Conventional clinical metabolite and targeted metabolomics assays were conducted as previously described using maternal serum obtained fasting and 1 h after a glucose load [17 (link),18 (link)]. Briefly, 15 amino acids, 45 acylcarnitines and 5 conventional clinical and targeted metabolites were analyzed. Levels of conventional clinical metabolites, including lactate, triglycerides, 3-hydroxybutyrate, glycerol, and nonesterified fatty acids (NEFA), were measured on a Unicel DxC 600 clinical analyzer (Beckman Coulter, Brea, CA, USA). Targeted metabolomics assays for acylcarnitines and amino acids were conducted by tandem mass spectrometry (MS) with addition of known quantities of stable isotope-labeled internal standards on an Acquity TQD Triple Quadrupole system (Waters Corporation, Milford, MA, USA).
Acquity tqd triple quadrupole system
Manufactured by Waters Corporation
Sourced in United States
The Acquity TQD Triple Quadrupole system is a highly sensitive and selective mass spectrometry instrument designed for analytical applications. It consists of two quadrupole mass filters and a collision cell, allowing for the detection and quantification of target analytes with high precision and accuracy.
Automatically generated - may contain errors
Lab products found in correlation
5 protocols using acquity tqd triple quadrupole system
1
Metabolite Profiling in Maternal Serum
2
Comprehensive Metabolite Profiling Protocol
3
Quantitative Metabolite Profiling Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Sixty-two conventional clinical and targeted metabolites were quantitatively assayed, as previously described [15 (link)]. Briefly, levels of conventional clinical metabolites (lactate, triglycerides, 3-hydroxybutyrate, glycerol, and NEFA) were measured using a Unicel DxC 600 clinical analyzer (Beckman Coulter, Brea, CA, USA). Targeted metabolomic assays for acylcarnitines and amino acids were performed via tandem mass spectrometry (MS), for which known quantities of stable isotope-labelled internal standards were added using an Acquity TQD Triple Quadrupole system (Waters Corporation, Milford, MA, USA).
4
Quantifying Metabolites and Amino Acids
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Conventional metabolites were measured on a Beckman-Coulter DxC600 autoanalyzer using reagents from Beckman (Brea, CA; lactate) and Wako USA (Richmond, VA; beta-hydroxybutyrate). For free glycerol, reagents by Roche (Indianapolis, IN) for glycerol-blanked triglycerides were modified. To 84 mL of the Roche R1 reagent, 6.0 mg 4-aminoantipyrine dye (Sigma, St. Louis, MO) was added. This assay was run by combining 250 μL reagent with 20 μL sample volume, calibrated against a glycerol standard (2.29 mM) with detection at 520 nm after 5 min. Targeted assays of amino acids using stable-isotope-labeled internal standards were performed on an Acquity TQD Triple Quadrupole system (Waters Corporation, Milford, MA). Absolute metabolite concentrations were calculated based on the linear relationship between concentration and peak area, and calibrated against internal standards with known concentrations, as previously described [34 (link), 35 (link)]. Conventional metabolite and targeted amino acid data are used here to evaluate whether each normalization method can improve correlation of non-targeted GC/MS data with targeted measurements that are quantified using internal standards and not subject to batch effects.
5
Conventional and Targeted Metabolomics Assays
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Conventional clinical metabolite and targeted metabolomics assays were conducted as previously described (18 (link)). In brief, conventional metabolites (lactate, triglycerides, β-hydroxybutyrate, glycerol, and nonesterified fatty acids [NEFA]) were measured on a Beckman-Coulter Unicell DxC 600 clinical analyzer. Targeted metabolomics assays for acylcarnitines and amino acids used tandem mass spectrometry with addition of known quantities of stable isotope-labeled internal standards on an Acquity TQD Triple Quadrupole system (Waters Corporation, Milford, MA). Sixty-two conventional and targeted metabolites were analyzed.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!