RNA was extracted from homogenized tissues using TRIzol (Sigma) reagent according to the manufacturer’s protocol and concentration was measured using a Nanodrop One UV spectrophotometer (Thermo Fisher Scientific). For mouse plasma RNA extraction, TRIzol LS (Thermo Fisher Scientific) reagent was mixed with 50 μl of plasma samples along with 9.9 amol of Caenorhabditis elegans mir-39 (cel-mir-39) as a spike-in control and processed according to the manufacturer’s protocol. To improve RNA recovery yield, 5 μg of glycogen (Invitrogen) was added to samples during alcohol precipitation and chilled at −20°C for 18h. For human plasma RNA extraction, miRNeasy Serum/Plasma advanced kit (Qiagen) was used to extract RNA from 250 μl EDTA anti-coagulated plasma following the manufacturer’s instruction. The purified RNA pellets were then resuspended in DEPC H2O and quantified with fluorometric Quant-it RNA Assay Kit (Thermo Fisher Scientific). Alternatively, the Bioanalyzer 2100 Small RNA Kit (Agilent Technologies) was used to quantify small RNA.
Bioanalyzer 2100 small rna kit
Manufactured by Agilent Technologies
Sourced in United States
The Bioanalyzer 2100 Small RNA Kit is a tool designed for the analysis of small RNA samples, including microRNA (miRNA), small interfering RNA (siRNA), and other short RNA fragments. The kit provides a rapid and sensitive method for the assessment of RNA quality and quantity, allowing users to obtain detailed information about the size distribution and concentration of small RNA molecules within a sample.
Automatically generated - may contain errors
Lab products found in correlation
Trizol ls, by Thermo Fisher Scientific (2 mentions)
Rnase free dnase set, by Qiagen (2 mentions)
Mirneasy mini kit, by Qiagen (2 mentions)
Nanodrop spectrophotometer, by Thermo Fisher Scientific (2 mentions)
Glycogen, by Thermo Fisher Scientific (1 mentions)
Quant it rna assay kit, by Thermo Fisher Scientific (1 mentions)
Nanodrop one uv spectrophotometer, by Thermo Fisher Scientific (1 mentions)
Mirneasy serum plasma advanced kit, by Qiagen (1 mentions)
Trizol, by Merck Group (1 mentions)
Mirneasy mini, by Qiagen (1 mentions)
Rneasy minelute, by Qiagen (1 mentions)
4 protocols using bioanalyzer 2100 small rna kit
1
RNA Extraction from Plasma and Tissues
2
Small RNA Extraction from Endometrial Tissue
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Up to 30 mg of the endometrial tissue was processed using miRNeasy Mini and RNeasy MinElute kits (Qiagen), following the manufacturer’s protocol for isolating small RNA (<200 nucleotides) separately from large RNA molecules. DNase I treatment was performed on column using RNase-Free DNase Set (Qiagen). Purified RNA quantity was determined with Bioanalyzer 2100 Small RNA kit (Agilent Technologies, Santa Clara, CA, USA).
3
RNA Extraction and Characterization
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BmN4 or High Five cells were harvested by means of a cell scraper, pelleted and snap frozen. For qRT-PCR, the RNA was extracted with the miRNeasy Mini Kit (Qiagen, Hilden, Germany), as described in the correspondent protocol. A DNase treatment (RNase-free DNase set, Qiagen, Hilden, Germany) was performed to eliminate potential genomic DNA contamination. Quality and concentration of the extracted RNA were assessed using a Nanodrop spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA). For sRNA sequencing, sRNA extractions were performed with the miRNeasy Mini Kit (Qiagen, Hilden, Germany) according to the manufacturers’ protocol for sRNAs. Quality and concentration of the extracted sRNA molecules were assessed with the 2100 Bioanalyzer Small RNA kit (Agilent, Santa Clara, CA, USA).
4
Extraction and Analysis of sRNA
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Small (s)RNA extractions were performed with the miRNeasy Mini kit (Qiagen, Hilden, Germany) according to the manufacturer’s protocols. Quality and concentration of the extracted sRNA molecules were assessed with the 2100 Bioanalyzer Small RNA kit (Agilent Technologies, Leuven, Belgium). Samples were pooled prior to sequencing and stored at −80 °C until further processing.
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