Cortisol elisa kit

The sample was extracted from the serum tube, separated by centrifuge, and 500 µL of the supernatant was sampled into each 1.5 mL tube (Eppendorf AG, Hamburg, Germany). The serum samples were analyzed using a cortisol ELISA kit (MBS701325, MyBioSource) and a haptoglobin ELISA kit (MBS739905, MyBioSource). The concentration of hormones, including cortisol and haptoglobin, in blood was measured using an analytical ELISA machine (PMT49984, BioTek Instruments Synergy Korea Ltd., Winooksi, VT, USA) measuring absorbance at a wavelength of 450 nm. The inter- and intra-assays of coefficients of variance for cortisol were 10% and 10%, while those for haptoglobin were 10% and 8%, respectively.

Blood samples were collected by cardiac puncture and transferred to a collecting tube. Serum was then extracted/separated by centrifugation for 15–20 min at 4°C and 2,000–3,000 rpm. Serum cortisol and ACTH levels were determined by ELISA according to the manufacturers’ instructions using an ACTH ELISA kit (Catalog No. E026Mo, Bioassay Technology Laboratory, Shanghai, China) and a cortisol ELISA kit (Catalog No. MBS269130, MyBioSource, Inc., San Diego, United States) respectively.