Ar sc 816

Manufactured by Santa Cruz Biotechnology

Sourced in United States

AR (sc-816) is a laboratory product manufactured by Santa Cruz Biotechnology. It is an antibody used for research purposes. The core function of this product is to detect and analyze the presence of the androgen receptor (AR) protein in biological samples.

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Lab products found in correlation

Dab kit, by Vector Laboratories (4 mentions) Ap conjugated anti α smooth muscle actin antibody, by Merck Group (4 mentions) Sigmafast fast red tr naphthol as mx tablets, by Merck Group (4 mentions) Abc elite kit, by Vector Laboratories (4 mentions) Flag tag (flag), by Merck Group (2 mentions) Huwe1 a300 486a, by Fortis Life Sciences (2 mentions) Ubiquitin sc 9133, by Santa Cruz Biotechnology (2 mentions) Jmjd1a a301 539a or 538b, by Fortis Life Sciences (2 mentions) Ar 06 680 or h3k9me2 07 441, by Merck Group (2 mentions) 7425 and actin a5441, by Merck Group (2 mentions) Cleaved caspase 3, by Cell Signaling Technology (2 mentions) Ccd camera, by Olympus (2 mentions) Mmp2 nb200 193, by Novus Biologicals (2 mentions) Pakt t308, by Cell Signaling Technology (2 mentions) Mmp 9, by Abcam (2 mentions) P akt s473, by Santa Cruz Biotechnology (2 mentions) Pcna sc 56, by Santa Cruz Biotechnology (2 mentions) Atf3 sc188, by Santa Cruz Biotechnology (2 mentions) P63 sc 8430, by Santa Cruz Biotechnology (2 mentions) Ck5 prb 160p, by BioLegend (2 mentions)

Close spelling variants of this product

Sc-816 (53 citations) Anti-AR (sc-816, (9 citations) AR (N-20)(sc-816, (9 citations) Anti-AR (N-20) (sc-816, (4 citations) AR N20 antibody (sc-816, (2 citations) Anti-AR (N-20 (Catalog #sc-816) and 441 (Catalog #sc-7305), (2 citations)

16 protocols using ar sc 816

Antibody Validation for Cell Analysis

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Antibodies to AR (sc-816), ubiquitin (sc-9133), HA (sc-805), GFP (sc-8334 or sc-9996), myc (sc-789), tubulin (sc-8035) (all from Santa Cruz), JMJD1A (A301-539A or 538B) or HUWE1 (A300-486A) (Bethyl Laboratories), AR (06-680) or H3K9me2 (07-441) (EMD Millipore), c-Myc (9402), p53 (9282), PARP (9542), active caspase-3 (9661) (all from Cell Signaling), and Flag (F7425) and actin (A5441) (Sigma) were used according to the manufacturers’ recommendations.

Fan L., Peng G., Sahgal N., Fazli L., Gleave M., Zhang Y., Hussain A, & Qi J. (2015). Regulation of c-Myc expression by the histone demethylase JMJD1A is essential for prostate cancer cell growth and survival. Oncogene, 35(19), 2441-2452.

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Immunohistochemical Analysis of Prostate Lobes

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Prostate lobes were separated by microdissection, and embedded in paraffin for sectioning ^{24 (link)}. After antigen retrieval in hot citrate buffer, sections were blocked in 5% of normal horse serum and 1% of normal goat serum, and subjected to immunohistochemistry staining using the ABC Elite Kit and the DAB Kit (Vector) according to the manufacturers’ recommendations. The following antibodies were used: Ki-67 (ab15580, 1:600) from Abcam; ATF3 (Santa Cruz sc-188, 1:200), AR (sc-816, 1:200), and p63 (sc-8430, 1:200) from Santa Cruz; p-AKT S473 (#4060, 1:200), p-AKT T308 (#2965,1:200), AKT (#4691, 1:200), p-S6 (#2211, 1:400), S6 (#2217, 1:200), cleaved caspase-3 (#9661, 1:300), and Pten (#9188, 1:100)from Cell Signaling; MMP2 (NB200-193, 1:200) from Novus; and MMP-9 (ab137867, 1:1000) from Abcam. For α-smooth muscle actin (α-SMA) staining, sections were incubated with alkaline phosphatase (AP)-conjugated anti-α-smooth muscle actin antibody (Sigma, 1:600) followed by detection of AP activity using the SIGMAFAST Fast Red TR/Naphthol AS-MX tablets (F4523, Sigma) according to the supplier’s protocol. For quantifying IHC staining intensity, random microscopic fields were captured and digitized by a CCD camera (Olympus). Signal intensity was determined using the Image-Pro Plus software and presented as integrated optical density (IOD).

