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Hplc grade tert butyl methyl ether mtbe

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HPLC grade tert-Butyl methyl ether (MTBE) is a colorless, volatile organic compound used as a solvent in high-performance liquid chromatography (HPLC) applications. It serves as a mobile phase component in HPLC systems, facilitating the separation and analysis of chemical compounds.

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Lab products found in correlation

Ammonium formate, by Thermo Fisher Scientific (4 mentions) Methanol, by Thermo Fisher Scientific (4 mentions) Formic acid, by Thermo Fisher Scientific (3 mentions) Lc ms grade acetonitrile, by Thermo Fisher Scientific (3 mentions) Cerulenin, by Merck Group (1 mentions) Fluvastatin sodium hydrate, by Merck Group (1 mentions) Uridine, by Merck Group (1 mentions) Doxycycline, by Merck Group (1 mentions) Trypsin, by Thermo Fisher Scientific (1 mentions) Nd 646, by MedChemExpress (1 mentions) Isopropanol, by Thermo Fisher Scientific (1 mentions) Ethylene diamine tetraacetic acid (edta), by Thermo Fisher Scientific (1 mentions) Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions) U 14c glucose, by PerkinElmer (1 mentions) U 14c glutamine, by PerkinElmer (1 mentions) Apo transferrin, by Merck Group (1 mentions) Geneticin, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin mixture, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

HPLC grade methyl tert-butyl ether Tert-butyl methyl ether (MTBE), Methyl tert-butyl ether Tert-butyl methyl ether HPLC grade

4 protocols using hplc grade tert butyl methyl ether mtbe

1

Lipid Analysis by LC-MS

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LC-MS grade acetonitrile, methanol, water, isopropanol, formic acid, and ammonium formate were purchased from Fisher Scientific (Pittsburg, PA). HPLC grade tert-Butyl methyl ether (MTBE) was purchased from Sigma Aldrich (St. Louis, MO). Lipid standards were purchased from Avanti Polar Lipids, Inc. (Alabaster, AL). The lipid standards included Splash Lipidomix and a number of individual lipid standards.
Jones J.W., Sarkar C., Lipinski M.M, & Kane M.A. (2019). Detection and Structural Characterization of Ether Glycerophosphoethanolamine from Cortical Lysosomes Following Traumatic Brain Injury Using UPLC-HDMSE. Proteomics, 19(18), e1800297.
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2

Profiling Metabolic Pathways in Cell Lines

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Na2S, nonahydrate (99.99% purity), glucose, hydrocortisone, insulin, apo-transferrin, sodium selenite, sodium orthovanadate, uridine (cell culture grade), Protease Inhibitor Cocktail for use with mammalian cell and tissue extracts, puromycin, cerulenin, fluvastatin sodium hydrate, doxycycline, RIPA buffer, HPLC grade tert-Butyl methyl ether (MTBE), and chloroform were from Sigma. TOFA and ND-646 were from MedChemExpress. Dulbecco's modified Eagle's medium (DMEM) (with 4.5 g/l glucose, glutamine, and 110 mg/l sodium pyruvate), RPMI 1640 with glutamine, medium 199, fetal bovine serum (FBS), penicillin/streptomycin mixture, trypsin, EDTA, PBS, and Dulbecco's phosphate-buffered saline medium (DPBS) were from Gibco. Geneticin was purchased from Life Technologies. Hepes and LC-MS grade acetonitrile, methanol, water, isopropanol, and ammonium formate were from Fisher, and Eagle's minimal essential medium (EMEM) was from Lonza. [U-14C]-glucose (263.0 mCi/mmol) and [U-14C]-glutamine (281.0 mCi/mmol) were from PerkinElmer. EquiSPLASH lipid standard (#330731) was purchased from Avanti Polar Lipids, Inc.
Carballal S., Vitvitsky V., Kumar R., Hanna D.A., Libiad M., Gupta A., Jones J.W, & Banerjee R. (2021). Hydrogen sulfide stimulates lipid biogenesis from glutamine that is dependent on the mitochondrial NAD(P)H pool. The Journal of Biological Chemistry, 297(2), 100950.
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3

Lipid Standards for LC-MS Analysis

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LC-MS grade acetonitrile, methanol, water, isopropanol, formic acid and ammonium formate were purchased from Fisher Scientific (Pittsburg, PA). HPLC grade tert-Butyl methyl ether (MTBE) was purchased from Sigma Aldrich (St. Louis, MO). Lipid standards were purchased from Avanti Polar Lipids, Inc. (Alabaster, AL). The lipid standards included ceramide/sphingoid internal standard mixture I (Avanti Polar Lipids, LM6002).
Li L., Wang H, & Jones J.W. (2020). Sphingolipid metabolism as a marker of hepatotoxicity in drug-induced liver injury. Prostaglandins & other lipid mediators, 151, 106484.
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4

Lipidomics Mass Spectrometry Protocol

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LC–MS-grade acetonitrile, methanol, water, n-propanol, formic acid, and ammonium formate were purchased from Fisher Scientific (Pittsburgh, PA). HPLC-grade tert-butyl methyl ether (MTBE) and chloroform (CHCl3) were purchased from Sigma-Aldrich (St. Louis, MO). 99% purity 2,5-dihydroxybenzoic acid (DHB) was purchased from Acros Organic (Fair Lawn, NJ). 2′,4′,6′-Trihydroxyacetophenone (THAP, MALDI MS grade) and lithium chloride (LiCl) were purchased from Sigma-Aldrich (St. Louis, MO). Lipid standards were purchased from Avanti Polar Lipids, Inc. (Alabaster, AL). The lipid standards included EquiSPLASH LIPIDOMIX Quantitative Mass Spec Internal Standard (330731). Additional lipid standards included glucosyl ceramide (d18:1/16:0) (GlcCer(d18:1/16:0)) (860539) and galactosyl ceramide (d18:1/16:0) (GalCer(d18:1/16:0)) (860521).
Tran A., Monreal I.A., Moskovets E., Aguilar H.C, & Jones J.W. (2021). Rapid Detection of Viral Envelope Lipids Using Lithium Adducts and AP-MALDI High-Resolution Mass Spectrometry. Journal of the American Society for Mass Spectrometry, 32(9), 2322-2333.
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