Rat anti lamp1 antibody

The following mouse antibodies were used: anti-Rab7 (Sigma), anti-Tsg101 (GeneTex), anti-β-actin (Abcam), anti-GM130 (BD Transduction Laboratories) and anti-prion (SAF32, Cayman chemical; AH6, TSE Resource Center,). The following rabbit antibodies were used: anti-Hrs (Novus Biologicals), anti-TGN38 (AbD Serotec), anti-GFP (Abcam), anti-EEA1 (Cell Signaling), anti-Vps26 (a gift from Juan Bonifacino, Cell Biology Metabolism Program, NICHD, NIH, Bethesda, MD), anti-CI-M6PR (a gift from Linton Traub, Department of Cell Biology, University of Pittsburgh, PA) and anti-Alix (Bethyl Laboratories). Rat anti-LAMP1 antibody (Developmental Studies Hybridoma Bank) was used. PrPc and PrPsc were routinely detected using DyL488, Cy3 and DyL647-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories). Western blots were probed using horseradish peroxidase (HRP)-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories) and InfraRed Dye 680 and 800 secondary antibodies (Li-Cor Bioscience).

RPMI, HBSS, DMEM, EMEM, Trypsin-EDTA and Fetal Bovine Serum (FBS) were purchased from Wisent (Saint-Jean-Baptiste, Canada). Cy3 and Cy5 labeled DNA oligomers were ordered from IDT (Coralville, Iowa). #1.5 thickness round cover slips and 16% paraformaldehyde (PFA) were purchased from Electron Microscopy Supplies (Hatfield, Pennsylvania). HEK 293 and HeLa cells were purchased from ATCC (Manassas, Virginia), SN56 cells were a kind gift from Jane Rylett (University of Western Ontario, Canada). Lipofectamine 2000, Alexa Fluor-labeled secondary Fab antibodies and anti-Golgin97, were purchased form Life Technologies (Burlington, Canada). Dylight-labeled secondary Fab antibodies were purchased from Cedarlane (Burlington, Canada). Cysteamine, mouse anti-HA antibody, rabbit anti-FLAG antibody, Isoproterenol, dibutyrl cyclic AMP, Bovine Serum Albumin (BSA), anti-AP-1γ, and anti-TGN46 were purchased from Sigma-Aldrich (Oakville, Canada). Rat anti-LAMP1 antibody was purchased from the Developmental Studies Hybridoma Bank (Iowa City, Iowa). PolyJet was purchased from Frogga Bio (North York, Canada). All other chemicals were purchased from ThermoFisher (Toronto, Canada). Matlab software was purchased from MathWorks (Natick, Massachusetts). Prism software was purchased from Graphad (La Jolla, California).