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Antibiotic antimicotic

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Antibiotic-antimicotic is a sterile solution containing a combination of antibiotics and antimycotics, commonly used in cell culture applications to prevent microbial contamination.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions) Pen strep, by Thermo Fisher Scientific (3 mentions) Hepes, by Thermo Fisher Scientific (2 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions) Glutamax, by Thermo Fisher Scientific (2 mentions) Mab2871, by R&D Systems (2 mentions) Clonepix fl, by Thermo Fisher Scientific (2 mentions) Neurocult ns a proliferation kit, by STEMCELL (1 mentions) Human recombinant epidermal growth factor, by STEMCELL (1 mentions) Plasmocin, by InvivoGen (1 mentions) Heparin, by STEMCELL (1 mentions) B cell isolation kit 2, by Miltenyi Biotec (1 mentions) Sepmate tube, by STEMCELL (1 mentions) Glutamine, by Thermo Fisher Scientific (1 mentions) Dmem medium, by Thermo Fisher Scientific (1 mentions) Insulin transferrin selenium, by Thermo Fisher Scientific (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Collagenase, by Thermo Fisher Scientific (1 mentions) Collagenase, by Fujifilm (1 mentions) Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions)

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Antibiotics and antimicotics (2 citations) Antibiotic-antimicotic solution (1 citations)

15 protocols using antibiotic antimicotic

1

Biocompatible Gelatin-Hyaluronic Acid Hydrogel

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Gelatin from porcine skin (type A, bloom 300), methacrylic anhydride (MAA) (94%), HA sodium salt (Mw 1.5-1.8 × 10 6 ), sodium (meta)periodate (99%) and lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP) were purchased from Sigma Aldrich (St. Louis, MO, USA). Dulbecco's modified Eagle's medium (DMEM) and antibiotic-antimicotic were obtained from Gibco, Life Technologies (USA).
MC3T3-E1 cells obtained from mouse calvaria was purchased from Sigma Aldrich (Belgium), and were cultured in DMEM with 10% fetal bovine serum (Gibco, USA) and 1% antibiotic-antimicotic at 37 • C with a humidified 5% CO 2 atmosphere. Live/dead TM cell viability assay kit was purchased from Invitrogen, ThermoFisher Scientific (USA). MTS CellTiter ® 96 aqueous one solution cell proliferation assay kit was purchased from Promega (Belgium).
Anand R., Salar Amoli M., Huysecom A.S., Amorim P.A., Agten H., Geris L, & Bloemen V. (2022). A tunable gelatin-hyaluronan dialdehyde/methacryloyl gelatin interpenetrating polymer network hydrogel for additive tissue manufacturing. Biomedical materials (Bristol, England), 17(4).
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2

Articular Cartilage Biopsy and oACh Isolation

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Two oACh donors (oACh 1 and oACh 2) were used in the cartilage defect repair component of this study. Articular cartilage biopsies were harvested from the femoral trochlear grooves. The knee joint capsule was accessed via a medial parapatellar incision (~ 5 cm). The patella was displaced laterally and retained in a proximal position with small wound retractors. The joint capsule was opened to expose the femoral trochlear groove. Five thin (~ 1 mm thick and 4–6 mm wide) shavings of articular cartilage were aseptically removed with a scalpel and transferred to a sterile specimen jar containing LG-DMEM with PenStrep and 1X Antibiotic-Antimycotic (Anti-Anti; Thermo Fisher Scientific). The biopsied tissues were placed in a Petri dish and chopped up coarsely. The tissue was resuspended in 10 mL of digestion solution containing type II clostridial collagenase (~ 430 U/mL; Sigma-Aldrich), PenStrep, and Antibiotic-Antimicotic (Gibco) and incubated overnight in a normoxic incubator (20% O2) with 5% CO2 at 37 °C. Following digestion, the cells were washed twice by centrifugation (500×g) with LG-DMEM containing 10% FBS. oACh were grown in a hypoxic (2% O2) incubator in growth medium as described above for oBMSC.
Futrega K., Music E., Robey P.G., Gronthos S., Crawford R., Saifzadeh S., Klein T.J, & Doran M.R. (2021). Characterisation of ovine bone marrow-derived stromal cells (oBMSC) and evaluation of chondrogenically induced micro-pellets for cartilage tissue repair in vivo. Stem Cell Research & Therapy, 12, 26.
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3

