20-fold by centrifugation and resuspension in CA medium before being adsorbed to glow-discharged carbon-coated copper grids (Plano GmbH, Wetzlar, Germany) with Formvar films. The samples were washed three times in drops of sterile 2 M NaCl and subsequently stained for 15 s with sterile filtered 2% (w/v) uranyl acetate prepared in 2 M NaCl. Grids were examined using a Hitachi 7800 transmission electron microscope coupled to an EMSIS Xarosa (EMSIS GmbH, Muenster, Germany) camera or an Zeiss Leo 912 Omega with Dual Speed 2K-On-Axis charged-coupled device (CCD) camera TRS, Sharp-Eye (TRS Systems, Moorenweis, Germany).
Leo 912 omega
Manufactured by Zeiss
Sourced in Germany
The LEO 912 Omega is a transmission electron microscope (TEM) designed by Zeiss. It is a versatile instrument capable of high-resolution imaging and analysis of a wide range of materials and samples. The LEO 912 Omega provides advanced electron optics and a range of advanced features to enable detailed examination of various specimens.
Automatically generated - may contain errors
Lab products found in correlation
Mria3 feg sem, by TESCAN (3 mentions)
Xarosa, by EMSIS (1 mentions)
Hpm100, by Leica camera (1 mentions)
Em afs2, by Leica camera (1 mentions)
Cary 300 bio uv visible spectrophotometer, by Agilent Technologies (1 mentions)
Dpx 300 spectrometer, by Bruker (1 mentions)
Zetasizer nano zs90, by Malvern Panalytical (1 mentions)
D8 discover, by Bruker (1 mentions)
Labram hr800, by Horiba (1 mentions)
Carbon coated copper grids, by Quantifoil (1 mentions)
Tecnai t12, by Thermo Fisher Scientific (1 mentions)
Ceta cmos 16m camera, by Thermo Fisher Scientific (1 mentions)
Epon 812, by Serva Electrophoresis (1 mentions)
Agno3, by Merck Group (1 mentions)
Proscan hsv 2, by Oxford Instruments (1 mentions)
Quanta 250 feg sem, by Thermo Fisher Scientific (1 mentions)
Evo 40, by Zeiss (1 mentions)
Oneview cmos detector, by Ametek (1 mentions)
23 protocols using leo 912 omega
1
Cryo-EM Imaging of Haloarchaea
2
Ultrastructural Analysis of Ribbon Synapses
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The preparation of retinae from 8–10-wk-old cpx3KO and WT mice has been described previously (Mortensen et al., 2016 (link), Supplemental Experimental Procedures). Briefly, retinae were fixed in a cocktail of 4% paraformaldehyde and 2.5% glutaraldehyde in 0.1 M phosphate buffer, embedded in low-melt agarose, and sectioned into 100-µm slices using a vibratome. Retina slices were processed by high-pressure freezing (Leica HPM100) and automated freeze substitution (Leica EM AFS2) and embedded in plastic for ultramicrotomy. Electron micrographs were acquired on a transmission electron microscope (Zeiss LEO 912-Omega, 80 kV) with 25,000-fold magnification. SVs surrounding ribbons of WT and cpx3KO RB synapses were counted within 200-nm concentric shells (in nm: 0–200, 200–400, 400–600, 600–800, and 800–1,000) centered at the ribbon base. The cytoplasmic area covered by each shell was also quantified to take into account cell morphology at presynaptic sites. In Fig. 6 , image contrast was manually adjusted to ease visualization.
3
Light and Electron Microscopy of Algae
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Light microscopy images and transmission electron images of C. socialis and G. wildpretii were obtained and processed as described by [9 (link)]. A modified illumination system and cultures in plastic Petri dishes with cover glasses (0.17 mm thick) inserted into bottom and top allowed for DIC photographs of living material. An additional Leica NPL Fluotar 100/1.20 W FLUORESZENZ objective in combination with an inverted FU microscope was used. TEM digital images were obtained with the LEO 912 Omega (Carl Zeiss AG, Germany).
4
Characterization of Polymer Nanoparticles
5
Characterization of Carbon Foam Materials
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X-ray photoelectron spectroscopy (XPS, Axis Ultra DLD spectrometer with a monochromatized Al Kα X-ray source with charge neutralization) was used to analyze the chemical composition of the samples. The microsctructure of the specimens were studied by field emission scanning electron microscopy (FESEM, Carl Zeiss SMT MERLIN at 3 kV) and by transmission electron microscopy (EFTEM, LEO 912 OMEGA at 120 kV using LaB6 filament). Crystal phase assessment of the carbon foams was performed with X-ray diffraction (XRD, Bruker D8 Discover, Cu Kα radiation source) and micro-Raman spectroscopy (Horiba Jobin-Yvon LabRAM HR800, Ar+ laser source at λ = 488 nm). BET surface area measurements were carried out by N2 adsorption analysis (Tristar 3000 apparatus, Micrometrics Instrument Corp.).
