400 mhz nmr

Manufactured by JEOL

The 400-MHz NMR is a nuclear magnetic resonance spectrometer that operates at a frequency of 400 MHz. It is used to analyze the molecular structure and properties of chemical compounds by detecting the magnetic properties of atomic nuclei.

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Lab products found in correlation

Dialysis membrane, by Merck Group (1 mentions) Nicolet is50 ftir spectrometer, by Thermo Fisher Scientific (1 mentions)

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400 MHz NMR spectrometer (20 citations) ECS 400 MHz NMR spectrometer (7 citations) ECZR 400 MHz NMR spectrometer (5 citations) ECS-400 400 MHz NMR spectrometer (3 citations) JEOL 400 MHz FT-NMR, (2 citations) ECA 400 MHz NMR spectrometer (2 citations)

3 protocols using 400 mhz nmr

NMR Analysis of Cross-Linked Specimens

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¹H NMR experiments were carried out on 400-MHz NMR (JEOL; Peabody, MA), with 3-(trimethylsilyl)-propionic-2,2,3,3-d4-acid used as the internal standard. Cross-linked specimens were washed with deionized (DI) water and incubated in collagenase solution (1 mL, 20 U/mL) in CaCl₂ (0.36 mM) buffer at pH = 7.4 to digest the polymeric network. Afterward, the solutions were freeze-dried, and like non-cross-linked samples, they were dissolved in D₂O at 40 °C, before the ¹H NMR spectra were obtained.

Sharifi S., Sharifi H., Akbari A., Koza D., Dohlman C.H., Paschalis E.I, & Chodosh J. (2021). Photo-cross-linked Gelatin Glycidyl Methacrylate/N-Vinylpyrrolidone Copolymeric Hydrogel with Tunable Mechanical Properties for Ocular Tissue Engineering Applications. ACS applied bio materials, 4(10), 7682-7691.

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Characterization of GELGYM Hydrogels

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All ¹H-NMR experiments were performed on 400-MHz NMR (JEOL; Peabody, MA) and chemical shifts reported relative to 3-(trimethylsilyl)-propionic-2,2,3,3-d4-acid as the internal standard. A small amount of GELGYM (10 mg) mixed with D₂O (0.5 mL) in an NMR tube was heated for 30 min at 40 °C to fully dissolve the precursor and the spectra were recorded while the sample was maintained at 30 °C. For crosslinked samples, the prepolymer solution (80 μL) was transferred to a cylindrical mold (0.6 mm diameter, and 0.2 mm depth), crosslinked, and washed with water. Afterward, the samples were incubated in collagenase solution (2 mL, 10 U/ml), containing Tris-HCl buffer (0.1 M) with a pH of 7.4 supplemented with CaCl₂ (5 mM) at 37 °C to digest the hydrogel and form a homogenous solution. Then, the solutions were freeze-dried to eliminate the water and similar to non-crosslinked samples were dissolved in D₂O at 30 °C and their ¹H-NMR spectra were acquired.

Sharifi S., Islam M.M., Sharifi H., Islam R., Koza D., Reyes-Ortega F., Alba-Molina D., Nilsson P.H., Dohlman C.H., Mollnes T.E., Chodosh J, & Gonzalez-Andrades M. (2021). Tuning gelatin-based hydrogel towards bioadhesive ocular tissue engineering applications. Bioactive Materials, 6(11), 3947-3961.

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Synthesis and Characterization of G-GMA Macromer

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To synthesize G-GMA, 10.0 g of gelatin from porcine skin (~300 g of Bloom, type A) was dissolved in 100 mL of PBS, followed by the addition of 10 mL of glycidyl methacrylate. The reaction mixture was stirred for 5 h at 45 °C, then diluted with 100 mL distilled water, and dialyzed for 7 days by using a dialysis membrane (molecular weight cutoff of 14 kDa; Sigma-Aldrich) to eliminate the unreacted reagents and smaller molecular weight by products. Afterward, the solution was freeze-dried for 4 days to afford a foam-like G-GMA macromer. To chemically characterize the macromer, we performed ¹H NMR (400 MHz NMR; JEOL; Peabody, MA, with 3-(trimethylsilyl)propionic2,2,3,3-d₄ acid used as the internal standard) and FT-IR (Nicolet iS50 FTIR spectrometer; Thermo Scientific, Cambridge, MA) (Figures S1 and S2)

Sharifi S., Sharifi H., Akbari A., Dohlman C.H., Paschalis E.I., Gonzalez-Andrades M., Kong J, & Chodosh J. (2021). Graphene-Lined Porous Gelatin Glycidyl Methacrylate Hydrogels: Implications for Tissue Engineering. ACS applied nano materials, 4(11), 12650-12662.

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