Culturing Human Glioblastoma Cell Lines

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The human glioblastoma cell line U251 was kindly provided by the laboratory of Dr. Blasberg (MSK, New York, NY). Cells were grown in Eagle’s Minimal Essential Medium (MEM), 10% (vol/vol) heat inactivated fetal bovine serum, 100 IU2 penicillin, and 100 μg/ml streptomycin, purchased from the culture media preparation facility at MSK (New York, NY). TS543 cells are a patient-derived glioblastoma stem line kindly provided by the laboratory of Dr. Mellinghoff (MSK, New York, NY). These cells were grown in suspension in NeuroCultTM NS-A Proliferation Kit with proliferation supplement (StemCell Technologies, Cat 05751), 20 ng/mL Recombinant Human Epidermal Growth Factor (EGF) (StemCell Technologies, Cat 02633), 10 ng/mL Recombinant Human Basic Fibroblast Growth Factor (Bfgf) (StemCell Technologies, Cat 02634), 2 μg/mL Heparin (StemCell Technologies, Cat 07980), 1× antibiotic-antimicotic (Life Technologies Gibco, Cat 15240–062), 2.5 mg/mL Plasmocin (InvivoGen, Cat ant-mpp). All cells were tested for mycoplasma contamination.
Pirovano G., Jannetti S.A., Carter L.M., Sadique A., Kossatz S., Guru N., De Souza Franca P.D., Maeda M., Zeglis B.M., Lewis J.S., Humm J.L, & Reiner T. (2020). Targeted brain tumor radiotherapy using an Auger emitter. Clinical cancer research : an official journal of the American Association for Cancer Research, 26(12), 2871-2881.
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4

Establishing Lymphoblastoid Cell Lines

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Lymphoblastoid cell lines (LCLs) derived from control study participants without lupus were generated as previously described (Rasmussen et al., 2011 (link)) and maintained at 37°C in RPMI 1640 media containing 10% heat-inactivated fetal bovine serum (FBS) and antibiotic/antimicotic (Gibco/Life Technologies, Grand Island, NY). Peripheral blood mononucelar cells (PBMCs) were isolated using Ficoll-gradient centrifugation in SepMate tubes (Stemcell Technologies, Vancouver, BC, Canada) from fresh blood collected in acid citrate dextrose (ACD) tubes with informed consent from healthy donors. B cells were isolated using the B-cell isolation kit-II (Miltenyi Biotech, Auburn, CA).
Vaughn S.E., Foley C., Lu X., Patel Z.H., Zoller E.E., Magnusen A.F., Williams A.H., Ziegler J.T., Comeau M.E., Marion M.C., Glenn S.B., Adler A., Shen N., Nath S., Stevens A.M., Freedman B.I., Tsao B.P., Jacob C.O., Kamen D.L., Brown E.E., Gilkeson G.S., Alarcón G.S., Reveille J.D., Anaya J.M., James J.A., Moser K.L., Criswell L.A., Vilá L.M., Alarcón-Riquelme M.E., Petri M., Scofield R.H., Kimberly R.P., Ramsey-Goldman R., Binjoo Y., Choi J., Bae S.C., Boackle S.A., Vyse T.J., Guthridge J.M., Namjou B., Gaffney P.M., Langefeld C.D., Kaufman K.M., Kelly J.A., Harley I.T., Harley J.B, & Kottyan L.C. (2015). Lupus risk variants in the PXK locus alter B-cell receptor internalization. Frontiers in Genetics, 5, 450.
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5

ARPE-19 Starvation and Mouse Fasting Protocol

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Adult Retinal Pigment Epithelial cell line-19 (ARPE-19) cells were cultured in DMEM/F12 with L-Glutamine and 15 mM HEPES (Gibco, Thermo Fisher Scientific) along with 10% Fetal Bovine Serum (Hyclone, GE Healthcare Life Sciences) and 1% Antibiotic-Antimicotic (Gibco, Thermo Fisher Scientific). For starvation, the cells were cultured in Earle’s Balanced Salt Solution with calcium and magnesium for 24–72 h. C57BL/6 J mice were withheld food for 24–72 h and the mice were provided with water during this period. The experimental procedures were approved by the Institutional Animal Care and Use Committee, Indiana University/School of Optometry and conformed to the ARVO Statement for the Use of Animals in Ophthalmologic and Vision Research.
Pan H.Y., Alamri A.H, & Valapala M. (2019). Nutrient deprivation and lysosomal stress induce activation of TFEB in retinal pigment epithelial cells. Cellular & Molecular Biology Letters, 24, 33.
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6

Culturing Human Glioblastoma LN-229 Cells

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Human glioblastoma cell line LN-229 (ATCC: CRL-2611) was cultured in DMEM medium (Gibco, Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (PAA, Germany), glutamine and antibiotic-antimicotic (Gibco).
Duhart J.M., Brocardo L., Caldart C.S., Marpegan L, & Golombek D.A. (2017). Circadian Alterations in a Murine Model of Hypothalamic Glioma. Frontiers in Physiology, 8, 864.
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7

