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Anti hnrnpa1

Manufactured by Abcam
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Anti-HNRNPA1 is a primary antibody that recognizes the HNRNPA1 protein. HNRNPA1 is a member of the heterogeneous nuclear ribonucleoprotein (hnRNP) family and plays a role in various cellular processes, including mRNA splicing and transport.

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Lab products found in correlation

Anti tubulin, by Merck Group (2 mentions) Anti actin, by Santa Cruz Biotechnology (2 mentions) Bca protein assay kit, by Thermo Fisher Scientific (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions) Rnaimax, by Thermo Fisher Scientific (2 mentions) Anti hnrnpa2b1, by Abcam (1 mentions) Rna immunoprecipitation kit, by Geneseed (1 mentions) Anti tpp1, by Abcam (1 mentions) Anti trf2, by Merck Group (1 mentions) Anti nucleolin, by Abcam (1 mentions) Anti histone h2b, by Merck Group (1 mentions) Anti histone h3, by Abcam (1 mentions) Anti gapdh, by Merck Group (1 mentions) Normal rabbit igg, by Santa Cruz Biotechnology (1 mentions) Anti α tubulin, by Merck Group (1 mentions) Anti her2, by Cell Signaling Technology (1 mentions) Anti jag1, by Cell Signaling Technology (1 mentions) Bis tris gradient gel, by Thermo Fisher Scientific (1 mentions) Anti msi2, by Abcam (1 mentions) Anti hnrnp a2 b1, by Santa Cruz Biotechnology (1 mentions)

Close spelling variants of this product

HnRNPA1 (15 citations) Anti-hnRNPA1 antibody (3 citations)

8 protocols using anti hnrnpa1

1

Mapping HNRNPA1 and HNRNPA2/B1 Interactions

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The interactions between HNRNPA1 and miR-483-5p, HNRNPA2/B1, and miR-483-5p were assessed using an RNA immunoprecipitation kit (Geneseed Biotech). Specifically, the primary mouse renal TECs were lysed and then incubated with an RIP buffer containing anti-HNRNPA1 (Abcam) and anti-HNRNPA2/B1 (Abcam) conjugated magnetic beads. The expression of miR-483-5p was tested by qRT-PCR.
Liu D., Liu F., Li Z., Pan S., Xie J., Zhao Z., Liu Z., Zhang J, & Liu Z. (2021). HNRNPA1-mediated exosomal sorting of miR-483-5p out of renal tubular epithelial cells promotes the progression of diabetic nephropathy-induced renal interstitial fibrosis. Cell Death & Disease, 12(3), 255.
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2

ChIP-seq Antibodies and Immunoblotting Protocols

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For ChIP, normal rabbit IgG (Santa Cruz, sc-2027), anti-histone H3 (Abcam, ab1791), anti-TRF1 (in-house), anti-TRF2 (Millipore, #05-521), anti-TPP1 (in-house) and anti-Pol II C-terminus domain (CTD) (Abcam, ab5408; clone 4H8) were used. Anti-phospho Ser-2 CTD (clone 3E10) and anti-phospho Ser-5 CTD were previously described [27] (link). Immunoblotting utilized anti-nucleolin (Abcam, ab13541), anti-tubulin (Sigma, T3526), anti-hnRNPA1 (Abcam, ab10685) anti-histone H2B (Millipore, #07-371), and anti-GAPDH (Millipore, clone 6C5).
Wakai M., Abe S., Kazuki Y., Oshimura M, & Ishikawa F. (2014). A Human Artificial Chromosome Recapitulates the Metabolism of Native Telomeres in Mammalian Cells. PLoS ONE, 9(2), e88530.
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3

