Immunohistochemistry was performed on 2 μm paraffin sections of normal human skin (n = 8) and of lesional psoriatic skin (n = 8), using an anti-human CXCR4 antibody (R&D Systems) or control mouse IgG (Sigma) followed by a biotinylated anti-mouse secondary antibody (Vector). The Vectastain ABC and the AEC (3-amino-9-ethylcarbazole) substrate kits (Vector) were used for chromogenic detection.
Anti human cxcr4 antibody
Manufactured by R&D Systems
The Anti-human CXCR4 antibody is a laboratory reagent used for the detection and study of the CXCR4 protein. CXCR4 is a chemokine receptor that plays a role in various cellular processes. This antibody can be used in techniques such as flow cytometry, immunohistochemistry, and Western blotting to identify and analyze CXCR4 expression in samples.
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Lab products found in correlation
Biotinylated anti mouse secondary antibody, by Vector Laboratories (1 mentions)
Vectastain abc, by Vector Laboratories (1 mentions)
Control mouse igg, by Merck Group (1 mentions)
Protease inhibitor cocktail, by Merck Group (1 mentions)
Ecl chemiluminescence detection kit, by GE Healthcare (1 mentions)
Laemmli sample buffer, by Bio-Rad (1 mentions)
β actin antibody, by Merck Group (1 mentions)
2 protocols using anti human cxcr4 antibody
1
Immunodetection of CXCR4 in Psoriatic Skin
2
Western Blot Analysis of CXCR4 Expression
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Tissues were homogenized in cold RIPA buffer with protease inhibitor cocktails (Sigma-Aldrich, St. Louis, MO). Tissue debris was removed by centrifugation. Equal amounts of 1× Laemmli sample buffer (Bio-Rad Laboratories, Hercules, CA) were added to the samples, and the mixtures were heated to 95°C. The samples were then subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto nitrocellulose membranes. Finally, each membrane was incubated with anti-human CXCR4 antibody according to the manufacture instructions (R&D Systems, Minneapolis, MN) for 1 h and developed by using the ECL chemiluminescence detection kit (GE Healthcare Life Sciences, Piscataway, NJ). Membranes were incubated with β-actin antibody (Sigma-Aldrich, St. Louis, MO) as a loading control.
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