Porcine Oocyte Maturation Protocol

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Ovaries were collected from gilts at a local slaughterhouse called "La Pompeya" located in the province of Buenos Aires and transported to the laboratory at around 25–30°C within 3h of collection. Cumulus-oocyte complexes (COCs) from follicles 3 to 6mm in diameter were aspirated using an 18-gauge needle attached to a 10mL disposable syringe. Compact COCs were selected and matured in 100μL drops of tissue culture medium (TCM-199- 31100–035; Gibco, Grand Island, NY, USA) under mineral oil (M8410), supplemented with 0.3mM sodium pyruvate (P2256), 100μM cysteamine (M9768), 5μg/ml myoinositol, 1μg/ml insulin transferrin selenium (ITS; product no. 51300–044; Gibco), 1% antibiotic-antimicotic (15240–096 Gibco), 10% (v/v) porcine follicular fluid, 5 ng/ml basic fibroblast growth factor (bFGF) and 10μg/ml of follicle-stimulating hormone (FSH) (NIH-FSH-P1Folltropin, Bioniche, Caufield Junction Caufield North, Victoria, Australia). Maturation was performed at 39°C in a humidified atmosphere of 6.5% CO2 in 90% air for 42-44h.
Buemo C.P., Gambini A., Moro L.N., Hiriart M.I., Fernández-Martín R., Collas P, & Salamone D.F. (2016). Embryo Aggregation in Pig Improves Cloning Efficiency and Embryo Quality. PLoS ONE, 11(2), e0146390.
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8

HL-60 Cell Culture and ATRA Treatment

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Human myeloblastic leukemia cells (HL-60 cells) were grown in a humidified atmosphere of 5% CO2 at 37 °C and maintained in RPMI 1640 from Gibco (Carlsbad, CA) supplemented with 5% heat inactivated fetal bovine serum from Hyclone (Logan, UT) and 1× antibiotic/antimicotic (Gibco, Carlsbad, CA). Cells were cultured in constant exponential growth65 (link). Experimental cultures were initiated at 0.1×106 cells/mL 24 hr prior to ATRA treatment; if indicated, cells were also treated with GW5074 (2 μM) 18 hr before ATRA treatment. For the cell culture washout experiments, cells were treated with ATRA for 24 hr, washed 3x with prewarmed serum supplemented culture medium to remove ATRA, and reseeded in ATRA-free media as described. Western blot analysis was performed at incremental time points after removal of ATRA.
Tasseff R., Jensen H.A., Congleton J., Dai D., Rogers K.V., Sagar A., Bunaciu R.P., Yen A, & Varner J.D. (2017). An Effective Model of the Retinoic Acid Induced HL-60 Differentiation Program. Scientific Reports, 7, 14327.
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9

Stromal Vascular Fraction Extraction from Rat Adipose

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SVF preparation came from fat tissue (adipose) collected from all rat donors. Fat tissues were collected from the left and right inguinal areas. The fats were then washed with phosphate buffer salts (PBS; Gibco-BRL, Grand Island, NY, USA), chopped small until smooth, and then inserted into a tube. We then added 0.15% collagenase (Wako Pure Chemical Industries, Ltd., Osaka, Japan) into the fat contained-tube and incubated the fat at 37 °C for 30 min. Next, we inserted media control Dulbecco Modified Eagle Media (DMEM; GIBCO-BRL) with 10% Fetal Bovine Serum (FBS; GIBCO-BRL) and 1% antibiotic-antimicotic (GIBCO-BRL) to neutralize collagenase activation and then centrifuged at 1500 rpm for 5 min. The cell pellet was resuspended with aquadest. SVFs were then calculated using Trypan Blue and a Nebauer counting chamber. A total of 50,000 SVFs combined with 0.5 cc aquadest were then transferred to an eppendorf tube for the final product [14 (link)].
Sirowanto I., Josh F., S.u.l.m.i.a.t.i., A.h.m.a.d.w.i.r.a.w.a.n., Zainuddin A.A, & Faruk M. (2021). The effect of Platelet-Rich Plasma and Stromal Vascular Fraction combination on Epidermal Growth Factor serum level for anal trauma healing in the Wistar rat model. Annals of Medicine and Surgery, 70, 102773.
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10

Evaluation of Fatty Acid-Loaded Gauzes

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To evaluate the biological performance of gauzes loaded with fatty acids, cell culture studies were performed with L929, a mouse fibroblast of connective tissue cell line (European Collection of Cell Cultures (ECCC), UK). Cells were cultured in Dulbecco's modified Eagle's medium (DMEM, Sigma, USA), supplemented by 10 % heat-inactivated fetal bovine serum (FBS, Biochrome AG, Germany) and 1 % antibioticantimicotic (Gibco, USA). The cytotoxicity was studied by analysing the effect of the extracts of gauzes on the cell's metabolism, which is in accordance with ISO/EN 10993 guidelines. In addition, the gauzes with and without fatty acids were also placed in contact with cell monolayers. The morphological changes of cells after 24 hours in contact with extracts and gauzes was observed by optic microscopy (AxiovertA1, Zeiss, Germany).
The cell viability was determined by CellTiter 96® Aqueous One Solution Cell Proliferation Assay. The amount of formazan product was measured by absorbance at a wavelength of 490 nm using a microplate spectrophotometer (Bio-TEK, USA).
Silva J.M., Akkache S., Araújo A.C., Masmoudi Y., Reis R.L., Badens E, & Duarte A.R.C. (2019). Development of innovative medical devices by dispersing fatty acid eutectic blend on gauzes using supercritical particle generation processes. Materials science & engineering. C, Materials for biological applications, 99.
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