Western Blotting and Immunofluorescence of Murine Neural Stem Cells

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For western blotting, cells were lysed on ice and protein lysates were loaded onto
4-12% gradient Bis-Tris Gel (Life Technologies). Primary antibodies and dilutions
used in western blotting on murine NSCs: anti-MSI1/2 (Cell Signaling Technology
#2154, 1:800), anti-MSI2 (Abcam #57341, 1:800), anti-Jag1 (Cell Signaling Technology
#2620, 1:800), anti-HER2 (Cell Signaling Technologies #2248, 1:1000), anti-phos-HER2
(Cell Signaling Technology #2241, 1:1000), anti-alpha-Tubulin (Sigma-Aldrich T9026,
1:5000), anti-HNRNPA1 (Abcam ab5832, 1:800). Immunofluorescene was performed on cells
grown on glass bottom chambers (LabTek II, #1.5), fixed in 4% PFA. Cells were blocked
and permeabilized in 5% FBS, .1% Triton in PBS(+). Antibodies were applied in 1%
FBS in PBS(+). Immunofluorescence antibodies and dilutions: anti-MSI1 (MBL
D270-3, 1:500), anti-HNRNP A2/B1 (Santa Cruz, sc-374052, 1:200). For IHC on murine
mammary glands, anti-Jag1 (Santa Cruz, SC-6011, 1:100) was used. For western on
murine mammary glands, anti-Jag1 (Santa Cruz, SC-6011, 1:1000) and anti-Tubulin
(Sigma-Aldrich, T5168, 1:4000) were used.
Katz Y., Li F., Lambert N.J., Sokol E.S., Tam W.L., Cheng A.W., Airoldi E.M., Lengner C.J., Gupta P.B., Yu Z., Jaenisch R, & Burge C.B. (2014). Musashi proteins are post-transcriptional regulators of the epithelial-luminal cell state. eLife, 3, e03915.
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4

Comprehensive Western Blot Analysis of Key Proteins

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The specific primary antibodies involved in western blot(WB) analysis were as follows: anti- VE-cadherin (1:1000), anti-ZO-1 (1:1000), anti-TSG101(1:1000, Abcam, UK), anti-VEGFR-2 (1:1000, Abcam, UK), anti-Fas (1:1000, Abcam, UK), anti-p53 (1:1000), anti-HIPK3 (1:1000), anti-Caspase-3(1:1000, Abcam, UK), anti-HNRNPA1(1:1000), anti-HIF-1α(1:1000), anti-DDDDK tag(1:1000), anti-CD9 (1:1000, Abcam, UK), anti-CD63(1:1000, Abcam, UK), anti-ALIX(1:1000, Abcam, UK), anti-β-actin(1:1000, Proteintech, Wuhan, China), anti-SFRS2(1:1000, Abcam, UK), anti-ACO-1(1:1000, Abcam, UK). The secondary antibody was HRP-IgG (1:10000, Proteintech, Wuhan, China) and β-actin was used as internal reference.
Yang X., Wu M., Kong X., Wang Y., Hu C., Zhu D., Kong L., Qiu F, & Jiang W. (2024). Exosomal miR-3174 induced by hypoxia promotes angiogenesis and metastasis of hepatocellular carcinoma by inhibiting HIPK3. iScience, 27(2), 108955.
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5

Western Blot Analysis of HNRNPA1 Protein

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Cells were lysed in RIPA buffer with 1mM PMSF. The concentration of the total protein obtained was quantified using a BCA protein assay kit (Thermo). Proteins were separated on 10% SDS‐PAGE and electro‐transferred to the PVDF membrane (Millipore). And then were incubated with anti‐HNRNPA1 (Abcam) and anti‐β‐actin (Abcam) at 4°C overnight, then incubated with an HRP‐labelled secondary antibody IgG (Abcam) at room temperature for 1h. Immunolabeling was visualized using the ECL system (Millipore).
Chen F., Sha S., Wang S., Shi H., Dong L., Liu D., Cheng Y., An M., Wang Y, & Zhang J. (2020). RP11‐81H3.2 promotes gastric cancer progression through miR‐339‐HNRNPA1 interaction network. Cancer Medicine, 9(7), 2524-2534.
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6