Wang Z., Xu D., Ding H.F., Kim J., Zhang J., Hai T, & Yan C. (2014). Loss of ATF3 Promotes Akt Activation and Prostate Cancer Development in a Pten Knockout Mouse Model. Oncogene, 34(38), 4975-4984.

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Immunohistochemical Analysis of Cell Markers

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Immunohistochemical studies were conducted as previously described [26 (link)]. Antihuman polyclonal rabbit IgG served as a negative control. The antibodies used were Ki67 (MIB-1,DAKO; dilution 1:100), p53 (sc-126, Santa Cruz Biotechnology, dilution 1:200), MDM2 (18–2403, Clone IF2, Invitrogen; dilution 1:25), MDM4 (MDMX Abcam 76362; dilution 1:2000), p14ARF (clone 4C6/4 MAB 3782; Chemicon International; dilution 1:250), AR (sc-816, Santa Cruz Biotechnology, dilution 1:500). The degree of staining was evaluated blindly in a semiquantitative fashion by a pathologist (F.U.M. and A.D.B.) noting both the intensity of staining as well as the percentage of cells exhibiting that intensity in the nucleus and the cytoplasm. The intensity of the staining was scored as no staining (0), weak (1), intermediate (2), and strong (3) staining. The composite score was the number of cells staining multiplied by the intensity of staining.

Siddiqui S., Libertini S.J., Lucas C.A., Lombard A.P., Baek H.B., Nakagawa R.M., Nishida K.S., Steele T.M., Melgoza F.U., Borowsky A.D., Durbin-Johnson B.P., Qi L., Ghosh P.M, & Mudryj M. (2020). The p14ARF tumor suppressor restrains androgen receptor activity and prevents apoptosis in prostate cancer cells. Cancer letters, 483, 12-21.

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Protein Expression Analysis in Rat Prostate

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Antibodies against inducible nitric oxide (NO) synthase (iNOS; sc-650), cyclooxygenase-2 (COX-2; sc-1745), Bax (sc-7480), Bcl-xL (sc-8392), Caspase-3 (sc-7272), Caspase-8 (sc-5263), Proliferating cell nuclear antigen (PCNA; sc-56), androgen receptor (AR; sc-816), and β-actin (sc-81178) were purchased from Santa Cruz Biotechnology, Inc. (TX, USA). Antibody against prostate-specific antigen (PSA; PB9259) was procured from Boster Biological Technology (CA, USA). Antibodies against 5' adenosine monophosphate-activated protein kinase (AMPK; #2532) and p-AMPK (#2535) were obtained from Cell Signaling Technology (MA, USA). Total protein was extracted from prostatic tissues of rats and western blot analysis was performed as described earlier [51 (link)].

Jin B.R, & An H.J. (2020). Baicalin alleviates benign prostate hyperplasia through androgen-dependent apoptosis. Aging (Albany NY), 12(3), 2142-2155.

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Investigating DNA Damage Response Signaling

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AR (sc-816) from Santa Cruz; c-Myb (ab109127), BRCA1 (ab16780) and TopBP1 (ab2402) from Abcam; GAPDH (#21188), ATR (#2790), P-ATR (Ser⁴²⁸) (#2853), Chk1 (#2360), P-Chk1 (Ser³¹⁷) (#12302), P-Chk1 (Ser³⁴⁵) (#2348) from Cell Signaling.

Li L., Chang W., Yang G., Ren C., Park S., Karantanos T., Karanika S., Wang J., Yin J., Shah P.K., Takahiro H., Dobashi M., Zhang W., Efstathiou E., Maity S.N., Aparicio A.M., Tapia E.M., Troncoso P., Broom B., Xiao L., Lee H.S., Lee J.S., Corn P.G., Navone N, & Thompson T.C. (2014). Targeting Poly (ADP-Ribose) Polymerase and the c-Myb-TopBP1-ATR-Chk1 Signaling Pathway in Castration-Resistant Prostate Cancer. Science signaling, 7(326), ra47.

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Protein Extraction and Immunoblotting Protocol

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Cells were lysed in RIPA buffer (150mM NaCl, 50mM Tris-HCl, 1% NP-40, 0.1%SDS, 0.5% Sodium Deoxycholate, pH8.0). Antibodies used included: Hsp70 (sc-1060, Santa Cruz Biotechnology, Dallas, TX, USA), AR (sc-816, Santa Cruz Biotechnology), γ-tubulin (sc-17787, Santa Cruz Biotechnology), GAPDH (FL-335, sc-25778, Santa Cruz Biotechnology), and secondary antibody IgG-HRP (sc-2004 sc-2005, Santa Cruz Biotechnology). Hsp90 (SPA-835-D), Hsp40 (SPA-400-D), HOP (SRA-1500-D), and Hsp27 (SPA-800D) antibodies were from Stressgen Bioreagents (Victoria, BC, CA); Flag antibody (F1804, St. Louis, MO, USA) was from SIGMA-ALDRICH and GFP antibody (TP401, Houston, TX, USA) was from Torrey Pines Biolabs. Most of the Western blotting membranes were developed by films. Some were observed by VersaDoc imaging system (4000 MP, Bio-Rad, Hercules, CA, USA) following the manufacture’s instruction. Densitometry was performed using Image J software (36 (link)). Band intensities were normalized to GAPDH or γ-tubulin individually.