6
Cryogenic TEM Imaging of Liposomes
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TEM pictures of void and EF24-loaded liposomes were acquired using a Leo 912 OMEGA instrument (Carl Zeiss, Oberkochen, Germany) operating at 120 kV. Briefly, a drop of diluted solution of liposomes was coated on carbon-coated copper grids (Quantifoil Micro Tools GmbH, Jena, Germany) and was then immediately shock-frozen in liquid ethane before loading in the microscope. For each probe three grids were prepared and all digital images were captured at a magnification of 6–12K using a Proscan HSC 2 camera [15 (link)].
7
Transmission Electron Microscopy Imaging
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All thin sections were imaged at 120 kV either on a LEO 912 OMEGA (Zeiss) equipped with a 2k × 2k VELETA Olympus CCD camera or on a Tecnai T12 transmission electron microscope equipped with a Ceta CMOS 16M camera (FEI). Tomograms were acquired with the use of serial EM (104 (link)) on a Tecnai F20 and F30 microscopes operated at 200 and 300 kV. The tomographic reconstruction was performed using the IMOD software package (105 (link)).
8
HeLa Cell Ultrastructure Examination
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HeLa cells were fixed using a double fixation protocol with osmium and tannic acid [44] (link). Samples were dehydrated in graded ethanol series, and embedded in Epon 812 (Serva). After 48 h at 60°C, ultra-thin sections (60 nm) were cut and mounted on grids. Samples were examined on a LEO 912 OMEGA (Energy Filter Transmission Electron Microscope, Zeiss) at 120 kV accelerating voltage. Digital images were obtained through a side-mounted MegaView III TEM CCD camera.
9
Characterization of Particles by VSM, TEM, and SEM
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Example 4
In this example, the analysis of particles by means of VSM measurements, TEM measurements and SEM measurements is described.
VSM Measurements
The magnetic properties of the samples obtained herein were investigated with the aid of an EV7 Vibrating Sample Magnetometer (VSM) from ADE Magnetics. The magnetization of a sample was measured in dependence on the applied field, wherein the sample was vibrated perpendicular to a uniform magnetizing field. The maximum field strength of the electromagnet used (3472-10 GMW) from Magnet Systems was 2.1 T. The sample was exposed to a vibration frequency of 75 Hz, and the hysteresis loops were recorded between −1.5·108 A·m−1 and 1.5·108 A·m−1.
TEM Measurements
The TEM measurements were carried out on a Leo 912 Omega from Zeiss. For this purpose, the samples were dripped from a particle dispersion onto a carbon-coated copper grid before the measurements, and the samples were dried at room temperature. The images were evaluated using SiViewer software and ImageJ software.
SEM Measurements
SEM micrographs were generated using a Zeiss Neon 40 ESB CrossBeam FIB-SEM scanning electron microscope. The samples were dripped from a particle dispersion onto a silica plate. The samples were coated with gold before the measurement. The particle size was evaluated after measurement using ImageJ software.
10
Fabrication and Characterization of Ag and Zn Doped ZSM-5 Zeolite
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HZSM-5 zeolite was prepared in the laboratory. Zinc nitrate, and AgNO3 were procured from Merck (Merck, Darmstad, Germany).
The powder form of ZSM-5 was modified by liquid phase ion exchange (LPIE), using Ag+ and Zn2+ cations. First, Ag+-ZSM-5 was prepared through
a 24-h ion exchange with a solution consisting of 10 g of ZSM-5 and 300 mL of silver nitrate (1M) at room temperature. Then to prepare
Ag+ and Zn2+-ZSM-5, 10 g of Ag+-ZSM-5 were added to 300 mL of zinc nitrate solution (5M). After each exchange process, the modified
zeolite suspension was filtered and washed with copious amounts of deionized water. Synthesized nanoparticles were characterized by
scanning electron microscopy MRIA3-FEG-SEM (Tescan, Brno, Czech) and transmission electron microscopy (Zeiss LEO 912 Omega).
The powder form of ZSM-5 was modified by liquid phase ion exchange (LPIE), using Ag+ and Zn2+ cations. First, Ag+-ZSM-5 was prepared through
a 24-h ion exchange with a solution consisting of 10 g of ZSM-5 and 300 mL of silver nitrate (1M) at room temperature. Then to prepare
Ag+ and Zn2+-ZSM-5, 10 g of Ag+-ZSM-5 were added to 300 mL of zinc nitrate solution (5M). After each exchange process, the modified
zeolite suspension was filtered and washed with copious amounts of deionized water. Synthesized nanoparticles were characterized by
scanning electron microscopy MRIA3-FEG-SEM (Tescan, Brno, Czech) and transmission electron microscopy (Zeiss LEO 912 Omega).
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