Antibody Characterization Protocol

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The following antibodies were used: anti-LYVE-1, Abcam (ab14917), for IHC; anti-F4/80, Abcam (ab6640), for IHC; anti-hnRNPL, Abcam (ab6106), for immunoblot, RIP and ChIP; anti-hnRNPQ, Abcam (ab184946), for immunoblot; anti-hnRNPA1, Abcam (ab4791), for immunoblot; anti-hnRNPL, Abcam (ab133607), for immunoblot; anti-CD68, Cell Signaling Technology, #76437, for IHC and flow cytometry; anti-CD206, Abcam (ab64693), for flow cytometry; anti-CCL2, Abcam (ab9669), for immunoblot and IHC; CCL2-neutralizing antibody (#554440, BD Biosciences, NJ, USA); anti-GAPDH, Cell Signaling Technology, #5174, for immunoblot; anti-H3K4me3, Abcam (ab8580), for immunoblot and ChIP; anti-VEGF-C, Abcam (ab9546), for immunoblot and IHC; and VEGF-C-neutralizing antibody (pV1006R-r, Angio-Proteomie, Boston, MA, USA). Control mouse IgG, control rabbit IgG, anti-RNA pol II, and anti-snRNP70 were provided in the EZ-Magna RIP kit or EZ-Magna ChIP A/G kit (Millipore). DAPI (Thermo Scientific, 62247) and Alexa Fluor™ 555 Phalloidin (Invitrogen™) were also used.
Chen C., He W., Huang J., Wang B., Li H., Cai Q., Su F., Bi J., Liu H., Zhang B., Jiang N., Zhong G., Zhao Y., Dong W, & Lin T. (2018). LNMAT1 promotes lymphatic metastasis of bladder cancer via CCL2 dependent macrophage recruitment. Nature Communications, 9, 3826.
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7

C/EBPγ Knockdown in Cell Lines

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Cells were transfected with siRNA oligos synthesized by IDT for each gene using RNAi Max (Thermo Fisher Scientific) according to the manufacturer's protocol. The following sequences were used for the knockdown (KD) of Cebpg transcript: 5′- GAT ACA CTG CAA AGA GTA AAC CAG C-3′ (Cebpg siRNA). The antibodies used for western analysis are as follows: anti-hnRNP A1 (4B10 from Abcam), anti-CPSF3 (sc-393001, Santa Cruz Biotechnology), anti-CPSF4 (sc-393316, Santa Cruz Biotechnology), CPSF7 (sc-393880, Santa Cruz Biotechnology), CstF1 (sc-393260, Santa Cruz Biotechnology), anti-CPSF6 (ab175237 from Abcam), anti-Tubulin (sc-53646, Santa Cruz Biotechnology), anti-C/EBPγ (sc-517003, Santa Cruz Biotechnology), anti-Lamin A/C (#4777 from Cell Signaling), anti-ATF4 (sc-390063, Santa Cruz Biotechnology), anti-Actin (ac-47778, Santa Cruz Biotechnology), anti-S6 (#2317 from Cell Signaling), and anti-pS6 (#2211 from Cell Signaling).
Chang J.W., Zhang W., Yeh H.S., Park M., Yao C., Shi Y., Kuang R, & Yong J. (2018). An integrative model for alternative polyadenylation, IntMAP, delineates mTOR-modulated endoplasmic reticulum stress response. Nucleic Acids Research, 46(12), 5996-6008.
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8

Western Blotting of Cell Lysates

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Lysate from cell lines and primary cells were subjected to SDS-PAGE and Western blotting as described previously (46 (link)). The antibodies used were anti-actin, anti-Myc, anti-C/EBPβ, anti-Alix, anti-Twist, and anti-CD63 (Santa Cruz Biotechnology); anti-GRB2 (Transduction Laboratories); anti-JAK2, anti-β-catenin, anti-FoxM1, anti-CDK6, anti–pJAK2Y1007/1008, and anti-CCND2 (Cell Signaling Technology); anti-SET (Globozymes); anti-ABL (Ab-3), anti-CCND1/2, anti-PY (4G10), and anti-PP2AcY307 (EMD); anti-hnRNPA1 (Abcam); and anti-Flag (M2; Sigma).
Silvestri G., Trotta R., Stramucci L., Ellis J.J., Harb J.G., Neviani P., Wang S., Eisfeld A.K., Walker C.J., Zhang B., Srutova K., Gambacorti-Passerini C., Pineda G., Jamieson C.H., Stagno F., Vigneri P., Nteliopoulos G., May P.C., Reid A.G., Garzon R., Roy D.C., Moutuou M.M., Guimond M., Hokland P., Deininger M.W., Fitzgerald G., Harman C., Dazzi F., Milojkovic D., Apperley J.F., Marcucci G., Qi J., Polakova K.M., Zou Y., Fan X., Baer M.R., Calabretta B, & Perrotti D. (2020). Persistence of Drug-Resistant Leukemic Stem Cells and Impaired NK Cell Immunity in CML Patients Depend on MIR300 Antiproliferative and PP2A-Activating Functions. Blood Cancer Discovery, 1(1), 48-67.
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