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Snu 449 cells

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SNU-449 cells are a human liver cancer cell line derived from a hepatocellular carcinoma sample. The cells are adherent in nature and maintain an epithelial-like morphology. SNU-449 cells can be used for various in vitro studies related to liver cancer research.

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2 protocols using snu 449 cells

1

Culturing Hepatocellular Carcinoma Cell Lines

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Hep3B, HCCLM3, SK-Hep1 and 293 T cells were obtained from the Cell Bank Type Culture Collection of the Chinese Academy of Sciences, Shanghai, China. SNU-449 cells were purchased from ATCC (Manassas, VA, USA). Hep3B cells were cultured in Minimum Essential Medium (HyClone, Logan, UT, USA) containing 10% FBS (HyClone), 1% penicillin/streptomycin (HyClone) at 37 °C and 5% CO2. All other cells were cultivated in Dulbecco’s modified Eagle’s medium (HyClone, Logan, UT, USA) containing 10% FBS (HyClone), 1% penicillin/streptomycin at 37 °C and 5% CO2.
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2

Hepatocellular Carcinoma Cell Lines

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Hepatocellular carcinoma cell line Hep3B (CL‐0102) and human hepatic stellate cell line LX‐2 (CL‐0560) were purchased from Procell. Huh7 cells were purchased from the Chinese Academy of Sciences Shanghai Cell Bank. SNU‐449 cells were purchased from the ATCC. LX‐2, SNU‐449, and Huh7 cells were cultured in DMEM medium (Gibco) supplemented with 10% FBS (Invitrogen). Hep3B cells were cultured in MEM (Gibco) containing 10% FBS (Invitrogen). All cells were cultured at 37 °C in 5% CO2.
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