Dong J., Wu Z., Wang D., Pascal L.E., Nelson J.B., Wipf P, & Wang Z. (2018). Hsp70 binds to the androgen receptor N-terminal domain and modulates the receptor function in prostate cancer cells. Molecular cancer therapeutics, 18(1), 39-50.

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Antibody Validation for Cell Analysis

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Prostate Immunohistochemical Staining Protocol

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These were carried out essential as described previously^{13 (link)}. In brief, prostate sections were treated with a hot citrate buffer, and subjected to IHC staining using the ABC Elite Kit and the DAB Kit (Vector laboratories, Burlingame, CA, USA) according to the manufacturers’ protocols. PCNA (sc-56, 1:1000), ATF3 (sc-188, 1:200), AR (sc-816, 1:200), and p63 (sc-8430, 1:200) antibodies were purchased from Santa Cruz (Dallas, TX, USA). α-smooth muscle actin (α-SMA) staining were performed using an alkaline phosphatase (AP)-conjugated anti-α-smooth muscle actin antibody (Sigma, 1:600) and SIGMAFAST Fast Red TR/Naphthol AS-MX tablets (F4523, Sigma-Aldrich, St Louis, MO, USA) according to the supplier’s protocol. For CK5/CK8 double staining, prostate sections were incubated with CK5 (PRB-160P, 1:500) and CK8 (MMS-162P, 1:500) antibodies (Biolegend, San Diego, CA, USA), followed by incubation with Alexa Fluor 594-conjugated anti-rabbit IgG (A-24923) and Alexa Fluor 488-conjugated anti-mouse IgG (A-21131, Life Technologies).

Wang Z., Kim J., Teng Y., Ding H.F., Zhang J., Hai T., Cowell J.K, & Yan C. (2015). Loss of ATF3 Promotes Hormone-induced Prostate Carcinogenesis and the Emergence of CK5+CK8+ Epithelial Cells. Oncogene, 35(27), 3555-3564.

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Antibody Panel for Protein Analysis

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The following antibodies were used: SPOP (ab137537; Abcam), SPOP (16750-1-AP; proteintech), INF2 (20466-1-AP; proteintech), AR (SC-816; Santa Cruz), DEK (16448-1-AP, Proteintech), IQGAP1(ab133490; Abcam), DRP1(8570S; CST), KPNA5(A7731; Abcam), COX4 (Abcam; ab14744), Ubiquitin (6652–1; epitomics), Myc (9E10; Sigma), FLAG (M2; Sigma), HA (MM5-101R; Convance), Actin (AC-74; Sigma). Mdivi-1 was purchased from Selleckchem. MitoSOX Red dye was purchased from Invitrogen.

Jin X., Wang J., Gao K., Zhang P., Yao L., Tang Y., Tang L., Ma J., Xiao J., Zhang E., Zhu J., Zhang B., Zhao S.M., Li Y., Ren S., Huang H., Yu L, & Wang C. (2017). Dysregulation of INF2-mediated mitochondrial fission in SPOP-mutated prostate cancer. PLoS Genetics, 13(4), e1006748.

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Western Blot Analysis of Protein Modifications

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Whole-cell or immunoprecipitated protein extracts were denatured, resolved on SDS-PAGE gels and transferred onto PVDF membranes (BioRad) using a wet transfer apparatus. After blocking, blots were probed overnight and the following primary antibodies were used: AR (sc-816), Ubiquitin (sc-8017) and GAPDH (sc-8017) from Santa Cruz Biotechnology, AR441 (MA5–13426) and Hsp27 (MA3–015) from ThermoFisher, SUMO1 (49,305, Cell Signaling), SUMO2/3 (ab81371, Abcam), PIAS1 (49,305, Cell Signaling), and β-actin (Sigma-Aldrich). Antigen–antibody complexes were then detected by the chemiluminescence Western Lighting Plus-ECL reagent (PerkinElmer).

Bahnassy S., Thangavel H., Quttina M., Khan A.F., Dhanyalayam D., Ritho J., Karami S., Ren J, & Bawa-Khalfe T. (2020). Constitutively active androgen receptor supports the metastatic phenotype of endocrine-resistant hormone receptor-positive breast cancer. Cell Communication and Signaling : CCS, 18, 154.

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