Cell cycle analysis was performed according to BD Pharmingen™ BrdU Flow Kit (BD Biosciences, San Jose, CA USA) and conducted on HCT116 cells (1.5 × 105 cells seeded in a 6-well plate), overnight serum deprived and treated or not with um-PEA (30 μM) for 24 h. Cells were revealed by using BriCyte flow cytometer (Mindray, Trezzano sul Naviglio Italy), gated based on forward and side scatter to separate debris, and then the cellular events were further gated based on their BrdU and 7-AAD content. Data were analyzed by FlowJo v10 software (Tree Star, Ashland, OR, USA) and expressed as fold change of the cells in each cell cycle phase.
Bricyte flow cytometer
Manufactured by Mindray
Sourced in Italy
The BriCyte flow cytometer is a compact, versatile instrument designed for clinical and research applications. It offers multiparameter analysis of cells, particles, and cellular components. The BriCyte utilizes advanced fluidics and optics to enable high-throughput, accurate, and reproducible measurements.
Automatically generated - may contain errors
3 protocols using bricyte flow cytometer
1
Cell Cycle Analysis of HCT116 Cells
2
Cell Cycle Analysis of HCT116 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell cycle analysis was performed according to BD Pharmingen™ bromodeoxyuridine Flow Kit (BD Biosciences, Milan, Italy) and conducted on HCT116 cells and/or siNAAA‐HCT116 (1.5 × 105 cells seeded in a six‐well plate), overnight serum deprived and treated or not with AM9053 (3 μM) for 24 h. Cells were revealed by using BriCyte flow cytometer (Mindray, Trezzano sul Naviglio, Italy), gated based on forward and side scatter to separate debris, and then the cellular events were further gated based on their bromodeoxyuridine and 7‐aminoactinomycin D content. Data were analysed by FlowJo v10 software (Tree Star, Ashland, OR, USA) and expressed as fold change of the cells in each cell cycle phase.
3
Quantifying Apoptosis in MDA-MB-231 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Apoptosis was measured using the BD Pharmingen™ FITC Annexin V Apoptosis Detection Kit I (cat. 556547, BD Biosciences, Franklin Lakes, NJ, USA) according to the manufacturer’s instructions. MDA-MB-231 cells were seeded (2.5 × 105 cells/well) in 35 mm culture dishes. The day next, the cells were either treated with erucin (30 μM) for 48 h or left untreated. MDA-MB-231 cells were later collected and stained with Annexin V-FITC/Propidium Iodide (PI). Flow cytometry analysis was performed using a BriCyte flow cytometer (Mindray, Italy). A minimum of 50,000 events for each sample were collected and data were analyzed using FlowJo v10 software (Tree Star, Ashland, OR USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!