Tguide cell tissue genomic dna extraction kit osr m401

In this paper, high-throughput sequencing technology was used. Genomic DNA was extracted from a specimen muscle using the Tguide Cell/tissue genomic DNA Extraction Kit (OSR-M401) (Tiangen, Beijing, China). The main experimental steps are as follows: (1) DNA sample quality control; (2) DNA Library Construction; (3) PCR Amplification; (4) Size Selection; (5) Library Quality Check; (6) Library Pooling and Sequencing, the amplified original library was submitted to Illumina HiSeq 4000 Sequencing platform (Illumina, CA, USA).

The muscle tissue was collected for genomic DNA isolation using the Tguide Cell/tissue genomic DNA Extraction Kit (OSR-M401) (Tiangen, Beijing, China). After DNA sample quality control, a DNA library was constructed and amplified by PCR, followed by size selection and library quality check, finally the amplified original library DNA was subjected to Illumina HiSeq 4000 Sequencing platform (Illumina, CA). The sequenced fragments were processed for the quality check to filtrate low-quality reads on FastQC Version 0.11.8 (Andrews 2015 ), and then assembled into a circular mitogenome of B. barila by MetaSPAdes 3.13.0 (Nurk et al. 2017 (link)) with Barilius malabaricus MN650735 as reference, and then the assembled mitochondrial genome sequences were annotated using MitoMaker 1.14 (Bernt et al. 2013 (link)) with default parameters. The resulting complete mitochondrial genome was deposited in GenBank with accession number OM617728. OGDRAW (https://chlorobox.mpimp-golm.mpg.de/OGDraw.html) was used to draw the genome maps (Greiner et al. 2019 (link)). Alignments, analysis, model calculation, and phylogenetic reconstruction were all completed by MEGA X (Kumar et al. 2018 (link)).